α-Ketoglutarate Accelerates the Initial Differentiation of Primed Human Pluripotent Stem Cells. TeSlaa T et al. Cell metabolism 2016 SEP
Abstract
Pluripotent stem cells (PSCs) can self-renew or differentiate from naive or more differentiated, primed, pluripotent states established by specific culture conditions. Increased intracellular α-ketoglutarate (αKG) was shown to favor self-renewal in naive mouse embryonic stem cells (mESCs). The effect of αKG or αKG/succinate levels on differentiation from primed human PSCs (hPSCs) or mouse epiblast stem cells (EpiSCs) remains unknown. We examined primed hPSCs and EpiSCs and show that increased αKG or αKG-to-succinate ratios accelerate, and elevated succinate levels delay, primed PSC differentiation. αKG has been shown to inhibit the mitochondrial ATP synthase and to regulate epigenome-modifying dioxygenase enzymes. Mitochondrial uncoupling did not impede αKG-accelerated primed PSC differentiation. Instead, αKG induced, and succinate impaired, global histone and DNA demethylation in primed PSCs. The data support αKG promotion of self-renewal or differentiation depending on the pluripotent state.
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they
can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our
privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
本产品系基于WiCell™研究院(WiCell™ Research Institute)知识产权授权许可开发。本产品的出售目的仅限于基于不可转让、用途受限之许可下的研究用途(无论购买者为学术机构或营利性主体)。购买本产品并不被授予为商业用途(即,以获利为目的的任何行为,如将本产品用于制造用途、或转售本产品或使用本产品所制成的任何材料、或将本产品或使用本产品所制成的材料用于提供服务)或临床应用(即,将本产品或本产品所用材料应用于人体)而出售、使用或另行转让本产品的权利,或出于基础临床前研究应用(包括但不限于畸胎瘤试验)以外的目的,由营利性主体或与其合作,将使用本产品制造的任何材料植入动物体内的权利。
购买者若不同意前述条款和条件,应保持产品完好,将其退还出售方以获退款。