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StemSpan™-AOF: HSPC 拡大培養用培地 (動物由来成分フリー、cGMP)

品牌：

StemSpan
1:03:04

线上讲座
Optimizing CD34+ Cell Genome Editing for Efficiency and HSPC Maintenance

发布日期: 05/30/2022
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技术公告
Potency Assays for Measuring the Engraftment Potential of HSPCs

细胞类型：

造血干/祖细胞
产品手册
StemSpan™–AOF: 以cGMP标准生产,用于细胞治疗研究

品牌：

StemSpan
实验方案
How to Prepare and Plate Semi-Solid Methylcellulose Medium for Cell Culture

研究方向：

干细胞生物学，细胞系制备，药物发现和毒理检测
Varela I et al. (DEC 2014) Cellular reprogramming 16 6 447--455

Generation of human &dollar;\&dollar;-thalassemia induced pluripotent cell lines by reprogramming of bone marrow-derived mesenchymal stromal cells using modified mRNA.

Synthetic modified mRNA molecules encoding pluripotency transcription factors have been used successfully in reprogramming human fibroblasts to induced pluripotent stem cells (iPSCs). We have applied this method on bone marrow-derived mesenchymal stromal cells (BM-MSCs) obtained from a patient with $$-thalassemia ($$-thal) with the aim to generate trangene-free $$-thal-iPSCs. Transfection of 10(4) BM-MSCs by lipofection with mRNA encoding the reprogramming factors Oct4,Klf4,Sox2,cMyc,and Lin28 resulted in formation of five iPSC colonies,from which three were picked up and expanded in $$-thal-iPSC lines. After 10 serial passages in vitro,$$-thal-iPSCs maintain genetic stability as shown by array comparative genomic hybridization (aCGH) and are capable of forming embryoid bodies in vitro and teratomas in vivo. Their gene expression profile compared to human embryonic stem cells (ESCs) and BM-MSCs seems to be similar to that of ESCs,whereas it differs from the profile of the parental BM-MSCs. Differentiation cultures toward a hematopoietic lineage showed the generation of CD34(+) progenitors up to 10%,but with a decreased hematopoietic colony-forming capability. In conclusion,we report herein the generation of transgene-free $$-thal-iPSCs that could be widely used for disease modeling and gene therapy applications. Moreover,it was demonstrated that the mRNA-based reprogramming method,used mainly in fibroblasts,is also suitable for reprogramming of human BM-MSCs. View Publication

产品号#：

04230

05850

05857

05870

05875

07923

60062

60062AD

60062AD.1

60062BT

60062FI

60062FI.1

60062PE

60062PE.1

85850

85857

85870

85875

05270

05275

产品名：

MethoCult™H4230

Dispase (1 U/mL)

Anti-Human SSEA-4 Antibody, Clone MC-813-70，Biotin

Anti-Human SSEA-4 Antibody, Clone MC-813-70，FITC

抗人SSEA-4抗体, Clone MC-813-70，FITC

Anti-Human SSEA-4 Antibody, Clone MC-813-70，PE

Anti-Human SSEA-4 Antibody, Clone MC-813-70，PE

mTeSR™1

mTeSR™1

STEMdiff™ APEL™2 培养基

STEMdiff™ APEL™2 培养基
1:52

视频
How to Prepare and Plate Semi-Solid Medium

发布日期: 03/02/2017