技术资料

37:09

线上讲座
Quick and Easy Isolation of T Cells and Other Immune Cells from Large-Volume Samples

发布日期: 05/27/2022
实验方案
Optimizing Delivery Efficiency with Fluorescent Dextran Using the CellPore™ Transfection System

研究方向：

免疫学，疾病建模，药物发现和毒理检测
产品手册
Human Cord Blood Products Overview Flyer

品牌：

EasySep
6:05

视频
How to Thaw Frozen Human Primary Cells

发布日期: 11/13/2020
4:55

视频
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone

发布日期: 10/29/2020
技术窍门
Frequently Asked Questions on Primary Cells
29:54

线上讲座
ISSCR Innovation Showcase: Differentiating Immune Cells from Human Pluripotent Stem Cells in Feeder- and Serum-Free Cultures

发布日期: 08/17/2020
实验方案
How to Thaw Frozen Primary Cells

研究方向：

免疫学，干细胞生物学，疾病建模，药物发现和毒理检测，传染病，癌症研究
实验方案
如何从存放时间较长的血液样本去除粒细胞？

研究方向：

免疫学，药物发现和毒理检测，传染病
实验方案
如何通过免疫密度细胞分选技术分离出特定的细胞亚群？

研究方向：

免疫学，药物发现和毒理检测，传染病
实验方案
如何从全血制备白膜层？

研究方向：

免疫学，药物发现和毒理检测，传染病
Xu H et al. (JUL 2016) Organic & biomolecular chemistry 14 26 6179--83

Cellular thermal shift and clickable chemical probe assays for the determination of drug-target engagement in live cells.

Proof of drug-target engagement in physiologically-relevant contexts is a key pillar of successful therapeutic target validation. We developed two orthogonal technologies,the cellular thermal shift assay (CETSA) and a covalent chemical probe reporter approach (harnessing sulfonyl fluoride tyrosine labeling and subsequent click chemistry) to measure the occupancy of the mRNA-decapping scavenger enzyme DcpS by a small molecule inhibitor in live cells. Enzyme affinity determined using isothermal dose response fingerprinting (ITDRFCETSA) and the concentration required to occupy 50% of the enzyme (OC50) using the chemical probe reporter assay were very similar. In this case,the chemical probe method worked well due to the long offset kinetics of the reversible inhibitor (determined using a fluorescent dye-tagged probe). This work suggests that CETSA could become the first choice assay to determine in-cell target engagement due to its simplicity. View Publication

产品号#：

70025

70025.1

70025.2

70025.3

70047

70047.1

70047.2

70048

70048.1

70048.2

产品名：

冻存的人外周血单个核细胞

冻存的人外周血单个核细胞

冻存的人外周血单个核细胞

冻存的人外周血单个核细胞