Development of Simplified and Defined Cell Culture for Human Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. TeSR™-E8™ is a defined, xeno-free medium with a simple and published formula that was developed to meet the demands of a wide range of applications. The E8 culture system has been successfully used to derive and maintain hPSCs, and enzyme-free methods have also been developed for cell expansion and cryopreservation. More recently, defined differentiation conditions have also been established for specific lineages. These developments will provide an efficient and cost-effective E8 platform for the hPSC field. This webinar is presented by Dr. Guokai Chen at the University of Macau.
Highly Characterized Human iPSCs and NPCs for Downstream Differentiation Applications
Reproducible research with human induced pluripotent stem cells (iPSCs) depends on access to thoroughly characterized and quality-controlled cell lines. In this webinar, Drs. Andrew Gaffney and Erin Knock will describe the generation of a standardized induced pluripotent stem cell (iPSC) line. Developed with the upcoming ISSCR Standards Initiative characterization guidelines in mind, this highly characterized line is karyotypically stable, demonstrates trilineage differentiation potential, and expresses undifferentiated cell markers. Further, STEMCELL has developed a highly pure, ready-to-use neural progenitor cell product expressing PAX6 and SOX1 over multiple passages. Dr. Knock will show how these multipotent cells are suitable for customized downstream differentiation to various CNS cell types, such as forebrain neurons, midbrain neurons, and astrocytes. These progenitor cells are the ideal controls for standardizing downstream differentiation protocols, modeling diseases, and developing assays.
Madeline Lancaster on Brain Organoids: Modeling Human Brain Development in a Dish
In this webinar, Dr. Madeline Lancaster, who has done groundbreaking work in the development of cerebral organoid technology, discusses the latest developments in cerebral organoids and provides a comparison of different 3D model systems being used for neurological research.
Research in the Lancaster lab focuses on human brain development using cerebral organoids, a new in vitro model system for neurological development and disease research. The laboratory uses these ‘mini-brains’ to study the most fundamental differences between human and other mammalian species’ brain development - essentially, what makes us human. The lab is also studying cellular mechanisms of, and potential therapeutic avenues for, neurodevelopmental disorders such as autism and intellectual disability.
This webinar is just one of the educational resources on neural organoids that we’ve developed to help you navigate this exciting field. Visit the Neural Organoid Information Hub to learn more.