若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

MesenCult™ 成骨刺激试剂盒(小鼠)

小鼠间充质干细胞和胚胎成纤维细胞分化为成骨细胞的完全培养基

产品号 #(选择产品)

产品号 #05504_C

小鼠间充质干细胞和胚胎成纤维细胞分化为成骨细胞的完全培养基

产品优势

  • 本试剂盒可诱导扩增过的小鼠间充质干祖细胞和胚胎成纤维细胞发生强力的成骨分化作用,这体现在参与骨分化和成熟的关键成骨转录基因的高表达以及茜素红染色上。参见 “数据和出版物 ”产品页面分栏。

产品组分包括

  • MesenCult™ MSC 基础培养基(小鼠),200 mL;
  • MesenCult™ MSC成骨刺激补充剂(小鼠),50 mL

总览

MesenCult™成骨刺激试剂盒(小鼠)是专为来自致密骨和骨髓的小鼠间充质干祖细胞(MSCs)以及小鼠胚胎成纤维细胞(MEFs)分化为成骨细胞而研制。本试剂盒可诱导小鼠MSCs和 MEFs 强力成骨,这一点可通过对参与骨分化和成熟的关键转录基因进行 qPCR 分析和茜素红染色得到证明。本试剂盒推荐用于鉴定MSCs和 MEFs 以及研究骨发育。

亚型
专用培养基
 
细胞类型
间充质干/祖细胞,鼠胚胎成纤维细胞,成骨细胞
 
种属
小鼠
 
应用
细胞培养,分化
 
品牌
MesenCult
 
研究领域
干细胞生物学
 

实验数据

1 MOUSE COMPACT BONE-DERIVED MSC (CB-MSC) DATA<br>Compact bone-derived MSCs exhibited superior expression of key osteogenic transcripts involved in bone differentiation and maturation following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse)*

Figure 1. Differentiation of mouse compact bone-derived mesenchymal stem cells (CB-MSCs) with the MesenCult™ Osteogenic Stimulatory Kit (Mouse)

Compact bone-derived MSCs exhibited superior expression of key osteogenic transcripts involved in bone differentiation and maturation following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse)*

Compact bone-derived MSCs display robust bone matrix deposition following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse) as demonstrated by Alizarin Red-staining

Figure 2. Compact bone-derived MSCs display robust bone matrix deposition following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse) as demonstrated by Alizarin Red-staining

Ca2+ deposits are detected with Alizarin Red.
For both the qPCR analysis and Alizarin-Red staining assay, the mouse compact bone-derived MSCs were cultured in vitro for three passages, purified with EasySep™ Mesenchymal Stem/Progenitor Cell Enrichment Kit (Mouse, Catalog #19771), and then cultured for another passage, prior to differentiation with the MesenCult™ Osteogenic Stimulatory Kit (Mouse).

2 MOUSE EMBRYONIC FIBROBLASTS (MEF) DATA<br>MEFs exhibited superior expression of key osteogenic transcripts involved in bone differentiation and maturation following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse)*

Figure 3.Differentiation of mouse embryonic fibroblasts (MEFs) with MesenCult™ Osteogenic Stiumulatory Kit (Mouse)

MEFs exhibited superior expression of key osteogenic transcripts involved in bone differentiation and maturation following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse)*

MEFs treated with MesenCult™ Osteogenic Stimulatory Kit (Mouse) displayed substantially increased alkaline phosphatase activity and bone matrix deposition*

Figure 4. MEFs treated with MesenCult™ Osteogenic Stimulatory Kit (Mouse) displayed substantially increased alkaline phosphatase activity and bone matrix deposition*

Alkaline phosphatase activity is shown by Naphthol AS-MX Phosphate/Fast Red Violet LB Salt staining (red areas) while bone matrix deposition is shown by von Kossa staining (black areas).
*Versus a homemade osteogenic medium (mouse) from Lab A.
For the qPCR analysis and staining assays, MEFs were cultured in vitro for 20 days using MesenCult™ Osteogenic Stimulatory Kit (Mouse), homemade, or MesenCult™ Proliferation Kit (Mouse, Catalog #05511) as the negative control.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
05504
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
05504
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
05504
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (5)

文献 (6)

Radiation mitigation of the intestinal acute radiation injury in mice by 1-[(4-nitrophenyl)sulfonyl]-4-phenylpiperazine. S. Duhachek-Muggy et al. Stem cells translational medicine 2020 jan

Abstract

The objective of the study was to identify the mechanism of action for a radiation mitigator of the gastrointestinal (GI) acute radiation syndrome (ARS), identified in an unbiased high-throughput screen. We used mice irradiated with a lethal dose of radiation and treated with daily injections of the radiation mitigator 1-[(4-nitrophenyl)sulfonyl]-4-phenylpiperazine to study its effects on key pathways involved in intestinal stem cell (ISC) maintenance. RNASeq, quantitative reverse transcriptase-polymerase chain reaction, and immunohistochemistry were performed to identify pathways engaged after drug treatment. Target validation was performed with competition assays, reporter cells, and in silico docking. 1-[(4-Nitrophenyl)sulfonyl]-4-phenylpiperazine activates Hedgehog signaling by binding to the transmembrane domain of Smoothened, thereby expanding the ISC pool, increasing the number of regenerating crypts and preventing the GI-ARS. We conclude that Smoothened is a target for radiation mitigation in the small intestine that could be explored for use in radiation accidents as well as to mitigate normal tissue toxicity during and after radiotherapy of the abdomen.
Quercetin Exposure Suppresses the Inflammatory Pathway in Intestinal Organoids from Winnie Mice. M. Dicarlo et al. International journal of molecular sciences 2019 nov

Abstract

Inflammatory bowel diseases (IBDs) are chronic and relapsing immune disorders that result, or possibly originate, from epithelial barrier defects. Intestinal organoids are a new reliable tool to investigate epithelial response in models of chronic inflammation. We produced organoids from the ulcerative colitis murine model Winnie to explore if the chronic inflammatory features observed in the parental intestine were preserved by the organoids. Furthermore, we investigated if quercetin administration to in vitro cultured organoids could suppress LPS-induced inflammation in wild-type organoids (WT-organoids) and spontaneous inflammation in ulcerative colitis organoids (UC-organoids). Our data demonstrate that small intestinal organoids obtained from Winnie mice retain the chronic intestinal inflammatory features characteristic of the parental tissue. Quercetin administration was able to suppress inflammation both in UC-organoids and in LPS-treated WT-organoids. Altogether, our data demonstrate that UC-organoids are a reliable experimental system for investigating chronic intestinal inflammation and pharmacological responses.
The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis. B. Di Stefano et al. Cell stem cell 2019 nov

Abstract

Post-transcriptional mechanisms have the potential to influence complex changes in gene expression, yet their role in cell fate transitions remains largely unexplored. Here, we show that suppression of the RNA helicase DDX6 endows human and mouse primed embryonic stem cells (ESCs) with a differentiation-resistant, hyper-pluripotent" state which readily reprograms to a naive state resembling the preimplantation embryo. We further demonstrate that DDX6 plays a key role in adult progenitors where it controls the balance between self-renewal and differentiation in a context-dependent manner. Mechanistically DDX6 mediates the translational suppression of target mRNAs in P-bodies. Upon loss of DDX6 activity P-bodies dissolve and release mRNAs encoding fate-instructive transcription and chromatin factors that re-enter the ribosome pool. Increased translation of these targets impacts cell fate by rewiring the enhancer heterochromatin and DNA methylation landscapes of undifferentiated cell types. Collectively our data establish a link between P-body homeostasis chromatin organization and stem cell potency."

更多信息

更多信息
种属 Mouse
质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息,请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.