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AggreWell™HT

高通量96孔微孔培养板,用于简便且可重复的生成类器官 胚状体和类球体

产品号 #(选择产品)

产品号 #200-0563_C

高通量96孔微孔培养板,用于简便且可重复的生成类器官 胚状体和类球体

产品优势

  • 900 μm微孔简化了高通量工作流程,每微孔可形成32个聚集体,非常适合扩大规模
  • 确保重复性,细胞聚集体大小一致且均匀
  • 通过减少弯月面特性,提升成像效果,获得更清晰的明场图像
  • 容易形成聚集体,每微孔至多50个
  • 无缝过渡与跨AggreWell™格式的兼容性
在不同 AggreWell™ 版式之间实现无缝过渡,兼容性强

产品组分包括

  • AggreWell™HT 96孔板
    • 1块(产品号#200-0563)
    • 5块(产品号#200-0570)

总览

使用AggreWell™HT简化3D培养工作流程,这是一种高通量96孔微孔板,旨在优化均匀细胞聚集体的形成。无论生成的是类球体还是胚状体(EBs),AggreWell™HT每微孔最多可生成32个聚集体——多于传统的超低附着力培养板——从而获得更高的统计效能、更低的变异性以及更可靠的实验结果。

AggreWell™HT非常适合稀有细胞类型或有限样本的实验,每微孔仅需50个细胞,确保您可以最大限度地利用样本。该培养板兼容自动化液体处理系统,同时配备 减少弯月面设计,可提升明场成像的质量与便捷性,进一步提高工作效率。注:抗粘附冲洗液(产品号# 07010)是最佳的EB和类球体形成所必需的。

AggreWell™HT兼容在AggreWell™400AggreWell™800板式中的开发应用,方便高效地扩展实验流程。了解更多关于如何使用AggreWell™联系我们了解更多信息。

亚型
培养皿与培养板
 
应用
细胞培养,鉴定,分化,类器官培养,球状体培养,细胞毒性检测
 
品牌
AggreWell
 
研究领域
疾病建模,药物发现和毒理检测,神经科学,类器官
 

实验数据

AggreWell™HT features a meniscus-reducing feature and allows for the formation of aggregates from various initial seeding densities.

Figure 1. AggreWell™HT Promotes Cell Aggregation Across 32 Individual Microwells

(A) AggreWell™HT single-well cross section displaying the microwell array, featuring 32 microwells, and the meniscus-reducing step feature. Visualization of human embryonic stem cell (hESC) aggregates formed in AggreWell™HT after 72 hours from an initial seeding density of (B) 1,000 cells per microwell and (C) 10,000 cells per microwell.

AggreWell™HT promotes neural induction of embryonic stem cells to the same extent as AggreWell™800.

Figure 2. AggreWell™HT Promotes Neural Induction in Embryoid Bodies Comparable to AggreWell™800

Human embryonic stem (ES) cell (H9) aggregates cultured in (A) AggreWell™HT and (B) AggreWell™800 microwell plates 24 hours post-seeding; scale bar = 500 μm. Morphology of Day 6 neural progenitor cell rosettes differentiated using an embryoid body (EB)-based protocol with Neural Induction Medium (Catalog #05835) in (C) AggreWell™HT and (D) AggreWell™800 microwell plates; scale bar = 500 μm. (E) Neural marker expression of PAX6 and SOX1 is equivalent in both AggreWell™ plates at Day 18 following neural induction of H9 ES cells in both AggreWell™HT and AggreWell™800 plates, as determined by flow cytometry (n = 4). Error bars = SD.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
200-0563
Lot #
All
Language
English
Catalog #
200-0570
Lot #
All
Language
English
质量保证:

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