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CD34+祖细胞重编程试剂盒

包含从外周血中分离和扩增CD34+祖细胞及将其重编程为iPS细胞的工作流程

产品号 #(选择产品)

产品号 #05925_C

包含从外周血中分离和扩增CD34+祖细胞及将其重编程为iPS细胞的工作流程

产品优势

  • 优化了从外周血扩增的CD34+祖细胞的富集、扩增和重编程;
  • 与传统的人胚胎干细胞(hES)培养基相比,iPS细胞重编程效率更高,iPS细胞克隆形成频率更高;
  • 可快速形成具有高质量iPS细胞样形态的,有利于鉴定和亚克隆;
  • 与TeSR™和STEMdiff™产品无缝衔接,用于iPS细胞系的下游维持和分化。

产品组分包括

  • SepMate™-50(产品号:#15440);
  • Lymphoprep™(产品号:#18060);
  • 人全血CD34+细胞分选试剂盒(产品号:#15086);
  • RosetteSep™人造血祖细胞富集抗体混合物,3 × 2ml;
  • EasySep™Human CD34 正选抗体混合物, 0.4 mL;
  • EasySep™Dextran RapidSpheres™50100磁珠,1 mL;
  • StemSpan™SFEM II(产品号:#09605);
  • StemSpan™CD34+扩增添加物(产品号:#02691);
  • ReproTeSR™(产品号:#05926);
  • TeSR™-E7™/ReproTeSR™基础培养基;
  • ReproTeSR™25X添加物
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总览

CD34+祖细胞重编程试剂盒包含一套完整的工具和试剂,用于从人外周血样本中富集、扩增和重编程CD34+祖细胞。

亚型
专用培养基
 
细胞类型
造血干/祖细胞,多能干细胞
 
种属

 
应用
细胞培养,重编程
 
品牌
StemSpan,TeSR
 
研究领域
干细胞生物学
 
制剂类别
无血清
 

实验数据

Typical Performance of the EasySep™ Complete Kit for Human Whole Blood CD34+ Cells

Figure 1. Typical Performance of the EasySep™ Complete Kit for Human Whole Blood CD34+ Cells

(A) In whole PB, the proportion of CD34+/CD45+ cells is typically very low (<1%). (B) In the RosetteSep™ enrichment step, unwanted cells are removed during density gradient centrifugation. This step enriches the population of CD34+/CD45+ cells (19.8%) in this example). (C) After positive selection of CD34+ cells using EasySep™, a highly pure population of CD34+/CD45+ cells (96.2% in this example) can be obtained. Values are reported as a percentage of viable CD45+ cells.

StemSpan™ SFEM II and CD34+ Expansion Supplement Support Expansion of CD34+ Cells

Figure 2. StemSpan™ SFEM II and CD34+ Expansion Supplement Support Expansion of CD34+ Cells

TNC: Total Nucleated Cell

Schematic of ReproTeSR™ Reprogramming Timeline

Figure 3. Schematic of ReproTeSR™ Reprogramming Timeline

ReproTeSR™ is used during the entire induction phase of reprogramming (day 3 to 21). On days 3 and 5, ReproTeSR™ is added to StemSpan™ growth media (in a fed-batch manner) to facilitate attachment of transfected cells. Attached cells are further cultured in ReproTeSR™ with daily full media changes until putative iPS cell colonies emerge (days 21-28). iPS cell colonies can then be isolated and propagated in TeSR™ media. (mTeSR™1, TeSR™2, TeSR™-E8™).

Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Figure 4. Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Efficiency of reprogramming (A) erythroid cells, or (B) CD34+ cells using episomal reprogramming vectors is higher in ReproTeSR™ medium compared to in KOSR-containing hESC medium. Data shown are mean +/- SEM, erythroid cells n=4, CD34+ cells n=5.

ReproTeSR™ Generates iPS Cell Colonies With Superior Colony Morphology

Figure 5. ReproTeSR™ Generates iPS Cell Colonies With Superior Colony Morphology

Representative images of iPS cell colonies generated from isolated CD34+ progenitor cells using (A) ReproTeSR™ and (B) hESC) medium. iPS cell colonies produced using ReproTeSR™ exhibit more defined borders, compact morphology and reduced differentiation compared with hESC medium. 200X magnification.

Reprogramming Efficiency of CD34+ and Erythroid Progenitor Cells With ReproTeSR™

Figure 6. Reprogramming Efficiency of CD34+ and Erythroid Progenitor Cells With ReproTeSR™

Average values in bold (range).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Technical Manual
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Catalog #
05925
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

更多信息

更多信息
种属 Human
配方类别 Serum-Free
质量保证:

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