搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'
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MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation Use MesenCult™ to derive, culture, expand, differentiate, and cryopreserve MSCs
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New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells The development of standardized ex vivo HSPC expansion methods is needed to generate clinically relevant cell numbers for transplantation. This webinar will review key concepts in human HSPC biology, including standardized methods and recently demonstrated strategies for expanding HSPCs in culture. Additionally, STEMCELL products for the entire workflow of HSPC expansion will be covered, from isolation to expansion and differentiation, and analysis.
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Assays for Hematopoietic Stem and Progenitor Cells in Cord Blood This webinar, by Dr. Bert Wognum, will provide an overview of the value of assessing hematopoietic progenitor cell content in cord blood. In addition to reviewing the current assays used, new methods incorporating automation will be discussed.
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Human Hematopoietic Stem and Progenitor Cell Phenotyping Overview of subset surface markers, frequencies and assays for analysis
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Air-Liquid Interface (ALI) Culture of Bronchial Epithelial Cells with PneumaCult™-ALI Medium PneumaCult™-ALI is a serum- and BPE-free medium for the culture of primary human bronchial epithelial cells (HBECs) at the ALI
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Implementing the Colony-Forming Unit (CFU) Assay As a Potency Assay for Hematopoietic Cell Therapy Products Assessing the functional potency of cells for use in cell therapy research presents unique challenges. For hematopoietic stem and progenitor cells (HSPCs) in particular, potency can be measured in vitro by assessing the ability of these cells to differentiate into progenitor cells using the colony-forming unit (CFU) assay. Validating the CFU assay as a potency assay requires demonstrating its specificity, accuracy, precision, linearity, and reproducibility. Once validated, the CFU assay can assess the quality and consistency of prospective hematopoietic cell therapy products (HCTPs) at multiple stages of the processing and manufacturing workflow.
Join Dr. Colin Hammond and learn about the regulatory guidance around the potency testing of HCTPs and how to validate the CFU assay as a potency assay that can be integrated into cell therapy manufacturing workflows.