搜索结果: 'EasySep Release'

EasySep™非人灵长类T细胞分选试剂盒 通过免疫磁珠负选从包括恒河猴在内的非人灵长类中分离出无磁珠标记的T细胞
EasySep™非人灵长类CD4+ T细胞分选试剂盒
对来源于恒河猴或食蟹猴 PBMCs 的非人灵长类 CD4⁺ T 细胞进行免疫磁珠负选分离
EasySep™非人灵长类CD8+ T细胞分选试剂盒
对来源于恒河猴或食蟹猴 PBMCs 的非人灵长类 CD8⁺ T 细胞进行免疫磁珠负选分离
Gomez AM et al. (MAR 2015) The Journal of Immunology 194 5 2300--8

HIV-1-triggered release of type I IFN by plasmacytoid dendritic cells induces BAFF production in monocytes.

HIV-1 infection leads to numerous B cell abnormalities,including hypergammaglobulinemia,nonspecific B cell activation,nonspecific class switching,increased cell turnover,breakage of tolerance,increased immature/transitional B cells,B cell malignancies,as well as a loss of capacity to generate and maintain memory,all of which contribute to a global impairment of the immune humoral compartment. Several cytokines and soluble factors,which are increased in sera of HIV-1-infected individuals,have been suggested to directly or indirectly contribute to these B cell dysfunctions,and one of these is the B cell-activating factor (BAFF). We report in this study that HIV-1 (X4- and R5-tropic) upregulates BAFF expression and secretion by human monocytes. Moreover,we show that the virus-mediated production of BAFF by monocytes relies on a type I IFN response by a small percentage of plasmacytoid dendritic cells (pDCs) present in the monocyte cultures. HIV-1-induced type I IFN by pDCs triggers BAFF production in both classical and intermediate monocytes,but not in nonclassical monocytes,which nonetheless display a very strong basal BAFF production. We report also that basal BAFF secretion was higher in monocytes obtained from females compared with those from male donors. This study provides a novel mechanistic explanation for the increased BAFF levels observed during HIV-1 infection and highlights the importance of pDC/monocyte crosstalk to drive BAFF secretion. View Publication

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19062

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EasySep™人浆细胞样DC富集试剂盒

RoboSep™ 人浆细胞样DC富集试剂盒含滤芯吸头

EasySep™人单核细胞富集试剂盒（不去除CD16）

RoboSep™ 人单核细胞富集试剂盒（不去除CD16）含滤芯吸头

EasySep™人单核细胞富集试剂盒（不去除CD16）
4:30

视频
How to Isolate Cells Directly from Whole Blood Using the EasySep™ Purple/Silver Magnets

发布日期: 03/09/2015
1:44

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Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct

发布日期: 10/10/2014
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How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, EasyEights Magnet

发布日期: 03/09/2015
5:17

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How to Isolate Cells Directly from Whole Blood Using the Easy 50 EasySep™ Magnet: 30-Minute Protocol

发布日期: 03/09/2015
产品手册
EasySep™ Direct 仅需20分钟，从全血中分选细胞

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品牌：

EasySep

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EasySep™ Direct 人Naïve B细胞分选试剂盒

EasySep™ Direct人总淋巴细胞分选试剂盒

EasySep™ Direct人T细胞分选试剂盒

EasySep™ Direct人CD4+ T细胞分选试剂盒

RoboSep™ Direct人CD4+ T细胞分选试剂盒

EasySep™ Direct人CD8+ T细胞分选试剂盒

EasySep™ Direct 人B-CLL细胞分选试剂盒

EasySep™ Direct人中性粒细胞分选试剂盒

EasySep™ Direct人单核细胞分选试剂盒

EasySep™ Direct人B细胞分选试剂盒

RoboSep™ Direct人B细胞分选试剂盒

EasySep™ Direct人NK细胞分选试剂盒

EasySep™ Direct 人CTC富集试剂盒

EasySep™ Direct人Pan-粒细胞分选试剂盒

RoboSep™ Direct人总淋巴细胞分选试剂盒
挂图
The Immune Response to HIV Poster Summary of how HIV subverts the immune response to establish a chronic infection
科学海报
Immunomagnetic Purification of Human Central and Effector Memory T Cell Subsets in 45 Minutes

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Conference：

KEYSTONE 2018

产品号#：

17865

17869

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EasySep™ 人中央和效应记忆CD4+ T细胞分选试剂盒

EasySep™ 人中央和效应记忆CD8+ T细胞分选试剂盒
Apps R et al. (MAY 2016) Cell Host & Microbe 19 5 686--95

HIV-1 Vpu Mediates HLA-C Downregulation.

Many pathogens evade cytotoxic T lymphocytes (CTLs) by downregulating HLA molecules on infected cells,but the loss of HLA can trigger NK cell-mediated lysis. HIV-1 is thought to subvert CTLs while preserving NK cell inhibition by Nef-mediated downregulation of HLA-A and -B but not HLA-C molecules. We find that HLA-C is downregulated by most primary HIV-1 clones,including transmitted founder viruses,in contrast to the laboratory-adapted NL4-3 virus. HLA-C reduction is mediated by viral Vpu and reduces the ability of HLA-C restricted CTLs to suppress viral replication in CD4+ cells in vitro. HLA-A/B are unaffected by Vpu,and primary HIV-1 clones vary in their ability to downregulate HLA-C,possibly in response to whether CTLs or NK cells dominate immune pressure through HLA-C. HIV-2 also suppresses HLA-C expression through distinct mechanisms,underscoring the immune pressure HLA-C exerts on HIV. This viral immune evasion casts new light on the roles of CTLs and NK cells in immune responses against HIV. View Publication

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产品号#：

17952

17952RF

100-0696

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EasySep™人CD4+ T细胞分选试剂盒

RoboSep™ 人CD4+ T细胞分选试剂盒

EasySep™人CD4+ T细胞分离试剂盒