EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #07806_C
用于新鲜血液样本中去除红细胞并分离有核细胞
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总览
HetaSep™ 是一种红细胞聚集剂，用于快速分离全血中的有核细胞和红细胞（RBC）。聚集的红细胞比分散的细胞沉降得更快。通过控制沉淀时间和/或离心速度，可在上清液中回收大部分的有核细胞。通过小心地收集有核细胞，红细胞的去除率大约可达95-99%。HetaSep™ 含有6% w/v 羟乙基淀粉。
磁体兼容性
亚型
细胞分选试剂盒
细胞类型
粒细胞及其亚群，白细胞，红细胞
种属
人
样本来源
Cord Blood，Whole Blood
筛选方法
Negative
应用
细胞分选
品牌
HetaSep
研究领域
免疫，干细胞生物学
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
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相关材料与文献
技术资料 (7)
文献 (2)
Response of Neutrophils to Extracellular Haemoglobin and LTA in Human Blood System. EBioMedicine 2015 MAR
Abstract
BACKGROUND Haemolytic infection lyses red blood cells, releasing haemoglobin (Hb) into the plasma. Although recent studies showed that immune cells recognize redox-active cytotoxic extracellular Hb (metHb) bound to pathogen-associated molecular patterns (PAMPs), currently available information is limited to experiments performed in defined conditions using single cell lines. Therefore, a systemic approach targeting primary whole blood cells is required to better understand the cellular immune defence against metHb and PAMPs, when under a haemolytic infection. METHODS We investigated how human white blood cells, including neutrophils, respond to metHb and lipoteichoic acid (LTA) by measuring reactive oxygen species (ROS), signalling mediators (ERK and p38), NF-κB, cytokines, elastase secretion and cell activation markers. FINDINGS metHb activates NF-κB in TLR2-expressing HEK293 cells but not in normal or TLR9-expressing HEK293 cells. Treatment of isolated neutrophils with metHb increased production of ROS and expressions of IL-8, TNFα, and CD11b, which were further enhanced by metHb + LTA complex. While LTA stimulated the survival of neutrophils, it caused apoptotic cell death when complexed with metHb. The activation of neutrophils by metHb + LTA was subdued by the presence of other types of white blood cells. INTERPRETATION metHb and metHb + LTA complex are ligands of TLR2, inducing an unconventional TLR signalling pathway. Neutrophils are a highly sensitive cell type to metHb + LTA complex. During a haemolytic infection, white blood cells in the vicinity crosstalk to modulate neutrophil TLR-signalling induced by metHb and LTA.
Mucosal innate and adaptive immune responses against herpes simplex virus type 2 in a humanized mouse model. Journal of virology 2009 OCT
Abstract
Genital herpes, caused by herpes simplex virus type 2 (HSV-2), is one of the most prevalent sexually transmitted diseases worldwide and a risk factor for acquiring human immunodeficiency virus. Although many vaccine candidates have shown promising results in animal models, they have failed to be effective in human trials. In this study, a humanized mouse strain was evaluated as a potential preclinical model for studying human immune responses to HSV-2 infection and vaccination. Immunodeficient mouse strains were examined for their abilities to develop human innate and adaptive immune cells after transplantation of human umbilical cord stem cells. A RAG2(-/-) gammac(-/-) mouse strain with a BALB/c background was chosen as the most appropriate model and was then examined for its ability to mount innate and adaptive immune responses to intravaginal HSV-2 infection and immunization. After primary infection, human cells in the lymph nodes were able to generate a protective innate immune response and produce gamma interferon (IFN-gamma). After intravaginal immunization and infection, human T cells and NK cells were found in the genital tract and iliac lymph nodes. In addition, human T cells in the spleen, lymph nodes, and vaginal tract were able to respond to stimulation with HSV-2 antigens by replicating and producing IFN-gamma. Human B cells were also able to produce HSV-2-specific immunoglobulin G. These adaptive responses were also shown to be protective and reduce local viral replication in the genital tract. This approach provides a means for studying human immune responses in vivo using a small-animal model and may become an important preclinical tool.
更多信息
| 种属 | Human |
|---|---|
| Magnet Compatibility | |
| 样本来源 | Cord Blood, Whole Blood |
| Selection Method | Negative |
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