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Implementing the Colony-Forming Unit (CFU) Assay As a Potency Assay for Hematopoietic Cell Therapy Products Assessing the functional potency of cells for use in cell therapy research presents unique challenges. For hematopoietic stem and progenitor cells (HSPCs) in particular, potency can be measured in vitro by assessing the ability of these cells to differentiate into progenitor cells using the colony-forming unit (CFU) assay. Validating the CFU assay as a potency assay requires demonstrating its specificity, accuracy, precision, linearity, and reproducibility. Once validated, the CFU assay can assess the quality and consistency of prospective hematopoietic cell therapy products (HCTPs) at multiple stages of the processing and manufacturing workflow.

Join Dr. Colin Hammond and learn about the regulatory guidance around the potency testing of HCTPs and how to validate the CFU assay as a potency assay that can be integrated into cell therapy manufacturing workflows.

Optimization and Standardization of the Colony-Forming Unit Assay for Hematopoietic Progenitor Cells

EasySep™ Mouse Cell Isolation Kits: How Much Time Will You Save? What are immunologists doing with all the time they're saving with STEMCELL Technologies' 15-minute EasySep™ mouse cell isolation kits? How much time will you save?

How to Prepare a Single-Cell Suspension from Primary Tissue Samples (e.g. Mouse Spleen) How to harvest cells from a mouse spleen and prepare a single cell suspension prior to performing cell isolation to enable cell separation by minimizing additional cell loss and improving the labeling of target cells

The cytokine-inducible Scr homology domain-containing protein negatively regulates signaling by promoting apoptosis in erythroid progenitor cells.

Expansion and Differentiation of Hematopoietic Stem and Progenitor Cells into Natural Killer (NK) Cells Using StemSpan™ Medium and Supplements

MesenPure™: For Highly Enriched Mouse MSC Cultures The MesenCult™ Proliferation Kit with MesenPure™ (Mouse) has been optimized for the enrichment and expansion of mouse bone marrow- and compact bone-derived mesenchymal stem cells (MSCs) as early as passage 0

Pluripotent Stem Cells Few areas of biology currently garner more attention than the study of human pluripotent stem cells (hPSCs)

Robust Scale-Up of Human Pluripotent Stem Cells Using TeSR™ 3D Media

Development of a liposomal nanoparticle formulation of 5-fluorouracil for parenteral administration: formulation design, pharmacokinetics and efficacy.

EasySep™ Mouse Cell Isolation Kits: How Much Time Will You Save? (Part 2) What are immunologists doing with all the time they're saving with STEMCELL Technologies' 15-minute EasySep™ mouse cell isolation kits? In part 2, Meghan learns yoga. How much time will you save?

CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells