Easy 250 EasySep™磁铁

用于大规模免疫磁性分离的磁铁

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产品号 #（选择产品）

产品号 #100-0821_C

无柱式免疫磁性分离磁铁

产品优势

高效。单次分离中即可从大容量样品中分离细胞，提高细胞分离通量。
快速。只需 20 分钟即可从最多 225 mL 的样本中分离免疫细胞。
高纯度。分离后高达99%纯度的有功能的活细胞。
灵活。兼容多种类型的EasySep™细胞分离试剂盒以获得您感兴趣的特定细胞类型。

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总览

实验数据

产品说明书及文档

应用领域

总览

"轻松高效地从大体积样本（例如白细胞单采术样本和全血）中提高手动细胞分离通量。无需拆分细胞悬浮液并进行多轮分离，使用 Easy 250 EasySep™ 磁铁，您一次分离即可处理多达 225 mL 的细胞悬液和 1.25 x 10^10个细胞。

将Easy 250 EasySep™磁铁与标准T-75厘米²细胞培养瓶和EasySep™试剂配合使用，即可从大容量样品中分离细胞，而无需使用分选柱。磁铁内部强大的高梯度磁场可分离带有磁性 EasySep™ 颗粒标记的细胞，您只需移取未标记的细胞组分，即可获得可立即用于下游应用的细胞。EasySep™无柱技术可节省时间，并获得高纯度细胞。

不确定使用哪种磁铁？访问我们的EasySep™细胞分离磁铁页面比较不同的选择，并为您的研究选择合适的磁铁。

了解更多关于免疫磁性分选的知识EasySep™技术产品。"

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人

应用
细胞分选

品牌
EasySep

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实验数据

Figure 1. Using the Easy 250 EasySep™ Magnet Results in the Isolation of Highly Purified Cells of Interest

Human immune cells were isolated from processed leukopaks using the corresponding (A) EasySep™ negative selection kits or (B) EasySep™ positive selection kits (see Table 1). Cell purity was measured before isolation (Start) and after isolation with the Easy 250 EasySep™ Magnet (Easy 250), EasySep™ Magnet (Purple) or “The Big Easy” EasySep™ Magnet (Silver). Purity was assessed by staining with cell surface markers for pan-T cells (CD3+), CD4+ T cells (CD3+CD4+), CD8+ T cells (CD3+CD8+), or monocytes (CD14+CD45+), and analyzed by flow cytometry. Data shown as mean ± SD; n = 4 - 12.

Figure 2. Easy 250 EasySep™ Magnet Can Be Used to Isolate Mononuclear Cells from Whole Blood and Leukopak Samples of Up to 125 mL

Mononuclear cells (MNCs) were isolated using the EasySep™ Direct Human PBMC Isolation Kit (Catalog #19654) from large whole blood samples (25 - 125 mL) or unprocessed leukopaks (25 - 125 mL) with the Easy 250 EasySep™ Magnet (Easy 250) and from small samples (1 mL whole blood) with the EasySep™ Magnet (Purple). (A) Representative flow cytometry plots show the MNC fraction (Glycophorin A- CD42b-). In the above example, the mononuclear cell content of the whole blood start sample (lysed by ammonium chloride) and non-lysed final isolated fraction is 28.9% and 97.1% (not gated on CD45), respectively. (B) Cell purity was measured before (Start) and after isolation (Easy 250, Purple) based on viable cells. Cells were counted and analyzed by flow cytometry. Data shown as mean ± SD; n = 7 - 9.