搜索结果: 'stemprep kits'

技术手册
Enzymatic Dissociation of Adult Mouse and Rat CNS Tissue Using the NeuroCult™ Enzymatic Dissociation Kit

产品类型：

Cell Culture Media and Supplements

产品号#：

05715

产品名：

NeuroCult™成年中枢神经系统（CNS）组织酶解试剂盒（小鼠和大鼠）
技术手册
Chemical Dissociation of Neurospheres Derived from Embryonic and Adult Mouse CNS using the NeuroCult™ Chemical Dissociation Kit

产品类型：

Tissue and Cell Culture Dissociation Reagents

产品号#：

05707

产品名：

NeuroCult™化学解离试剂盒（小鼠）
技术手册
Identification and Enumeration of Mouse Neural Stem and Progenitor Cells With the NeuroCult™ Neural Colony-Forming Cell (NCFC) Assay Kit

产品类型：

Cell Culture Media and Supplements

产品号#：

05740

产品名：
技术手册
Identification and Enumeration of Rat Neural Stem and Progenitor Cells With the NeuroCult™ Neural Colony-Forming Cell (NCFC) Assay Kit

产品类型：

Cell Culture Media and Supplements

产品号#：

05742

产品名：
文献

Goldstein BJ et al. (DEC 2016) Development 143 23 4394--4404

Contribution of Polycomb group proteins to olfactory basal stem cell self-renewal in a novel c-KIT+ culture model and in vivo.

Olfactory epithelium (OE) has a lifelong capacity for neurogenesis due to the presence of basal stem cells. Despite the ability to generate short-term cultures,the successful in vitro expansion of purified stem cells from adult OE has not been reported. We sought to establish expansion-competent OE stem cell cultures to facilitate further study of the mechanisms and cell populations important in OE renewal. Successful cultures were prepared using adult mouse basal cells selected for expression of c-KIT. We show that c-KIT signaling regulates self-renewal capacity and prevents neurodifferentiation in culture. Inhibition of TGFβ family signaling,a known negative regulator of embryonic basal cells,is also necessary for maintenance of the proliferative,undifferentiated state in vitro Characterizing successful cultures,we identified expression of BMI1 and other Polycomb proteins not previously identified in olfactory basal cells but known to be essential for self-renewal in other stem cell populations. Inducible fate mapping demonstrates that BMI1 is expressed in vivo by multipotent OE progenitors,validating our culture model. These findings provide mechanistic insights into the renewal and potency of olfactory stem cells. View Publication

产品类型：

产品号#：

05700

产品名：

NeuroCult™基础培养基（小鼠和大鼠）