EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #06010_C
用于建立和维持人肠道类器官的培养基
若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
Interested in trying STEMCELL’s organoid products for your intestinal research? Fill out the form to request information about introductory offers.
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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
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Gentle Cell Dissociation ReagentcGMP, enzyme-free cell dissociation reagent
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Organoid Culture PlatesCell culture plates for easy and reproducible generation of organoids
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DMEM/F-12 with 15 mM HEPESDulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
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Y-27632 (Dihydrochloride)RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
What Our Scientist Says
Organoids have truly expanded the limits of what's possible for in vitro studies of the intestinal epithelium. By providing optimized culture media and robust, approachable protocols, we are making these technologies more accessible to researchers.
Ryan ConderAssociate Director, Gastrointestinal Biology
总览
使用该完整培养基配方和优化的实验方案，构建和维持肠道类器官，以模拟成年肠上皮的关键特性。凭借该易于操作且有效的实验流程，可在一周内自人体肠隐窝衍生出类器官；富集的干细胞群促进了不同供体来源的类器官的生长，即使是原本较难培养的供体样本也能获得良好结果。
在IntestiCult™ 类器官生长培养基 (人)中生长的类器官包含一个由具有极性的肠上皮细胞层包围而成的功能性腔体结构。为满足多样化的建模应用，这些类器官可在三维结构中，或在浸没式单层培养或气液界面（ALI）培养的二维结构中，通过 IntestiCult™ 类器官分化培养基（人）（产品号 #100-0214）实现进一步分化。
肠类器官培养的应用包括研究肠道上皮的发育和功能、构建肠道疾病模型，以及在肠道模型中筛选分子的有效性和毒性。肠类器官培养还可用于研究成体干细胞特性及再生治疗方法。
欢迎通过我们的免费点播课程学习人肠类器官的培养方法，或浏览关于使用 IntestiCult™ 进行类器官培养流程的常见问题（FAQs）。此外，您还可以下载我们的电子书《Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™》，获取肠类器官培养方案的精选合集。
如果您打算将本产品用于商业目的，请通过www.huborganoids.nl与HUB Organoids B.V.联系，以获取商业用途许可或HUB Organoids B.V.许可的相关说明。
在IntestiCult™ 类器官生长培养基 (人)中生长的类器官包含一个由具有极性的肠上皮细胞层包围而成的功能性腔体结构。为满足多样化的建模应用，这些类器官可在三维结构中，或在浸没式单层培养或气液界面（ALI）培养的二维结构中，通过 IntestiCult™ 类器官分化培养基（人）（产品号 #100-0214）实现进一步分化。
肠类器官培养的应用包括研究肠道上皮的发育和功能、构建肠道疾病模型，以及在肠道模型中筛选分子的有效性和毒性。肠类器官培养还可用于研究成体干细胞特性及再生治疗方法。
欢迎通过我们的免费点播课程学习人肠类器官的培养方法，或浏览关于使用 IntestiCult™ 进行类器官培养流程的常见问题（FAQs）。此外，您还可以下载我们的电子书《Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™》，获取肠类器官培养方案的精选合集。
如果您打算将本产品用于商业目的，请通过www.huborganoids.nl与HUB Organoids B.V.联系，以获取商业用途许可或HUB Organoids B.V.许可的相关说明。
在IntestiCult™ 类器官生长培养基 (人)中生长的类器官包含一个由具有极性的肠上皮细胞层包围而成的功能性腔体结构。为满足多样化的建模应用，这些类器官可在三维结构中，或在浸没式单层培养或气液界面（ALI）培养的二维结构中，通过 IntestiCult™ 类器官分化培养基（人）（产品号 #100-0214）实现进一步分化。
肠类器官培养的应用包括研究肠道上皮的发育和功能、构建肠道疾病模型，以及在肠道模型中筛选分子的有效性和毒性。肠类器官培养还可用于研究成体干细胞特性及再生治疗方法。
欢迎通过我们的免费点播课程学习人肠类器官的培养方法，或浏览关于使用 IntestiCult™ 进行类器官培养流程的常见问题（FAQs）。此外，您还可以下载我们的电子书《Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™》，获取肠类器官培养方案的精选合集。
如果您打算将本产品用于商业目的，请通过www.huborganoids.nl与HUB Organoids B.V.联系，以获取商业用途许可或HUB Organoids B.V.许可的相关说明。
在IntestiCult™ 类器官生长培养基 (人)中生长的类器官包含一个由具有极性的肠上皮细胞层包围而成的功能性腔体结构。为满足多样化的建模应用，这些类器官可在三维结构中，或在浸没式单层培养或气液界面（ALI）培养的二维结构中，通过 IntestiCult™ 类器官分化培养基（人）（产品号 #100-0214）实现进一步分化。
肠类器官培养的应用包括研究肠道上皮的发育和功能、构建肠道疾病模型，以及在肠道模型中筛选分子的有效性和毒性。肠类器官培养还可用于研究成体干细胞特性及再生治疗方法。
欢迎通过我们的免费点播课程学习人肠类器官的培养方法，或浏览关于使用 IntestiCult™ 进行类器官培养流程的常见问题（FAQs）。此外，您还可以下载我们的电子书《Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™》，获取肠类器官培养方案的精选合集。
如果您打算将本产品用于商业目的，请通过www.huborganoids.nl与HUB Organoids B.V.联系，以获取商业用途许可或HUB Organoids B.V.许可的相关说明。
亚型
专用培养基
细胞类型
肠道细胞
种属
人
应用
细胞培养，分化，扩增，培养，类器官培养
品牌
IntestiCult
研究领域
疾病建模，药物发现和毒理检测，上皮细胞研究，干细胞生物学
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (62)
文献 (26)
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice. Nature biotechnology 2023 6
Abstract
Human intestinal organoids (HIOs) derived from pluripotent stem cells provide a valuable model for investigating human intestinal organogenesis and physiology, but they lack the immune components required to fully recapitulate the complexity of human intestinal biology and diseases. To address this issue and to begin to decipher human intestinal-immune crosstalk during development, we generated HIOs containing immune cells by transplanting HIOs under the kidney capsule of mice with a humanized immune system. We found that human immune cells temporally migrate to the mucosa and form cellular aggregates that resemble human intestinal lymphoid follicles. Moreover, after microbial exposure, epithelial microfold cells are increased in number, leading to immune cell activation determined by the secretion of IgA antibodies in the HIO lumen. This in vivo HIO system with human immune cells provides a framework for future studies on infection- or allergen-driven intestinal diseases.
Catechol-O-Methyltransferase Loss Drives Cell-Specific Nociceptive Signaling via the Enteric Catechol-O-Methyltransferase/microRNA-155/Tumor Necrosis Factor ? Axis Gastroenterology 2023 4
Abstract
BACKGROUND & AIMS The etiology of abdominal pain in postinfectious, diarrhea-predominant irritable bowel syndrome (PI-IBS-D) is unknown, and few treatment options exist. Catechol-O-methyltransferase (COMT), an enzyme that inactivates and degrades biologically active catecholamines, plays an important role in numerous physiologic processes, including modulation of pain perception. Our objective was to determine the mechanism(s) of how decreased colonic COMT in PI-IBS-D patients contributes to the chronic abdominal pain phenotype after enteric infections. METHODS Colon neurons, epithelial cells, and macrophages were procured with laser capture microdissection from PI-IBS-D patients to evaluate cell-specific colonic COMT, microRNA-155 (miR-155), and tumor necrosis factor (TNF) ? expression levels compared to recovered patients (infection cleared: did not develop PI-IBS-D) and control individuals. COMT-/-, colon-specific COMT-/-, and miR-155-/- mice and human colonoids were used to model phenotypic expression of COMT in PI-IBS-D patients and to investigate signaling pathways linking abdominal pain. Citrobacter rodentium and trinitrobenzene sulfonic acid animal models were used to model postinflammatory changes seen in PI-IBS-D patients. RESULTS Colonic COMT levels were significantly decreased and correlated with increased visual analog scale abdominal pain ratings in PI-IBS-D patients compared to recovered patients and control individuals. Colonic miR-155 and TNF-? were increased in PI-IBS-D patients with diminished colonic COMT. COMT-/- mice had significantly increased expression of miR-155 and TNF-? in both colon tissues and dorsal root ganglia. Introduction of cV1q antibody (anti-TNF-?) into mice reversed visceral hypersensitivity after C rodentium and trinitrobenzene sulfonic acid. CONCLUSIONS Decreased colonic COMT in PI-IBS-D patients drives abdominal pain phenotypes via the COMT/miR-155/TNF-? axis. These important findings will allow new treatment paradigms and more targeted and personalized medicine approaches for gastrointestinal disorders after enteric infections.
3D bioprinted colorectal cancer models based on hyaluronic acid and signalling glycans. Carbohydrate polymers 2023 2
Abstract
In cancer microenvironment, aberrant glycosylation events of ECM proteins and cell surface receptors occur. We developed a protocol to generate 3D bioprinted models of colorectal cancer (CRC) crosslinking hyaluronic acid and gelatin functionalized with three signalling glycans characterized in CRC, 3'-Sialylgalactose, 6'-Sialylgalactose and 2'-Fucosylgalactose. The crosslinking, performed exploiting azide functionalized gelatin and hyaluronic acid and 4arm-PEG-dibenzocyclooctyne, resulted in biocompatible hydrogels that were 3D bioprinted with commercial CRC cells HT-29 and patient derived CRC tumoroids. The glycosylated hydrogels showed good 3D printability, biocompatibility and stability over the time. SEM and synchrotron radiation SAXS/WAXS analysis revealed the influence of glycosylation in the construct morphology, whereas MALDI-MS imaging showed that protein profiles of tumoroid cells vary with glycosylation, indicating that sialylation and fucosylation of ECM proteins induce diverse alterations to the proteome of the tumoroid and surrounding cells.
更多信息
| 种属 | Human |
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法律声明:
This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
