技术资料

D. M. Previte et al. (apr 2019) Cell reports 27 1 129--141.e4

Lymphocyte Activation Gene-3 Maintains Mitochondrial and Metabolic Quiescence in Naive CD4+ T Cells.

Lymphocyte activation gene-3 (LAG-3) is an inhibitory receptor expressed by CD4+ T cells and tempers their homeostatic expansion. Because CD4+ T cell proliferation is tightly coupled to bioenergetics,we investigate the role of LAG-3 in modulating naive CD4+ T cell metabolism. LAG-3 deficiency enhances the metabolic profile of naive CD4+ T cells by elevating levels of mitochondrial biogenesis. In vivo,LAG-3 blockade partially restores expansion and the metabolic phenotype of wild-type CD4+ T cells to levels of Lag3-/- CD4+ T cells,solidifying that LAG-3 controls these processes. Lag3-/- CD4+ T cells also demonstrate greater signal transducer and activator of transcription 5 (STAT5) activation,enabling resistance to interleukin-7 (IL-7) deprivation. These results implicate this pathway as a target of LAG-3-mediated inhibition. Additionally,enhancement of STAT5 activation,as a result of LAG-3 deficiency,contributes to greater activation potential in these cells. These results identify an additional mode of regulation elicited by LAG-3 in controlling CD4+ T cell responses. View Publication

产品号#：

19852

19852RF

产品名：

EasySep™小鼠CD4+ T细胞分选试剂盒

RoboSep™ 小鼠CD4+ T细胞分选试剂盒
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FENS 2020
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仪器及软件

品牌：

Highway1
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Highway1
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Conference：

AAI 2025
技术公告
Simplify Your PBMC Isolations with the EasySep™ Direct Human PBMC Isolation Kit

细胞类型：

B细胞，NK细胞，T细胞，单个核细胞，单核细胞，淋巴细胞