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A Guide to Passaging Human Pluripotent Stem Cells Using mTeSR™1 A guide to passaging human pluripotent stem cells using mTeSR™1

Daniel Gray, PhD Dr. Daniel Gray describes his work studying cell death and the potential applications in drug development for immune disorders

“Dendritic Cells and the Immune System” Featuring Dr. Caetano Reis e Sousa On this episode of the Immunology Podcast, Dr. Caetano Reis e Sousa talks about the mechanisms of dendritic cell cross-presentation, RNA interference, and dendritic cell progenitors in influenza.

How to Isolate Cells in 96-Well Plates Using the EasyPlate™ Cell Separation Magnet Learn how to isolate highly purified, untouched cells in as little as 25 minutes in a convenient format for downstream analysis

How to Prepare a Single Cell Suspension from Frozen Samples and Prevent Cell Clumping Learn how to harvest cells from a frozen starting sample and prepare a single cell suspension prior to performing cell isolation

Reducing Platelet Contamination in Blood Samples Platelets (thrombocytes), cell fragments derived from megakaryocytes, are used in normal blood clotting, and must be handled gently to prevent activation

Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells Dr. Peter Coffey from University College London shares his work with the London Project to Cure Blindness, which resulted in the first clinical stem cell trial to treat blindness, and discusses cell quality requirements for clinical trials. This presentation and the following Q&A session were moderated by Dr. Christine Mummery from the Leiden University Medical Center.

This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.

Note: Some original data from this presentation has been omitted to abide by copyright rules.

Supplementary Protocols for IntestiCult™ Organoid Growth Medium (Human) Protocols for the differentiation of human intestinal organoids, growth as a monolayer culture, and cryopreservation and thawing protocols for long-term storage.

Scaling Biomimetic Culture of Pluripotent Stem Cells Hear from Dr. Maxime Feyeux, who discusses what the in vivo growth environment of pluripotent stem cells (PSCs) can teach us about in vitro culture. He summarizes some of the current challenges facing standard PSC culture technologies and shares a solution for mass producing high-quality PSCs for downstream applications.

Air-Liquid Interface Culture for Respiratory Research .

Non-Viral CRISPR Knock-In Anti-B7-H3 CAR-T Cells Are Amenable for Treatment of Subtypes of Small Cell Lung Cancer In this webinar, Scientist Vimal Keerthi discusses his work on identifying CD276 (B7-H3) overexpression in primary human SCLC and developing a non-viral CRISPR-Cas9 knock-in (CKI) based platform against B7-H3 to manufacture CAR T cells for the treatment of SCLC. He demonstrates the feasibility of this CAR T manufacturing platform and how this provides a blueprint for immediate clinical translation, overcoming the bottleneck of viral vector production.

This webinar is part of the “Translational T Cell Talks: Scaling for the Future” event hosted in collaboration with Scientist.com on June 11, 2024. View the rest of the talks and read the Q&A transcripts.

Natural Killer Cells Overview of NK cell receptors, subsets, activation and function