搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

科学海报
A Robust, Xeno- and Feeder-Free Culture System for Expansion of Human Peripheral Blood NK Cells

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Conference：

AAI 2022

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科学海报
Positive Selection of Hematopoietic Stem and Progenitor Cells from Adult Mouse Bone Marrow

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Conference：

ASH 2003

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科学海报
Generation of T Cells from Hematopoietic Stem and Progenitor Cells in a Stroma-Free, Serum-Free Culture System

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产品号#：

09940

09605

09915

09925

09930

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StemSpan™ T细胞生成试剂盒

StemSpan™ SFEM II

StemSpan™淋系祖细胞扩增添加物（10X）

StemSpan™淋系祖细胞分化包板材料（100X）

StemSpan™ T细胞祖细胞成熟添加物（10X）
Hultquist JF et al. (OCT 2016) Cell reports 17 5 1438--1452

A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.

New genetic tools are needed to understand the functional interactions between HIV and human host factors in primary cells. We recently developed a method to edit the genome of primary CD4(+) T cells by electroporation of CRISPR/Cas9 ribonucleoproteins (RNPs). Here,we adapted this methodology to a high-throughput platform for the efficient,arrayed editing of candidate host factors. CXCR4 or CCR5 knockout cells generated with this method are resistant to HIV infection in a tropism-dependent manner,whereas knockout of LEDGF or TNPO3 results in a tropism-independent reduction in infection. CRISPR/Cas9 RNPs can furthermore edit multiple genes simultaneously,enabling studies of interactions among multiple host and viral factors. Finally,in an arrayed screen of 45 genes associated with HIV integrase,we identified several candidate dependency/restriction factors,demonstrating the power of this approach as a discovery platform. This technology should accelerate target validation for pharmaceutical and cell-based therapies to cure HIV infection. View Publication

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产品号#：

19052

19052RF

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EasySep™人CD4+ T细胞富集试剂盒

RoboSep™ 人CD4+ T细胞富集试剂盒含滤芯吸头
科学海报
Fully Automated Magnetic Labeling and Separation of Hematopoietic Cells from Multiple Samples

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Conference：

BSI 2005

产品号#：

18000

产品名：

EasySep™磁极
科学海报
Workflow Solutions for Human T Cell Isolation and Expansion

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Conference：

CCIC 2015

产品号#：

10970

10990

10971

10991

10981

17951

17951RF

产品名：

ImmunoCult™ 人CD3/CD28/CD2 T细胞激活剂

ImmunoCult™ 人CD3/CD28/CD2 T细胞激活剂

ImmunoCult™ 人CD3/CD28 T细胞激活剂

ImmunoCult™ 人CD3/CD28 T细胞激活剂

ImmunoCult™ XF 人T细胞扩增培养基，500 mL

EasySep™人T细胞分选试剂盒

RoboSep™ 人T细胞分选试剂盒
产品手册
Isolate Human Immune Cells

产品类型：

品牌：

EasySep

产品号#：

10970

10990

10971

10991

10981

17851

17851RF

17852

17852RF

17853

17853RF

17854

17854RF

17855

17855RF

17951

17951RF

17952

17952RF

17953

17953RF

17955

17955RF

17954

17954RF

17254

17254RF

10973

10975

10977

18170

18170RF

17883

17883RF

产品名：

ImmunoCult™ 人CD3/CD28/CD2 T细胞激活剂

ImmunoCult™ 人CD3/CD28/CD2 T细胞激活剂

ImmunoCult™ 人CD3/CD28 T细胞激活剂

ImmunoCult™ 人CD3/CD28 T细胞激活剂

ImmunoCult™ XF 人T细胞扩增培养基，500 mL

EasySep™人CD3正选试剂盒II

RoboSep™ 人CD3正选试剂盒II

EasySep™人CD4正选试剂盒II

RoboSep™ 人CD4正选试剂盒II

EasySep™人CD8正选试剂盒 II

RoboSep™ 人CD8正选试剂盒 II

EasySep™人CD19正选试剂盒II

RoboSep™ 人CD19正选试剂盒II

EasySep™人CD56正选试剂盒 II

RoboSep™ 人CD56正选试剂盒 II

EasySep™人T细胞分选试剂盒

RoboSep™ 人T细胞分选试剂盒

EasySep™人CD4+ T细胞分选试剂盒

RoboSep™ 人CD4+ T细胞分选试剂盒

EasySep™人CD8+ T细胞分选试剂盒

RoboSep™ 人CD8+ T细胞分选试剂盒

EasySep™人NK细胞分选试剂盒

RoboSep™ 人NK细胞分选试剂盒

EasySep™人B细胞分选试剂盒

RoboSep™ 人B细胞分选试剂盒

EasySep™ 人Naïve B细胞分选试剂盒

RoboSep™ 人Naïve B细胞分选试剂盒

ImmunoCult™ 人Th1分化添加剂

ImmunoCult™ 人Th2分化添加剂

ImmunoCult™ 人Treg分化添加剂

EasySep™红细胞去除试剂 - 10mL

RoboSep™ 红细胞去除试剂

EasySep™人CD2正选试剂盒II

RoboSep™ 人CD2正选试剂盒II
52:23

线上讲座
Human iPSC BBB Models for Studying Immune Cell Interactions

发布日期: 11/01/2022
Webb CF et al. (MAR 2011) Molecular and cellular biology 31 5 1041--53

The ARID family transcription factor bright is required for both hematopoietic stem cell and B lineage development.

Bright/Arid3a has been characterized both as an activator of immunoglobulin heavy-chain transcription and as a proto-oncogene. Although Bright expression is highly B lineage stage restricted in adult mice,its expression in the earliest identifiable hematopoietic stem cell (HSC) population suggests that Bright might have additional functions. We showed that textgreater99% of Bright(-/-) embryos die at midgestation from failed hematopoiesis. Bright(-/-) embryonic day 12.5 (E12.5) fetal livers showed an increase in the expression of immature markers. Colony-forming assays indicated that the hematopoietic potential of Bright(-/-) mice is markedly reduced. Rare survivors of lethality,which were not compensated by the closely related paralogue Bright-derived protein (Bdp)/Arid3b,suffered HSC deficits in their bone marrow as well as B lineage-intrinsic developmental and functional deficiencies in their peripheries. These include a reduction in a natural antibody,B-1 responses to phosphocholine,and selective T-dependent impairment of IgG1 class switching. Our results place Bright/Arid3a on a select list of transcriptional regulators required to program both HSC and lineage-specific differentiation. View Publication

产品类型：

产品号#：

03434

03444

产品名：

MethoCult™GF M3434

MethoCult™GF M3434
Guia S et al. (MAY 2008) Blood 111 10 5008--16

A role for interleukin-12/23 in the maturation of human natural killer and CD56+ T cells in vivo.

Natural killer (NK) cells have been originally defined by their naturally occurring" effector function. However� View Publication

产品类型：

产品号#：

15025

15065

产品名：

RosetteSep™人NK细胞富集抗体混合物

RosetteSep™人NK细胞富集抗体混合物
技术公告
Expansion and Differentiation of Hematopoietic Stem and Progenitor Cells into Natural Killer (NK) Cells Using StemSpan™ Medium and Supplements

产品类型：

细胞类型：

NK 细胞，造血干/祖细胞

产品号#：

09605

09915

09925

09950

09960

产品名：

StemSpan™ SFEM II

StemSpan™淋系祖细胞扩增添加物（10X）

StemSpan™淋系祖细胞分化包板材料（100X）

StemSpan™ NK细胞分化添加物（100X）

StemSpan™ NK细胞生成试剂盒
Ng Y-S et al. (OCT 2004) The Journal of experimental medicine 200 7 927--34

Bruton's tyrosine kinase is essential for human B cell tolerance.

Most polyreactive and antinuclear antibodies are removed from the human antibody repertoire during B cell development. To elucidate how B cell receptor (BCR) signaling may regulate human B cell tolerance,we tested the specificity of recombinant antibodies from single peripheral B cells isolated from patients suffering from X-linked agammaglobulinemia (XLA). These patients carry mutations in the Bruton's tyrosine kinase (BTK) gene that encode an essential BCR signaling component. We find that in the absence of Btk,peripheral B cells show a distinct antibody repertoire consistent with extensive secondary V(D)J recombination. Nevertheless,XLA B cells are enriched in autoreactive clones. Our results demonstrate that Btk is essential in regulating thresholds for human B cell tolerance. View Publication

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产品号#：

15024

15064

产品名：

RosetteSep™ 人B细胞富集抗体混合物

RosetteSep™人B细胞富集抗体混合物