细胞外囊泡SEC柱

用于从生物液体中分离细胞外囊泡的分子排阻色谱柱

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产品号 #（选择产品）

产品号 #100-0414_C

用于从生物液体中分离细胞外囊泡的分子排阻色谱柱

产品优势

快速，易于使用的方案
可重复使用（最多五次）

产品组分包括

0.5 mL细胞外囊泡SEC柱

10个单位（产品号#100-0414）

2 mL细胞外囊泡SEC柱

5个单位（产品号#100-0415）

20 mL细胞外囊泡SEC柱

3个单位（产品号#100-0416）

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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

D-PBS (Without Ca++ and Mg++)
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Costar® Microcentrifuge Tubes
Non-sterile, clear, polypropylene snap-cap microcentrifuge tubes
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

细胞外囊泡分子排阻色谱（SEC）柱可以从多种生物基质中分离和纯化细胞外囊泡（EV），包括血浆、血清和细胞培养基。与其他EV分离方法相比，该方法能有效将EV从循环蛋白中分离，且对囊泡的改变（包括功能或大小）最小。SEC柱分离快速简便，分离出的EV适用于下游分析，如流式细胞术、蛋白质印迹、核酸提取和/或功能分析。SEC柱有0.5 mL（10柱/包），2 mL（5柱/包）和20 mL（3柱/包）大小，以支持不同的样品体积，可使用多达五次。

亚型
分子筛色谱柱

种属
人，小鼠，非人灵长类，其它细胞系，大鼠

应用
细胞外囊泡分选

研究领域
细胞外囊泡研究

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实验数据

$Graphs showing the fractions in which particles and proteins, isolated from human plasma using the 0.5 mL Extracellular Vesicle SEC Column, were detected by nanoparticle tracking analysis and bicinchoninic acid assays, respectively.$

Figure 1. Isolation of Extracellular Vesicles from Plasma Using a 0.5 mL Extracellular Vesicle SEC Column

The 0.5 mL Extracellular Vesicle SEC Column was loaded with 0.5 mL of human plasma. 100 μL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis (NTA) and bicinchoninic acid (BCA) assays, respectively. EVs were detected in fractions 9 - 14, while proteins were detected in fractions 15 onwards.

$Graphs showing the fractions in which particles and proteins, isolated from human plasma using the 2 mL Extracellular Vesicle SEC Column, were detected by nanoparticle tracking analysis and bicinchoninic acid assays, respectively.$

Figure 2. Isolation of Extracellular Vesicles from Plasma Using a 2 mL Extracellular Vesicle SEC Column

The 2 mL Extracellular Vesicle SEC Column was loaded with 2 mL of human plasma. 500 μL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis and bicinchoninic acid assays, respectively. EVs were detected in fractions 7 - 10, while proteins were detected in fractions 11 onwards.

$Graphs showing the fractions in which particles and proteins, isolated from concentrated serum-free medium conditioned by mesenchymal stromal cell culture, using the 20 mL Extracellular Vesicle SEC Column, were detected by nanoparticle tracking analysis and bicinchoninic acid assays, respectively.$

Figure 3. Isolation of Extracellular Vesicles from Conditioned Medium Using a 20 mL Extracellular Vesicle SEC Column

The 20 mL Extracellular Vesicle SEC Column was loaded with 20 mL of 10-fold concentrated serum-free medium conditioned by mesenchymal stromal cell (MSC) culture. 1 mL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis and bicinchoninic acid assays, respectively. EVs were detected in fractions 15 - 30, while proteins were detected in fractions 35 onwards. Fractions 31-33, which are outside of the typical EV elution range, may still contain EVs with low protein contamination for serum-free media samples.