若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

抗小鼠CD11c抗体,克隆N418

抗小鼠CD11c的仓鼠(亚美尼亚)单克隆IgG抗体

产品号 #(选择产品)

产品号 #60002_C

抗小鼠CD11c的仓鼠(亚美尼亚)单克隆IgG抗体

总览

N418抗体可靶向CD11c(αX整合素)。CD11c是一种150 kDa的1型跨膜糖蛋白,其通过与CD18(β2整合素)非共价结合形成异二聚体细胞表面黏附受体。CD11c/CD18受体通过结合iC3b、纤维蛋白原和CD54等配体,参与多种免疫应答过程,包括细胞迁移、刺激单核细胞和巨噬细胞产生细胞因子、T细胞扩增、白细胞募集和吞噬作用。在小鼠中,CD11c在树突状细胞、巨噬细胞、单核细胞、粒细胞、NK细胞和部分T细胞上表达。
N418抗体克隆已通过验证,适用于EasySep™试剂盒(包括EasySep™小鼠CD11c正选试剂盒II,产品号 #18780)分离的细胞纯度检测。

亚型
一抗
 
靶抗原
CD11c
 
别名
alphaX整合素,CR4,整合素alphaX链,p150
 
活性物种
小鼠
 
偶联
Biotin 或 生物素,FITC,PE,未偶联的
 
宿主物种
Hamster
 
细胞类型
树突状细胞(DCs)
 
种属
小鼠
 
应用
CyTOF,流式细胞术,免疫细胞化学,免疫荧光,免疫组化,免疫沉淀
 
研究领域
免疫
 
克隆
N418
 
基因编号
16411
 
同种型
IgG
 

实验数据

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with EasySep™ Mouse CD11c Positive Selection Kit II and labeled with Anti-Mouse CD11c Antibody, Clone N418, Alexa Fluor® 488. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of the start cells with an Armenian hamster IgG Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (solid line histogram). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with EasySep™ Mouse CD11c Positive Selection Kit II and labeled with Anti-Mouse CD11c Antibody, Clone N418, Alexa Fluor® 488 and an anti-mouse CD317 antibody, APC. (C) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, Alexa Fluor® 488 and an anti-mouse MHC class II antibody, APC. (D) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with an Armenian hamster IgG Alexa Fluor® 488 isotype control antibody and an anti-mouse MHC class II antibody, APC.

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with EasySep™ Mouse CD11c Positive Selection Kit II and labeled with Anti-Mouse CD11c Antibody, Clone N418, PE. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of the start cells with an Armenian hamster IgG PE isotype control antibody is shown in the bottom panel (solid line histogram). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with EasySep™ Mouse CD11c Positive Selection Kit II and labeled with Anti-Mouse CD11c Antibody, Clone N418, PE and an anti-mouse CD317 antibody, APC. (C) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, PE and an anti-mouse MHC class II antibody, APC. (D) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with an Armenian hamster IgG PE isotype control antibody and an anti-mouse MHC class II antibody, APC.

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, followed by anti-hamster (Armenian) IgG, FITC and anti-mouse MHC class II, APC. (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a hamster (Armenian) IgG, isotype control antibody followed by anti-hamster (Armenian) IgG, FITC and anti-mouse MHC class II, APC.

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488 (Catalog #60030AD). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with an Armenian hamster IgG APC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488. (C) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, APC and an anti-mouse MHC class II antibody, FITC. (D) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with an Armenian hamster IgG APC isotype control antibody and an anti-mouse MHC class II antibody, FITC.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a biotinylated Armenian hamster IgG, isotype control antibody followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC. (C) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, Biotin, followed by SAV APC and an anti-mouse MHC class II antibody, FITC. (D) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a biotinylated Armenian hamster IgG, isotype control antibody followed by SAV APC and an anti-mouse MHC class II antibody, FITC.

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with an Armenian hamster IgG FITC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, PerCP-Cy5.5 and an anti-mouse CD317 antibody, APC. (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with an Armenian hamster IgG PerCP-Cy5.5 isotype control antibody and an anti-mouse CD317 antibody, APC. (C) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, PerCP-Cy5.5 and an anti-mouse MHC class II antibody, FITC. (D) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with an Armenian hamster IgG PerCP-Cy5.5 isotype control antibody and an anti-mouse MHC class II antibody, FITC.

Figure 8. Data for PB-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with an Armenian hamster IgG Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60002AD
Lot #
All
Language
English
Catalog #
60002AZ, 60002AZ.1
Lot #
All
Language
English
Catalog #
100-0442, 100-0441, 60002BT
Lot #
All
Language
English
Catalog #
100-0443, 60002FI
Lot #
All
Language
English
Catalog #
60002PB, 60002PB.1
Lot #
All
Language
English
Catalog #
100-0446, 100-0445
Lot #
All
Language
English
Catalog #
60002PS
Lot #
All
Language
English
Catalog #
100-0440
Lot #
All
Language
English
Catalog #
60002
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60002AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60002AZ, 60002AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0442, 100-0441, 60002BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0443, 60002FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60002PB, 60002PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0446, 100-0445
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60002PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0440
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60002
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (3)

文献 (1)

Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering. Haraguchi Y et al. Journal of Tissue Engineering and Regenerative Medicine 2015 DEC

Abstract

In this study, a simple three-dimensional (3D) suspension culture method for the expansion and cardiac differentiation of human induced pluripotent stem cells (hiPSCs) is reported. The culture methods were easily adapted from two-dimensional (2D) to 3D culture without any additional manipulations. When hiPSCs were directly applied to 3D culture from 2D in a single-cell suspension, only a few aggregated cells were observed. However, after 3 days, culture of the small hiPSC aggregates in a spinner flask at the optimal agitation rate created aggregates which were capable of cell passages from the single-cell suspension. Cell numbers increased to approximately 10-fold after 12 days of culture. The undifferentiated state of expanded hiPSCs was confirmed by flow cytometry, immunocytochemistry and quantitative RT-PCR, and the hiPSCs differentiated into three germ layers. When the hiPSCs were subsequently cultured in a flask using cardiac differentiation medium, expression of cardiac cell-specific genes and beating cardiomyocytes were observed. Furthermore, the culture of hiPSCs on Matrigel-coated dishes with serum-free medium containing activin A, BMP4 and FGF-2 enabled it to generate robust spontaneous beating cardiomyocytes and these cells expressed several cardiac cell-related genes, including HCN4, MLC-2a and MLC-2v. This suggests that the expanded hiPSCs might maintain the potential to differentiate into several types of cardiomyocytes, including pacemakers. Moreover, when cardiac cell sheets were fabricated using differentiated cardiomyocytes, they beat spontaneously and synchronously, indicating electrically communicative tissue. This simple culture system might enable the generation of sufficient amounts of beating cardiomyocytes for use in cardiac regenerative medicine and tissue engineering.

更多信息

更多信息
种属 Mouse
克隆 N418
Gene Id 16411
Alternative Names alphaX integrin, CR4, integrin alphaX chain, p150
同种型/isotype IgG
法律声明:

Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. 质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息,请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.