EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #05838_C
用于冻存STEMdiff™神经诱导培养基生成的神经祖细胞
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总览
STEMdiff™神经祖细胞冻存液不含血清，用于冻存人胚胎干细胞（ES）和诱导多能干细胞（iPS）衍生的神经祖细胞（NPCs）。该冻存液适用于冻存通过STEMdiff™神经诱导培养基（产品号 #05835）生成并在STEMdiff™神经祖细胞培养基（产品号 #05833）中培养的NPCs。NPCs可以在神经诱导后的任何时间点进行冻存，且具有可重复的高回收率。NPCs复苏后表现出健康的形态，表达NPC标志物并保留扩增和分化为神经元的潜力。
包含
• Dimethyl sulfoxide (DMSO)
• Other ingredients
细胞类型
神经细胞，PSC衍生，神经干/祖细胞，多能干细胞
种属
人
应用
冻存
品牌
STEMdiff
研究领域
疾病建模，神经科学，干细胞生物学
制剂类别
无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (7)
文献 (1)
Functionalizing Ascl1 with Novel Intracellular Protein Delivery Technology for Promoting Neuronal Differentiation of Human Induced Pluripotent Stem Cells Stem Cell Reviews and Reports 2016 AUG
Abstract
Pluripotent stem cells can become any cell type found in the body. Accordingly, one of the major challenges when working with pluripotent stem cells is producing a highly homogenous population of differentiated cells, which can then be used for downstream applications such as cell therapies or drug screening. The transcription factor Ascl1 plays a key role in neural development and previous work has shown that Ascl1 overexpression using viral vectors can reprogram fibroblasts directly into neurons. Here we report on how a recombinant version of the Ascl1 protein functionalized with intracellular protein delivery technology (Ascl1-IPTD) can be used to rapidly differentiate human induced pluripotent stem cells (hiPSCs) into neurons. We first evaluated a range of Ascl1-IPTD concentrations to determine the most effective amount for generating neurons from hiPSCs cultured in serum free media. Next, we looked at the frequency of Ascl1-IPTD supplementation in the media on differentiation and found that one time supplementation is sufficient enough to trigger the neural differentiation process. Ascl1-IPTD was efficiently taken up by the hiPSCs and enabled rapid differentiation into TUJ1-positive and NeuN-positive populations with neuronal morphology after 8 days. After 12 days of culture, hiPSC-derived neurons produced by Ascl1-IPTD treatment exhibited greater neurite length and higher numbers of branch points compared to neurons derived using a standard neural progenitor differentiation protocol. This work validates Ascl1-IPTD as a powerful tool for engineering neural tissue from pluripotent stem cells.
更多信息
| 种属 | Human |
|---|---|
| Contains | • Dimethyl sulfoxide (DMSO) • Other ingredients |
| 配方类别 | Serum-Free |
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