技术资料

8:00

视频
How to Use the CellPore™ Transfection System

发布日期: 12/04/2024
3:41

视频
Cryopreserve Human Peripheral Blood Mononuclear Cells with CryoStor® CS10

发布日期: 11/22/2024
1:54

视频
CellPore™: For Gentle Intracellular Delivery

发布日期: 10/10/2024
1:49

视频
How to Quickly Thaw Frozen Cells with the ThawStar® CFT2 Automated Thawing System

发布日期: 03/07/2023
产品手册
预富集CD138细胞制备杂交瘤

品牌：

ClonaCell
13:43

视频
How to Process a Leukopak for Downstream Cell Isolation
实验方案
Optimizing Delivery Efficiency with Fluorescent Dextran Using the CellPore™ Transfection System

研究方向：

免疫学，疾病建模，药物发现和毒性检测
6:05

视频
How to Thaw Frozen Human Primary Cells

发布日期: 11/13/2020
技术窍门
Frequently Asked Questions on Primary Cells
3:36

视频
Collaborating to Accelerate COVID-19 Research

发布日期: 05/15/2020
实验方案
How to Thaw Frozen Primary Cells

研究方向：

免疫学，干细胞生物学，疾病建模，药物发现和毒性检测，传染病，癌症研究
Alvarado G and Crowe JE ( 2016) 1442 63--76

Development of human monoclonal antibodies against respiratory syncytial virus using a high efficiency human hybridoma technique.

Human monoclonal antibodies against RSV have high potential for use as prophylaxis or therapeutic molecules,and they also can be used to define the structure of protective epitopes for rational vaccine design. In the past,however,isolation of human monoclonal antibodies was difficult and inefficient. Here,we describe contemporary methods for activation and proliferation of primary human memory B cells followed by cytofusion to non-secreting myeloma cells by dielectrophoresis to generate human hybridomas secreting RSV-specific monoclonal antibodies. We also provide experimental methods for screening human B cell lines to obtain RSV-specific lines,especially lines secreting neutralizing antibodies. View Publication

产品号#：

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产品名：

ClonaCell™-HY 杂交瘤试剂盒

ClonaCell™-HY Medium

ClonaCell™-HY Medium

ClonaCell™-HY Medium

ClonaCell™-HY Medium

ClonaCell™-HY Medium

ClonaCell™-HY PEG (融合)