搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Alison MR et al. (DEC 2010) The Journal of pathology 222 4 335--44

Finding cancer stem cells: are aldehyde dehydrogenases fit for purpose?

Despite many years of intensive effort,there is surprisingly little consensus on the most suitable markers with which to locate and isolate stem cells from adult tissues. By comparison,the study of cancer stem cells is still in its infancy; so,unsurprisingly,there is great uncertainty as to the identity of these cells. Stem cell markers can be broadly categorized into molecular determinants of self-renewal,clonogenicity,multipotentiality,adherence to the niche,and longevity. This review assesses the utility of recognizing cancer stem cells by virtue of high expression of aldehyde dehydrogenases (ALDHs),probably significant determinants of cell survival through their ability to detoxify many potentially cytotoxic molecules,and contributing to drug resistance. Antibodies are available against the ALDH enzyme family,but the vast majority of studies have used cell sorting techniques to enrich for cells expressing these enzymes. Live cells expressing high ALDH activity are usually identified by the ALDEFLUOR kit and sorted by fluorescence activated cell sorting (FACS). For many human tumours,but notably breast cancer,cell selection based upon ALDH activity appears to be a useful marker for enriching for cells with tumour-initiating activity (presumed cancer stem cells) in immunodeficient mice,and indeed the frequency of so-called ALDH(bri) cells in many tumours can be an independent prognostic indicator. View Publication

产品类型：

产品号#：

01700

01705

01702

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

ALDEFLUOR™测定缓冲液

产品手册

ALDEFLUOR™: For Identification and Isolation of Viable Stem & Progenitor Cells

产品类型：

品牌：

ALDEFLUOR

产品号#：

01700

01702

01705

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™测定缓冲液

ALDEFLUOR™ DEAB试剂

科学海报

A Serum- and Bovine Pituitary Extract (BPE)-Free Medium Supporting Long-Term, Feeder-Free Expansion Of Primary Human Epidermal Keratinocytes that Retain Their Air-Liquid Interface Differentiation

产品类型：

Conference：

ISSCR 2021

产品号#：

产品名：

实验方案

How to Prepare Conditioned Medium for the Expansion of Epithelial Cells

产品类型：

研究方向：

上皮细胞生物学

产品号#：

产品名：

Chen J and Chen Z-L (MAR 2010) Chinese journal of cancer 29 3 265--9

Technology update for the sorting and identification of breast cancer stem cells.

Breast cancer stem cells are a group of undifferentiated cells with self-renewal and multidifferentiation potential. Chemotherapeutic and radiotherapeutic resistance,hypoxic resistance,high tumorigenicity,high cell invasion,and metastatic abilities are characteristics of these cells,which are responsible for breast cancer recurrence. Therefore,the correct sorting and identification of breast cancer stem cells is a primary step for research in this field. This article briefly describes the recent progress on sorting and identification technologies for breast cancer stem cells. Sorting technologies include the side population technique,technologies that depend on cell surface markers,ALDEFLUOR assays,and in situ detection. Identification technologies include mammosphere cultures,limited dilution in vitro,and in-vivo animal models. This review provides an important reference for breast cancer stem cell research,which will explore new methods for the treatment of patients with breast cancer. View Publication

产品类型：

产品号#：

01700

01705

01702

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

ALDEFLUOR™测定缓冲液

H. Cao et al. (JUN 2018) Human gene therapy 29 6 643--652

Transducing Airway Basal Cells with a Helper-Dependent Adenoviral Vector for Lung Gene Therapy.

A major challenge in developing gene-based therapies for airway diseases such as cystic fibrosis (CF) is sustaining therapeutic levels of transgene expression over time. This is largely due to airway epithelial cell turnover and the host immunogenicity to gene delivery vectors. Modern gene editing tools and delivery vehicles hold great potential for overcoming this challenge. There is currently not much known about how to deliver genes into airway stem cells,of which basal cells are the major type in human airways. In this study,helper-dependent adenoviral (HD-Ad) vectors were delivered to mouse and pig airways via intranasal delivery,and direct bronchoscopic instillation,respectively. Vector transduction was assessed by immunostaining of lung tissue sections,which revealed that airway basal cells of mice and pigs can be targeted in vivo. In addition,efficient transduction of primary human airway basal cells was verified with an HD-Ad vector expressing green fluorescent protein. Furthermore,we successfully delivered the human CFTR gene to airway basal cells from CF patients,and demonstrated restoration of CFTR channel activity following cell differentiation in air-liquid interface culture. Our results provide a strong rationale for utilizing HD-Ad vectors to target airway basal cells for permanent gene correction of genetic airway diseases. View Publication

产品类型：

产品号#：

05001

05021

05022

产品名：

PneumaCult™-ALI 培养基

PneumaCult™-ALI 培养基含12 mm Transwell®插件

PneumaCult™-ALI 培养基含6.5 mm Transwell®插件

Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope

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视频

Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope

发布日期: 04/18/2018

科学海报

Culture and Differentiation of Mouse Hepatic Organoids Using Serum-free HepatiCult™ Organoid Growth Medium

产品类型：

产品号#：

06030

06040

产品名：

HepatiCult™ 类器官生长培养基 (小鼠)

PancreaCult™ 类器官生长培养基 (小鼠)

产品手册

Reliable Antibodies for Your Research

产品类型：

品牌：

EasySep

产品号#：

60100

60100.1

60100AD

60100AD.1

60104

60104AD.1

60104AZ

60104AZ.1

60104BT

60104BT.1

60104FI.1

60104PE

60104PE.1

60106

60106.1

60106AZ

60106AZ.1

60106BT

60106FI

60106FI.1

60106PE

60106PE.1

产品名：

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

抗β-微管蛋白III抗体，clone AA10，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3

抗小鼠TCR Gamma/Delta抗体，clone GL3，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3，APC

抗小鼠TCR Gamma/Delta抗体，clone GL3，Biotin

抗小鼠TCR Gamma/Delta抗体，clone GL3，FITC

抗小鼠TCR Gamma/Delta抗体，clone GL3，PE

抗人CD71（转铁蛋白受体）抗体，clone OKT9

抗人CD71（转铁蛋白受体）抗体，clone OKT9，APC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，Biotin

抗人CD71（转铁蛋白受体）抗体，clone OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，PE

科学海报

A Reproducible Protocol for the Generation of Branching Lung Organoids From Human Pluripotent Stem Cells

产品类型：

Conference：

ISSCR 2021

产品号#：

产品名：

Eirew P et al. (DEC 2008) Nature medicine 14 12 1384--9

A method for quantifying normal human mammary epithelial stem cells with in vivo regenerative ability.

Previous studies have demonstrated that normal mouse mammary tissue contains a rare subset of mammary stem cells. We now describe a method for detecting an analogous subpopulation in normal human mammary tissue. Dissociated cells are suspended with fibroblasts in collagen gels,which are then implanted under the kidney capsule of hormone-treated immunodeficient mice. After 2-8 weeks,the gels contain bilayered mammary epithelial structures,including luminal and myoepithelial cells,their in vitro clonogenic progenitors and cells that produce similar structures in secondary transplants. The regenerated clonogenic progenitors provide an objective indicator of input mammary stem cell activity and allow the frequency and phenotype of these human mammary stem cells to be determined by limiting-dilution analysis. This new assay procedure sets the stage for investigations of mechanisms regulating normal human mammary stem cells (and possibly stem cells in other tissues) and their relationship to human cancer stem cell populations. View Publication

产品类型：

产品号#：

05601

产品名：

EpiCult™-B 人培养基

科学海报

HepatiCult™ for Human Hepatic Organoids: A Validated Culture System for Drug Toxicity Screening

产品类型：

产品号#：

产品名：

搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Finding cancer stem cells: are aldehyde dehydrogenases fit for purpose?

Technology update for the sorting and identification of breast cancer stem cells.

Transducing Airway Basal Cells with a Helper-Dependent Adenoviral Vector for Lung Gene Therapy.

A method for quantifying normal human mammary epithelial stem cells with in vivo regenerative ability.

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