EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #03804_C
半固体甲基纤维素基杂交瘤筛选和克隆培养基，含HAT（含血清）
若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
总览
使用此基于甲基纤维素的培养基，可一步完成杂交瘤细胞筛选和克隆，从而节省研究时间和资源。ClonaCell™-HY 培养基 D 可帮助您轻松分离具有单克隆可能性高的单个克隆，以便进一步筛选和扩增。
杂交瘤克隆通常具有广泛的生长速率和生产力；使用 ClonaCell™-HY 培养基 D 克隆时，细胞会形成可见且离散的单克隆集落，避免因过度生长而丧失稀有的高产克隆。杂交瘤集落可以通过手动或机器人方法从半固体培养基中轻松挑取，并转移至液体培养基中进行筛选和扩增。
ClonaCell™-HY 培养基 D 是一种含血清的半固体培养基，内含筛选剂次黄嘌呤、氨基蝶呤和胸苷（HAT）。该培养基已验证可用于小鼠和大鼠杂交瘤的开发，并已报道兼容用于从多种宿主动物（包括人、小鼠、大鼠及仓鼠）来源的淋巴细胞制备和克隆杂交瘤细胞（例如 Wilson JR et al. Antiviral Res, 2016）。
为何选择半固体克隆？
•形成物理分离的独立克隆，便于挑取。
•相较于液体培养，使用半固体培养基进行同时筛选和克隆，能更轻松地单独筛选稀有和高产克隆。
•与有限稀释法筛选和克隆相比，能够在更短时间内用更少资源获得单克隆细胞系。
更多高效杂交瘤克隆和生成相关的产品，请访问我们的 ClonaCell™ 产品页面。
包含
• DMEM培养基
• 甲基纤维素
• 血清
• 次黄嘌呤、氨基蝶呤和胸苷 (HAT)
• 庆大霉素
• 2-巯基乙醇
• 酚红
• L-谷氨酰胺及其他添加物
• 其他成分
分类
半固体培养基，专用培养基
细胞类型
杂交瘤细胞
种属
人，小鼠，其它细胞系，大鼠
应用
细胞培养，半固体克隆
品牌
ClonaCell
研究领域
抗体制备，癌症，细胞系制备，药物发现和毒理检测，杂交瘤制备
制剂类别
甲基纤维素基
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (9)
常见问题
文献 (21)
An influenza A virus (H7N9) anti-neuraminidase monoclonal antibody with prophylactic and therapeutic activity in vivo Antiviral Research 2016 NOV
Abstract
Zoonotic A(H7N9) avian influenza viruses emerged in China in 2013 and continue to be a threat to human public health, having infected over 800 individuals with a mortality rate approaching 40%. Treatment options for people infected with A(H7N9) include the use of neuraminidase (NA) inhibitors. However, like other influenza viruses, A(H7N9) can become resistant to these drugs. The use of monoclonal antibodies is a rapidly developing strategy for controlling influenza virus infection. Here we generated a murine monoclonal antibody (3c10-3) directed against the NA of A(H7N9) and show that prophylactic systemic administration of 3c10-3 fully protected mice from lethal challenge with wild-type A/Anhui/1/2013 (H7N9). Further, post-infection treatment with a single systemic dose of 3c10-3 at either 24, 48 or 72 h post A(H7N9) challenge resulted in both dose- and time-dependent protection of up to 100% of mice, demonstrating therapeutic potential for 3c10-3. Epitope mapping revealed that 3c10-3 binds near the enzyme active site of NA, and functional characterization showed that 3c10-3 inhibits the enzyme activity of NA and restricts the cell-to-cell spread of the virus in cultured cells. Affinity analysis also revealed that 3c10-3 binds equally well to recombinant NA of wild-type A/Anhui/1/2013 and to a variant NA carrying a R289K mutation known to infer NAI resistance. These results suggest that 3c10-3 has the potential to be used as a therapeutic to treat A(H7N9) infections either as an alternative to, or in combination with, current NA antiviral inhibitors.
Immunodominant West Nile virus T cell epitopes are fewer in number and fashionably late The Journal of Immunology 2016 MAY
Abstract
Class I HLA molecules mark infected cells for immune targeting by presenting pathogen-encoded peptides on the cell surface. Characterization of viral peptides unique to infected cells is important for understanding CD8(+) T cell responses and for the development of T cell-based immunotherapies. Having previously reported a series of West Nile virus (WNV) epitopes that are naturally presented by HLA-A*02:01, in this study we generated TCR mimic (TCRm) mAbs to three of these peptide/HLA complexes-the immunodominant SVG9 (E protein), the subdominant SLF9 (NS4B protein), and the immunorecessive YTM9 (NS3 protein)-and used these TCRm mAbs to stain WNV-infected cell lines and primary APCs. TCRm staining of WNV-infected cells demonstrated that the immunorecessive YTM9 appeared several hours earlier and at 5- to 10-fold greater density than the more immunogenic SLF9 and SVG9 ligands, respectively. Moreover, staining following inhibition of the TAP demonstrated that all three viral ligands were presented in a TAP-dependent manner despite originating from different cellular compartments. To our knowledge, this study represents the first use of TCRm mAbs to define the kinetics and magnitude of HLA presentation for a series of epitopes encoded by one virus, and the results depict a pattern whereby individual epitopes differ considerably in abundance and availability. The observations that immunodominant ligands can be found at lower levels and at later time points after infection suggest that a reevaluation of the factors that combine to shape T cell reactivity may be warranted.
Deletion of a Yci1 Domain Protein of Candida albicans Allows Homothallic Mating in MTL Heterozygous Cells mBio 2016 MAY
Abstract
It has been proposed that the ancestral fungus was mating competent and homothallic. However, many mating-competent fungi were initially classified as asexual because their mating capacity was hidden behind layers of regulation. For efficient in vitro mating, the essentially obligate diploid ascomycete pathogen Candida albicans has to change its mating type locus from heterozygous MTL a /α to homozygous MTL a / a or MTL α/α and then undergo an environmentally controlled epigenetic switch to the mating-competent opaque form. These requirements greatly reduce the potential for C. albicans mating. Deletion of the Yci1 domain gene OFR1 bypasses the need for C. albicans cells to change the mating type locus from heterozygous to homozygous prior to switching to the opaque form and mating and allows homothallic mating of MTL heterozygous strains. This bypass is carbon source dependent and does not occur when cells are grown on glucose. Transcriptional profiling of ofr1 mutant cells shows that in addition to regulating cell type and mating circuitry, Ofr1 is needed for proper regulation of histone and chitin biosynthesis gene expression. It appears that OFR1 is a key regulator in C. albicans and functions in part to maintain the cryptic mating phenotype of the pathogen.
更多信息
| 种属 | Human, Mouse, Other, Rat |
|---|---|
| Contains | • DMEM • Methylcellulose • Serum • Hypoxanthine, aminopterin, and thymidine (HAT) • Gentamicin • 2-Mercaptoethanol • Phenol red • L-Glutamine and other supplements • Other ingredients |
| 配方类别 | Methylcellulose-Based |
相关产品
-
ClonaCell™-HY杂交瘤试剂盒完整的杂交瘤生成试剂盒
-
ClonaCell™-HY Medium次黄嘌呤胸腺嘧啶杂交瘤生长培养基（含血清）
-
ClonaCell™-HY Medium D无HAT半固体甲基纤维素基杂交瘤筛选和克隆培养基，不含HAT（含血清）
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE. Safety Statement: CA WARNING: This product can expose you to Aminopterin which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE. Safety Statement: CA WARNING: This product can expose you to Aminopterin which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov
