搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Tan J et al. (MAY 2007) Genes & development 21 9 1050--63

Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells.

Polycomb-repressive complex 2 (PRC2)-mediated histone methylation plays an important role in aberrant cancer gene silencing and is a potential target for cancer therapy. Here we show that S-adenosylhomocysteine hydrolase inhibitor 3-Deazaneplanocin A (DZNep) induces efficient apoptotic cell death in cancer cells but not in normal cells. We found that DZNep effectively depleted cellular levels of PRC2 components EZH2,SUZ12,and EED and inhibited associated histone H3 Lys 27 methylation (but not H3 Lys 9 methylation). By integrating RNA interference (RNAi),genome-wide expression analysis,and chromatin immunoprecipitation (ChIP) studies,we have identified a prominent set of genes selectively repressed by PRC2 in breast cancer that can be reactivated by DZNep. We further demonstrate that the preferential reactivation of a set of these genes by DZNep,including a novel apoptosis affector,FBXO32,contributes to DZNep-induced apoptosis in breast cancer cells. Our results demonstrate the unique feature of DZNep as a novel chromatin remodeling compound and suggest that pharmacologic reversal of PRC2-mediated gene repression by DZNep may constitute a novel approach for cancer therapy. View Publication

产品类型：

产品号#：

72322

72324

产品名：

3-Deazaneplanocin A

(Jun 2025) Nature Neuroscience 28 6

Neuronal aging causes mislocalization of splicing proteins and unchecked cellular stress

Aging is one of the most prominent risk factors for neurodegeneration,yet the molecular mechanisms underlying the deterioration of old neurons are mostly unknown. To efficiently study neurodegeneration in the context of aging,we transdifferentiated primary human fibroblasts from aged healthy donors directly into neurons,which retained their aging hallmarks,and we verified key findings in aged human and mouse brain tissue. Here we show that aged neurons are broadly depleted of RNA-binding proteins,especially spliceosome components. Intriguingly,splicing proteins—like the dementia- and ALS-associated protein TDP-43—mislocalize to the cytoplasm in aged neurons,which leads to widespread alternative splicing. Cytoplasmic spliceosome components are typically recruited to stress granules,but aged neurons suffer from chronic cellular stress that prevents this sequestration. We link chronic stress to the malfunctioning ubiquitylation machinery,poor HSP90? chaperone activity and the failure to respond to new stress events. Together,our data demonstrate that aging-linked deterioration of RNA biology is a key driver of poor resiliency in aged neurons. Rhine et al. find that neuronal aging leads to widespread dysregulation of RNA biology,including mislocalization of splicing proteins like TDP-43,chronic cellular stress and reduced resiliency. View Publication

产品类型：

产品号#：

100-0276

100-1130

产品名：

mTeSR™ Plus

Brazel CY et al. (AUG 2005) Aging cell 4 4 197--207

Sox2 expression defines a heterogeneous population of neurosphere-forming cells in the adult murine brain.

The identification of neural stem cells (NSCs) in situ has been prevented by the inability to identify a marker consistently expressed in all adult NSCs and is thus generally accomplished using the in vitro neurosphere-forming assay. The high-mobility group transcription factor Sox2 is expressed in embryonic neural epithelial stem cells; because these cells are thought to give rise to the adult NSC population,we hypothesized that Sox2 may continue to be expressed in adult NSCs. Using Sox2:EGFP transgenic mice,we show that Sox2 is expressed in neurogenic regions along the rostral-caudal axis of the central nervous system throughout life. Furthermore,all neurospheres derived from these neurogenic regions express Sox2,suggesting that Sox2 is indeed expressed in adult NSCs. We demonstrate that NSCs are heterogeneous within the adult brain,with differing capacities for cell production. In vitro,all neurospheres express Sox2,but the expression of markers common to early progenitor cells within individual neurospheres varies; this heterogeneity of NSCs is mirrored in vivo. For example,both glial fibrillary acidic protein and NG2 are expressed within individual neurospheres,but their expression is mutually exclusive; likewise,these two markers show distinct staining patterns within the Sox2+ regions of the brain's neurogenic regions. Thus,we propose that the expression of Sox2 is a unifying characteristic of NSCs in the adult brain,but that not all NSCs maintain the ability to form all neural cell types in vivo. View Publication

产品类型：

产品号#：

05700

05701

05702

产品名：

NeuroCult™ 基础培养基(小鼠&大鼠)

NeuroCult™ 扩增添加物 (小鼠&大鼠)

NeuroCult™ 扩增试剂盒 (小鼠&大鼠)

产品手册

Antibodies for Cell Culture

产品类型：

品牌：

EasySep

产品号#：

60100

60100.1

60100AD

60100AD.1

60108

60108AZ

60108FI

60108PE

60108PS

60089

60089AZ

60089AZ.1

60089BT

60089PE

60089PE.1

60091

60091.1

60091AD

60091AD.1

60072

60072.1

60072AD

60072AZ

60072AZ.1

60072BT

60072BT.1

60072FI

60072FI.1

60072PE

60072PE.1

60072PS

6

产品名：

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

抗β-微管蛋白III抗体，clone AA10，Alexa Fluor® 488

小鼠IgG2a，kappa同型对照抗体，克隆C1.18.4

小鼠IgG2a，kappa同型对照抗体，clone C1.18.4，PE

抗人CD184（CXCR4）抗体，克隆12G5

抗人CD184（CXCR4）抗体，克隆12G5，APC

抗人CD184（CXCR4）抗体，clone 12G5，Biotin

抗人CD184（CXCR4）抗体，克隆12G5，PE

抗人巢蛋白抗体，克隆10C2

抗人巢蛋白抗体，clone 10C2，Alexa Fluor® 488

抗人巢蛋白抗体，克隆10C2，Alexa Fluor® 488

小鼠IgG2b，kappa同型对照抗体（clone MPC-11）

小鼠IgG2b，kappa同型对照抗体（clone MPC-11），Alexa Fluor® 488

小鼠IgG2b，kappa同型对照抗体（clone MPC-11），APC

小鼠IgG2b，kappa同型对照抗体（clone MPC-11），Biotin

小鼠IgG2b，kappa同型对照抗体（clone MPC-11），FITC

小鼠IgG2b，kappa同型对照抗体（clone MPC-11），PE

Event

Austrian Society for Immunology

产品类型：

产品号#：

产品名：

科学海报

Immunomagnetic Cell Enrichment of Lymphoid Progenitors from Mouse Bone Marrow

产品类型：

Conference：

ISEH 2011

产品号#：

18000

产品名：

EasySep™磁极

科学海报

Animal Component- and Extracellular Vesicle-Free Medium Facilitates Isolation and Characterization of Extracellular Vesicles from Human Bone Marrow Mesenchymal Stromal Cells

产品类型：

Conference：

ISCT 2025

产品号#：

产品名：

D. Kobayashi et al. ( 2022) Frontiers in immunology 13 973880

Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway.

Type-2 bitter taste receptors (Tas2Rs) are a large family of G protein-coupled receptors that are expressed in the oral cavity and serve to detect substances with bitter tastes in foods and medicines. Recent evidence suggests that Tas2Rs are also expressed extraorally,including in immune cells. However,the role of Tas2Rs in immune cells remains controversial. Here,we demonstrate that Tas2R126,Tas2R135,and Tas2R143 are expressed in mouse neutrophils,but not in other immune cells such as macrophages or T and B lymphocytes. Treatment of bone marrow-derived neutrophils from wild-type mice with the Tas2R126/143 agonists arbutin and d-salicin led to enhanced C-X-C motif chemokine ligand 2 (CXCL2)-stimulated migration in vitro,but this response was not observed in neutrophils from Tas2r126/135/143-deficient mice. Enhancement of CXCL2-stimulated migration by Tas2R agonists was accompanied by increased phosphorylation of myosin light chain 2 (MLC2) and was blocked by pretreatment of neutrophils with inhibitors of Rho-associated coiled-coil-containing protein kinase (ROCK),but not by inhibitors of the small GTPase RhoA. Taken together,these results demonstrate that mouse neutrophils express functional Tas2R126/143 and suggest a role for Tas2R126/143-ROCK-MLC2-dependent signaling in the regulation of neutrophil migration. View Publication

产品类型：

产品号#：

19762

产品名：

EasySep™小鼠中性粒细胞富集试剂盒

A. Reuter et al. ( 2015) The Journal of Immunology 194 2696-2705

Criteria for Dendritic Cell Receptor Selection for Efficient Antibody-Targeted Vaccination

Ab-targeted vaccination involves targeting a receptor of choice expressed by dendritic cells (DCs) with Ag-coupled Abs. Currently,there is little consensus as to which criteria determine receptor selection to ensure superior Ag presentation and immunity. In this study,we investigated parameters of DC receptor internalization and determined how they impact Ag presentation outcomes. First,using mixed bone marrow chimeras,we established that Ag-targeted,but not nontargeted,DCs are responsible for Ag presentation in settings of Ab-targeted vaccination in vivo. Next,we analyzed parameters of DEC205 (CD205),Clec9A,CD11c,CD11b,and CD40 endocytosis and obtained quantitative measurements of internalization speed,surface turnover,and delivered Ag load. Exploiting these parameters in MHC class I (MHC I) and MHC class II (MHC II) Ag presentation assays,we showed that receptor expression level,proportion of surface turnover,or speed of receptor internalization did not impact MHC I or MHC II Ag presentation efficiency. Furthermore,the Ag load delivered to DCs did not correlate with the efficiency of MHC I or MHC II Ag presentation. In contrast,targeting Ag to CD8(+) or CD8(-) DCs enhanced MHC I or MHC II Ag presentation,respectively. Therefore,receptor expression levels,speed of internalization,and/or the amount of Ag delivered can be excluded as major determinants that dictate Ag presentation efficiency in setting of Ab-targeted vaccination. View Publication

产品类型：

产品号#：

19251

产品名：

EasySep™人Pan-DC预富集试剂盒

科学海报

Rapid Unbiased Enrichment (Negative Selection) of Circulating Non-Hematopoietic Tumor Cells Directly from Whole Blood

产品类型：

Conference：

AACR 2012

产品号#：

产品名：

Cantu' C et al. (JAN 2011) Nucleic acids research 39 2 486--501

A highly conserved SOX6 double binding site mediates SOX6 gene downregulation in erythroid cells.

The Sox6 transcription factor plays critical roles in various cell types,including erythroid cells. Sox6-deficient mice are anemic due to impaired red cell maturation and show inappropriate globin gene expression in definitive erythrocytes. To identify new Sox6 target genes in erythroid cells,we used the known repressive double Sox6 consensus within the εy-globin promoter to perform a bioinformatic genome-wide search for similar,evolutionarily conserved motifs located within genes whose expression changes during erythropoiesis. We found a highly conserved Sox6 consensus within the Sox6 human gene promoter itself. This sequence is bound by Sox6 in vitro and in vivo,and mediates transcriptional repression in transient transfections in human erythroleukemic K562 cells and in primary erythroblasts. The binding of a lentiviral transduced Sox6FLAG protein to the endogenous Sox6 promoter is accompanied,in erythroid cells,by strong downregulation of the endogenous Sox6 transcript and by decreased in vivo chromatin accessibility of this region to the PstI restriction enzyme. These observations suggest that the negative Sox6 autoregulation,mediated by the double Sox6 binding site within its own promoter,may be relevant to control the Sox6 transcriptional downregulation that we observe in human erythroid cultures and in mouse bone marrow cells in late erythroid maturation. View Publication

产品类型：

产品号#：

09600

09650

09850

产品名：

StemSpan™ SFEM

Ma N et al. (NOV 2013) Journal of Biological Chemistry 288 48 34671--34679

Transcription activator-like effector nuclease (TALEN)-mediated Gene correction in integration-free β-Thalassemia induced pluripotent stem cells

$\$-Thalassemia ($\$-Thal) is a group of life-threatening blood disorders caused by either point mutations or deletions of nucleotides in $\$-globin gene (HBB). It is estimated that 4.5% of the population in the world carry $\$-Thal mutants (1),posing a persistent threat to public health. The generation of patient-specific induced pluripotent stem cells (iPSCs) and subsequent correction of the disease-causing mutations offer an ideal therapeutic solution to this problem. However,homologous recombination-based gene correction in human iPSCs remains largely inefficient. Here,we describe a robust process combining efficient generation of integration-free $\$-Thal iPSCs from the cells of patients and transcription activator-like effector nuclease (TALEN)-based universal correction of HBB mutations in situ. We generated integration-free and gene-corrected iPSC lines from two patients carrying different types of homozygous mutations and showed that these iPSCs are pluripotent and have normal karyotype. We showed that the correction process did not generate TALEN-induced off targeting mutations by sequencing. More importantly,the gene-corrected $\$-Thal iPS cell lines from each patient can be induced to differentiate into hematopoietic progenitor cells and then further to erythroblasts expressing normal $\$-globin. Our studies provide an efficient and universal strategy to correct different types of $\$-globin mutations in $\$-Thal iPSCs for disease modeling and applications. View Publication

产品类型：

产品号#：

05850

05857

05870

05875

85850

85857

85870

85875

产品名：

mTeSR™1

搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells.

Neuronal aging causes mislocalization of splicing proteins and unchecked cellular stress

Sox2 expression defines a heterogeneous population of neurosphere-forming cells in the adult murine brain.

Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway.

Criteria for Dendritic Cell Receptor Selection for Efficient Antibody-Targeted Vaccination

A highly conserved SOX6 double binding site mediates SOX6 gene downregulation in erythroid cells.

Transcription activator-like effector nuclease (TALEN)-mediated Gene correction in integration-free β-Thalassemia induced pluripotent stem cells

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