搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

L. Truszkowski et al. (Sep 2025) Open Research Europe 4 2

Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture

Cost-effective,practical,and reproducible culture of human pluripotent stem cells (hPSCs) is required for basic and translational research. Basal 8 (B8) has emerged as a cost-effective solution for weekend-free and chemically-defined hPSC culture. However,the requirement to home-produce some recombinant growth factors for B8 can hinder access and reproducibility. Moreover,we found the published B8 formulation suboptimal in widely-used normoxic hPSC culture. Lastly,the performance of B8 in functional applications such as genome editing or organoid differentiation required systematic evaluation. We formulated B8 with commercially available,growth factors and adjusted its composition to support normoxic culture of WTC11 human induced pluripotent stem cell line. We compared this formulation (B8+) with commercial Essential 8 (cE8) and a home-made,weekend-free E8 formulation (hE8). We measured pluripotency marker expression and cell cycle by flow cytometry,and investigated the transcriptional profiles by bulk and single-cell RNA sequencing. We further assessed genomic stability,genome editing efficiency,single-cell cloning,and differentiation in both monolayer and organoids. Finally,we validated key findings using male (H1) and female (H9) human embryonic stem cells. hE8 performed comparably to cE8 across most functional assays and cell lines. In contrast,cells in B8+ displayed higher NANOG expression and improved genome editing efficiency. At the same time,B8+ led to gene expression changes indicative of marked lineage priming,reflected in altered morphology and differential response to some differentiation protocols. Both weekend-free media resulted in a modest transcriptional shift towards a less metabolically active state,consistent with intermittent media starvation. Homemade weekend-free media can provide a cost-effective alternative to commercial formulations. hE8,integrating some features of B8 while resembling cE8,emerges as a robust and practical option with limited compromises. B8+,though advantageous in some contexts,warrants caution due to lineage priming effects that may impact differentiation outcomes. View Publication

产品类型：

产品号#：

05230

产品名：

STEMdiff™ 三胚层分化试剂盒

科学海报

Generation of T Cells from Hematopoietic Stem and Progenitor Cells in a Stroma-Free, Serum-Free Culture System

产品类型：

产品号#：

09940

09605

09915

09925

09930

产品名：

StemSpan™ T细胞生成试剂盒

StemSpan™ SFEM II

StemSpan™淋系祖细胞扩增添加物（10X）

StemSpan™淋系祖细胞分化包板材料（100X）

StemSpan™ T细胞祖细胞成熟添加物（10X）

Sammar M et al. (JUL 1994) International immunology 6 7 1027--36

Heat-stable antigen (CD24) as ligand for mouse P-selectin.

Heat-stable antigen (HSA)/CD24 is a cell surface molecule expressed by many cell types in the mouse. The molecule has an unusual structure because of its small protein core and extensive glycosylation. In order to study the functional role of the HSA-associated glycoconjugates we have isolated different forms of HSA. Using lectin analysis we provide evidence for extensive heterogeneity in carbohydrate composition and sialic acid linkage. Several HSA forms were recognized by mouse P-selectin-IgG but not E-selectin-IgG in ELISA. As expected,P-selectin-IgG also bound to L2/HNK-1-positive neural glycoproteins (L2-glycoproteins) and sulfatides but not to gangliosides and other control glycoproteins. The binding of P-selectin-IgG to L2-glycoproteins and HSA required bivalent cations. The reactivity to HSA was sensitive to sialidase treatment whereas the binding to L2-glycoproteins was not. Studies with alpha 2-6 sialytransferase indicated that alpha 2-6 linked sialic acid was not involved in the P-selectin binding to HSA. Surprisingly,an L2/HNK-1 specific antibody was found to cross-react with some HSA glycoforms and its binding correlated with P-selectin-IgG reactivity. L2/HNK-1-positive or L2/HNK-1-negative HSA glycoforms were also analyzed after coating to polystyrene beads. Only the L2/HNK-1-positive HSA coated beads were reactive with P-selectin-IgG and could bind to activated bend3 endothelioma cells expressing P-selectin whereas the L2/HNK-1-negative HSA beads did not. It is suggested that in its L2/HNK-1 modified form the HSA molecule on leukocytes could represent a ligand for P-selectin on endothelial cells or platelets. View Publication

产品类型：

产品号#：

01434

产品名：

Wognum AW et al. (OCT 1990) Blood 76 7 1323--9

A specific in vitro bioassay for measuring erythropoietin levels in human serum and plasma.

The accurate measurement of biologically active erythropoietin (Ep) in human serum and plasma using present in vivo and in vitro bioassays is difficult because of the presence of both inhibitors and non-Ep stimulators of erythropoiesis. We have developed a simple procedure to quantitatively purify Ep from serum and plasma for subsequent testing in the phenylhydrazine-treated mouse spleen cell assay. The method involves absorption of Ep to an immobilized high-affinity anti-Ep monoclonal antibody and acid elution of the antibody-bound material. After neutralization,the eluted EP is then tested directly in the in vitro bioassay without interference by other serum proteins. By using magnetic beads as a solid support for the antibody,washing and elution steps can be performed rapidly and efficiently. Recoveries of Ep after this procedure show very little sample-to-sample variation and are consistently between 45% and 55%,which is close to the maximum binding expected for the anti-Ep antibody. Coupled with the 7.4-fold concentration that this procedure affords,there is an overall increase in sensitivity of three- to fourfold,which makes this assay suitable for accurately measuring Ep levels in patients with below-average titers. Results with this magnetic bead assay indicate that accurate and reproducible estimates for Ep levels in the serum and plasma from healthy donors as well as from patients with hematologic disorders can be obtained. Titers of biologically active Ep in the sera from a group of patients with either leukemia or lymphoma were found to be elevated,and the values correlated well with titers of immunoreactive Ep measured in the Ep radioimmunoassay. Because of its specificity and high sensitivity,the magnetic bead assay is a valuable alternative to immunoassays for the measurement of elevated,normal,and even subnormal Ep levels in human serum and plasma. View Publication

产品类型：

产品号#：

01630

产品名：

促红细胞生成素（EPO） ELISA试剂盒

实验方案

How to Isolate Mouse Peritoneal Cells

产品类型：

研究方向：

免疫学

产品号#：

产品名：

How to Count Intestinal Crypts for Mouse Organoid Cultures

1:09

视频

How to Count Intestinal Crypts for Mouse Organoid Cultures

发布日期: 04/23/2019

Bruserud O et al. (DEC 2000) Journal of hematotherapy & stem cell research 9 6 923--32

In vitro culture of human acute myelogenous leukemia (AML) cells in serum-free media: studies of native AML blasts and AML cell lines.

The functional characteristics were compared for acute myelogenous leukemia (AML) cells (native blasts and AML cell lines) cultured in three serum-free media (X-vivo 10,X-vivo 15,[Bio-Whitacker,Walkersville,MD] and StemSpan [Stem Cell Technologies,Vancouver,BC,Canada]) and in medium containing 10% inactivated fetal calf serum (FCS). For native AML blasts the following functions were compared: (1) autonomous and cytokine-dependent proliferation; (2) frequency of clonogenic cell; and (3) constitutive cytokine secretion. AML blast proliferation differed between patients independent of the culture medium used,and clonogenic cells were maintained after in vitro culture in all media. In contrast,constitutive cytokine secretion was higher for cells cultured in StemSpan and FCS-containing medium than for cells cultured in the X-vivo media. Native AML blasts incubated in StemSpan also showed a low frequency of apoptotic cells. The three serum-free media could also be used for long-term expansion of well-characterized AML cell lines,but the optimal medium for cell expansion and cytokine secretion differed between cell lines. We conclude that standardized serum-free culture conditions can be used for in vitro studies of native AML blasts and AML cell lines. View Publication

产品类型：

产品号#：

09600

09650

产品名：

StemSpan™ SFEM

CFU Assay Setup for Custom Hematopoietic Training Courses

9:06

视频

CFU Assay Setup for Custom Hematopoietic Training Courses

发布日期: 05/25/2017

技术手册

Human Colony-Forming Unit (CFU) Assays Using MethoCult™

产品类型：

产品号#：

00217

00217UK

00215

00215GER

00215UK

00602

00603

00606

00607

00608

00609

04436

04064

04100

04230

04236

04431

04434

04444

04464

04531

04535

04545

04536

04564

09300

04035

04330

04034

04044

04435

04445

04534

04544

04437

04447

00215US.4

00215CA.3

00215GER.3

0

产品名：

MethoCult™ SF H4436

MethoCult™ H4034 Optimum 入门试剂盒

MethoCult™ H4100

MethoCult™ H4230

MethoCult™ SF H4236

MethoCult™ H4431

MethoCult™ H4434 Classic

MethoCult™ H4434 Classic 套装

MethoCult™ H4531

MethoCult™ H4535 Enriched，不含EPO

MethoCult™ SF H4536

MethoCult™ H4534 Classic 无 EPO 入门试剂盒

含有10% 牛血清白蛋白（BSA）的 Iscove's MDM

MethoCult™ 不含EPO的H4035 Optimum

MethoCult™ H4330

MethoCult™ H4034 Optimum

MethoCult™ H4435 Enriched

MethoCult™ H4534 Classic（不含 EPO）

MethoCult™ Express

造血祖细胞检测标准化培训课程

科学海报

Complete Serum-Free Culture Kit and Protocols for Culturing High Yields of Functional Mature Neurons from Primary Embryonic Mouse CNS Tissues

产品类型：

Conference：

NEUROSCIENCE 2012

产品号#：

05711

05712

05721

05710

产品名：

NeuroCult™ SM1 神经添加物

Bryja V et al. ( 2006) Nature protocols 1 4 2082--2087

Derivation of mouse embryonic stem cells.

Here we describe a simple and efficient protocol for derivation of germline chimera-competent mouse embryonic stem cells (mESCs) from embryonic day 3.5 (E3.5) blastocysts. The protocol involves the use of early-passage mouse embryonic fibroblast feeders (MEF) and the alternation of fetal bovine serum- and serum replacement (SR)-containing media. As compared to other available protocols for mESCs derivation,our protocol differs in the combination of commercial availability of all reagents,technical simplicity and high efficiency. mESC lines are derived with approximately 50% efficiency (50 independent mESC lines derived from 96 blastocysts). We believe that this protocol could be a good starting point for (i) setting up the derivation of mESC lines in a laboratory and (ii) incorporating further steps to improve efficiency or adapt the protocol to other applications. The whole process (from blastocyst extraction to the freezing of mESC line) usually takes between 15 and 20 d. View Publication

产品类型：

产品号#：

73272

73274

100-1048

产品名：

丝裂霉素C

科学海报

STEMdiff™ Hematopoietic Kit Reproducibly Generates Functional Hematopoietic Progenitor Cells from Human Pluripotent Stem Cells

产品类型：

Conference：

ISSCR 2017

产品号#：

04535

04545

05310

02692

09605

09655

产品名：

MethoCult™ H4535 Enriched，不含EPO

STEMdiff™ 造血试剂盒

StemSpan™红系扩增添加物 (100X)

StemSpan™ SFEM II

搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture

Heat-stable antigen (CD24) as ligand for mouse P-selectin.

A specific in vitro bioassay for measuring erythropoietin levels in human serum and plasma.

In vitro culture of human acute myelogenous leukemia (AML) cells in serum-free media: studies of native AML blasts and AML cell lines.

Derivation of mouse embryonic stem cells.

欢迎您关注STEMCELL Technologies的微信公众平台