搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

7:41

视频
Procedure for Setting Up the CFC Assay

发布日期: 9/21/10
产品手册
Worksheet for Proficiency Testing with Frozen Samples

产品类型：

品牌：

MethoCult

产品号#：

00602

00603

00608

00609

产品名：
产品手册
Worksheet for Proficiency Testing with Frozen Samples (French)

产品类型：

品牌：

MethoCult

产品号#：

00602

00603

00608

00609

产品名：
点播
Introduction to STEMvision for CFU Assay Scoring Course Learn how to standardize and automate the scoring of the CFU assay using STEMvision™.
10:25

视频
CFU Assay Instructions for Global Proficiency Testing Programs

发布日期: 2/14/17
技术公告
The Colony-Forming Cell (CFC) Assay for Cord Blood

产品类型：

细胞类型：

造血干/祖细胞

产品号#：

00217

00217UK

00215

00215UK

00215GER

产品名：
1:03:04

线上讲座
Optimizing CD34+ Cell Genome Editing for Efficiency and HSPC Maintenance

发布日期: 5/30/22
产品手册
Human Primary Cells: It All Starts with the Right Cells

产品类型：

品牌：

EasySep

产品号#：

70031

70032

70033

70034

70035

70035.1

70035.2

70036

70037

70039

70039.1

70039.2

70039.3

70039.4

70039.5

70039.6

70042

70046

70007

70007.1

70007.2

70094

70013

70013.1

70013.2

70014

70500

70500.1

70500.2

70501

70015

70016

70503

70503.1

70503.2

70503.3

70504

产品名：

冻存的人外周血CD4+CD45RO+ T细胞

冻存的人外周血CD19+CD27- Naïve B细胞

冻存的人外周血CD19+ B细胞

冻存的人外周血单核细胞

冻存的人外周血CD14+单核细胞

冻存的人外周血CD14+单核细胞

冻存的人外周血CD14+单核细胞

#N/A

冻存的人外周血CD56+细胞

冻存的人外周血巨噬细胞

冻存的人外周血浆细胞样树突状细胞

冻存的人脐带血单核细胞

冻存的人脐带血单核细胞

冻存的人脐带血单核细胞

#N/A

#N/A

#N/A

冻存的人脐带血Pan T细胞

#N/A

#N/A
技术公告
ALDHbr Assay Kit for Enumeration of HSPCs in Cord Blood by Flow Cytometry

产品类型：

细胞类型：

造血干/祖细胞

产品号#：

01711

产品名：

ALDHbr检测试剂盒
Yoo E-HE-S et al. (JAN 2003) Stem cells (Dayton,Ohio) 21 2 228--35

Adherent cells generated during long-term culture of human umbilical cord blood CD34+ cells have characteristics of endothelial cells and beneficial effect on cord blood ex vivo expansion.

Hematopoiesis depends on the association of hematopoietic stem cells with stromal cells that constitute the hematopoietic microenvironment. The in vitro development of the endothelial cell from umbilical cord blood (UCB) is not well established and has met very limited success. In this study,UCB CD34(+) cells were cultured for 5 weeks in a stroma-free liquid culture system using thrombopoietin,flt3 ligand,and granulocyte-colony stimulating factor. By week 4-5,we found that firmly adherent fibroblast-like cells were established. These cells showed characteristics of endothelial cells expressing von Willebrand factor,human vascular cell adhesion molecule-1,human intracellular adhesion molecule-1,human CD31,E-selectin,and human macrophage. Furthermore,when comparing an ex vivo system without an established endothelial monolayer to an ex vivo system with an established endothelial monolayer,better expansion of total nucleated cells,CD34(+) cells,and colony-forming units (CFUs)-granulocyte-macrophage and CFUs-granulocyte-erythroid-megakaryocyte-macrophage were found during culture. This phenomenon was in part due to the fact that a significant reduction of apoptotic fractions was found in the CD34(+) cells,which were cultured on the adherent monolayer for up to 5 weeks. To gather quantitative data on the number of endothelial cells derived from a given number of CD34 cells,we performed limiting dilution assay by using Poisson distribution: the number of tested cells (linear scale) producing a 37% negative culture (logarithmic scale) is the number of cells containing one endothelial cell. By this method,one endothelial cell may be found from 314 CD34(+) cells after 5 weeks of culture. These results suggest that the UCB CD34(+) cell fraction contains endothelial cell precursors,establishing the hematopoietic microenvironment and providing the beneficial effects through downregulating apoptosis on UCB expansion protocols. These observations may provide insight for future cellular therapy or graft engineering. View Publication

产品类型：

产品号#：

04434

04444

产品名：

MethoCult™H4434经典

MethoCult™H4434经典
Liu C et al. (DEC 2010) Blood 116 25 5518--27

Progenitor cell dose determines the pace and completeness of engraftment in a xenograft model for cord blood transplantation.

Two critical concerns in clinical cord blood transplantation are the initial time to engraftment and the subsequent restoration of immune function. These studies measured the impact of progenitor cell dose on both the pace and strength of hematopoietic reconstitution by transplanting nonobese diabetic/severe combined immunodeficiency/interleukin-2 receptor-gamma-null (NSγ) mice with lineage-depleted aldehyde dehydrogenase-bright CD34(+) human cord blood progenitors. The progress of each transplant was monitored over an extended time course by repeatedly analyzing the peripheral blood for human hematopoietic cells. In vivo human hematopoietic development was complete. After long-term transplantation assays (≥ 19 weeks),human T-cell development was documented within multiple tissues in 16 of 32 NSγ mice. Human T-cell differentiation was active within NSγ thymuses,as documented by the presence of CD4(+) CD8(+) T-cell progenitors as well as T-cell receptor excision circles. It is important to note that although myeloid and B-cell engraftment was detected as early as 4 weeks after transplantation,human T-cell development was exclusively late onset. High progenitor cell doses were associated with a robust human hematopoietic chimerism that accelerated both initial time to engraftment and subsequent T-cell development. At lower progenitor cell doses,the chimerism was weak and the human hematopoietic lineage development was frequently incomplete. View Publication

产品类型：

产品号#：

01700

01705

01701

01702

14056

14066

28600

19056

19056RF

19756

19756RF

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

ALDEFLUOR™测定缓冲液

L-Calc™有限稀释软件
技术公告
Identification of ALDH-Expressing Cancer Stem Cells Using ALDEFLUOR™

产品类型：

细胞类型：

乳腺细胞，造血干/祖细胞

产品号#：

00212

01700

01705

01701

01702

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

ALDEFLUOR™测定缓冲液