搜索结果: 'stemprep kits'

Brochure
EasySep™ Direct Human PBMC Isolation Kit

产品类型：

Cell Isolation Products

品牌：

EasySep，RoboSep

产品号#：

19654

19654RF

产品名：

EasySep™直接人PBMC分离试剂盒

EasySep™直接人PBMC分离试剂盒
Technical Manual
DNA Isolation Using the Genomic DNA Purification Kit

产品类型：

Cell Engineering and Molecular Tools

产品号#：

79020

产品名：

基因组DNA纯化试剂盒
Technical Manual
RNA Isolation Using the Total RNA Purification Kit

产品类型：

Cell Engineering and Molecular Tools

产品号#：

79040

产品名：

总RNA纯化试剂盒
Tech Tip
Frequently Asked Questions on STEMdiff™ Intestinal Organoid Kit
Reference

Yang Y et al. (AUG 2010) Blood 116 7 1114--23

Pediatric mastocytosis-associated KIT extracellular domain mutations exhibit different functional and signaling properties compared with KIT-phosphotransferase domain mutations.

Compared with adults,pediatric mastocytosis has a relatively favorable prognosis. Interestingly,a difference was also observed in the status of c-kit mutations according to the age of onset. Although most adult patients have a D(816)V mutation in phosphotransferase domain (PTD),we have described that half of the children carry mutations in extracellular domain (ECD). KIT-ECD versus KIT-PTD mutants were introduced into rodent Ba/F3,EML,Rat2,and human TF1 cells to investigate their biologic effect. Both ECD and PTD mutations induced constitutive receptor autophosphorylation and ligand-independent proliferation of the 3 hematopoietic cells. Unlike ECD mutants,PTD mutants enhanced cluster formation and up-regulated several mast cell-related antigens in Ba/F3 cells. PTD mutants failed to support colony formation and erythropoietin-mediated erythroid differentiation. ECD and PTD mutants also displayed distinct whole-genome transcriptional profiles in EML cells. We observed differences in their signaling properties: they both activated STAT,whereas AKT was only activated by ECD mutants. Consistently,AKT inhibitor suppressed ECD mutant-dependent proliferation,clonogenicity,and erythroid differentiation. Expression of myristoylated AKT restored erythroid differentiation in EML-PTD cells,suggesting the differential role of AKT in those mutants. Overall,our study implied different pathogenesis of pediatric versus adult mastocytosis,which might explain their diverse phenotypes. View Publication

产品类型：

产品号#：

03231

03434

03444

09500

产品名：

MethoCult™M3231

MethoCult™GF M3434

MethoCult™GF M3434

bit9500血清替代品
Technical Manual
DNA Purification Using the Gel and PCR Clean-up Kit

产品类型：

Cell Engineering and Molecular Tools

产品号#：

79030

产品名：

凝胶和PCR清理试剂盒
Reference

Carter TA et al. ( 2005) Proceedings of the National Academy of Sciences of the United States of America 102 31 11011--11016

Inhibition of drug-resistant mutants of ABL, KIT, and EGF receptor kinases.

To realize the full potential of targeted protein kinase inhibitors for the treatment of cancer,it is important to address the emergence of drug resistance in treated patients. Mutant forms of BCR-ABL,KIT,and the EGF receptor (EGFR) have been found that confer resistance to the drugs imatinib,gefitinib,and erlotinib. The mutations weaken or prevent drug binding,and interestingly,one of the most common sites of mutation in all three kinases is a highly conserved gatekeeper" threonine residue near the kinase active site. We have identified existing clinical compounds that bind and inhibit drug-resistant mutant variants of ABL� View Publication

产品类型：

产品号#：

73082

73084

产品名：

达沙替尼

达沙替尼
STEMCELL Technologies Inc. has just released NEW MesenCult™ Proliferation Kit with MesenPure™ (Mouse), a novel cell culture medium to advance research on mouse mesenchymal stem and progenitor cells (MSCs)

产品类型：

产品号#：

产品名：
New Tools for the Generation of Differentiated CNS Cell Types from Human Pluripotent Stem Cells: STEMdiff™ Neuron, Dopaminergic Neuron and Astrocyte Kits

产品类型：

Conference：

SFN 2015

产品号#：

05832

05835

05839

05850

05857

05870

05875

08520

08530

08540

08550

08560

27845

27945

27840

27865

27940

27965

70901

70902

70905

70909

70913

08581

08582

产品名：

STEMdiff™ 神经玫瑰花环结构选择试剂

STEMdiff™神经诱导培养基

STEMdiff™神经诱导培养基

STEMdiff™SMADi神经诱导试剂盒

STEMdiff™SMADi神经诱导试剂盒
Technical Manual
Generation of Human Intestinal Organoids Using STEMdiff™ Intestinal Organoid Kit

产品类型：

Cell Culture Media and Supplements

产品号#：

05140

05145

产品名：

STEMdiff™肠道类器官试剂盒

STEMdiff™肠道类器官生长培养基
2:22

Video
How ALDEFLUOR™ ALDH Assay Kit Detects Normal and Cancer Progenitor Cells

发布日期: 12/4/17
Technical Bulletin
In Vitro Drug Screening with the StemSpan™ Leukemic Cell Culture Kit

产品类型：

Cell Culture Media and Supplements

细胞类型：

癌细胞及细胞系，白血病/淋巴瘤细胞，造血干/祖细胞

产品号#：

09605

02691

72332

09720

产品名：

StemSpan™SFEM II

StemSpan™CD34+扩增补充（10X）

UM729

StemSpan™白血病细胞培养试剂盒