搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

How to Optimize Your T Cell Therapy Workflow—Without the Use of Serum or Feeder Cells In this talk, Dr. Dominika Nackiewicz introduces how STEMCELL Technologies can help optimize your T cell cultures with robust, high-performance products and expert support. You will learn how to generate high yields of functional T cells for cell therapy development and manufacturing using the new GMP ImmunoCult™ medium and activators. She will also discuss efficient gene editing strategies using the new CellPore™ Transfection System and solutions for T cell generation from pluripotent stem cells or hematopoietic stem and progenitor cells.

This webinar is part of the “Translational T Cell Talks: Scaling for the Future” event hosted in collaboration with Scientist.com on June 11, 2024. View the rest of the talks and read the Q&A transcripts.

Advances in Point-of-Care Cell Engineering—What Are You Feeding Your CAR T Cells? In this webinar, Dr. Julian J. Lum discusses key factors to consider when choosing metabolic assays and how they can be used to assess cell function. Additionally, he discusses the metabolic characteristics of different commercially available cell culture media formulations as well as the importance of assessing the array of media products and their impact on metabolism.

This webinar is part of the “Translational T Cell Talks: Scaling for the Future” event hosted in collaboration with Scientist.com on June 11, 2024. View the rest of the talks and read the Q&A transcripts.

Comparison of Mouse Immune Cell Isolation Protocols: EasySep™ vs. Column-Based Magnetic Separation A head-to-head, in-lab comparison of two mouse immune cell isolation protocols: the 15-minute EasySep™ cell isolation kit and a traditional column-based cell isolation kit

How to Prepare a Single-Cell Suspension from Primary Tissue Samples (e.g. Mouse Spleen) How to harvest cells from a mouse spleen and prepare a single cell suspension prior to performing cell isolation to enable cell separation by minimizing additional cell loss and improving the labeling of target cells

Tools for Optimizing Human Immune Cell Research Obtaining consistent and reliable results when culturing immune cells can be challenging. Watch this webinar to discover how to obtain high yields of functional T cells, NK cells, B cells, dendritic cells, and macrophages for your research applications. The speaker, Evan Karas, also explains how to expand primary T cells without feeders or serum.

How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology Isolation of human CD4+ T cells by negative selection using EasySep™ to separate highly purified cells in as little as 8 minutes

How To Isolate Cells In 25 Minutes With EasySep™ Isolate untouched cells in just 25 minutes, using the EasySep™ column-free cell isolation system

How to Count Cells Using a Hemocytometer How to prepare and count stained cell samples using a hemocytometer for both total nucleated cell counts and viable cell counts

Isolate Cells From Leuko Paks Without Density Centrifugation Preparing Leuko Paks for cell isolation without density centrifugation

How to Isolate Nucleated Cells from Whole Blood Using HetaSep™ Erythrocyte Aggregation Agent HetaSep™ is an erythrocyte aggregation agent used to quickly separate nucleated cells from red blood cells

How to Prepare a Single Cell Suspension from Frozen Samples and Prevent Cell Clumping Learn how to harvest cells from a frozen starting sample and prepare a single cell suspension prior to performing cell isolation

Non-Viral CRISPR Knock-In Anti-B7-H3 CAR-T Cells Are Amenable for Treatment of Subtypes of Small Cell Lung Cancer In this webinar, Scientist Vimal Keerthi discusses his work on identifying CD276 (B7-H3) overexpression in primary human SCLC and developing a non-viral CRISPR-Cas9 knock-in (CKI) based platform against B7-H3 to manufacture CAR T cells for the treatment of SCLC. He demonstrates the feasibility of this CAR T manufacturing platform and how this provides a blueprint for immediate clinical translation, overcoming the bottleneck of viral vector production.

This webinar is part of the “Translational T Cell Talks: Scaling for the Future” event hosted in collaboration with Scientist.com on June 11, 2024. View the rest of the talks and read the Q&A transcripts.