技术资料

S. Bell et al. (JUL 2018) Stem cell reports 11 1 183--196

Disruption of GRIN2B Impairs Differentiation in Human Neurons.

Heterozygous loss-of-function mutations in GRIN2B,a subunit of the NMDA receptor,cause intellectual disability and language impairment. We developed clonal models of GRIN2B deletion and loss-of-function mutations in a region coding for the glutamate binding domain in human cells and generated neurons from a patient harboring a missense mutation in the same domain. Transcriptome analysis revealed extensive increases in genes associated with cell proliferation and decreases in genes associated with neuron differentiation,a result supported by extensive protein analyses. Using electrophysiology and calcium imaging,we demonstrate that NMDA receptors are present on neural progenitor cells and that human mutations in GRIN2B can impair calcium influx and membrane depolarization even in a presumed undifferentiated cell state,highlighting an important role for non-synaptic NMDA receptors. It may be this function,in part,which underlies the neurological disease observed in patients with GRIN2B mutations. View Publication

产品号#：

05833

05872

05873

05910

07174

05790

05792

05793

05794

05795

85850

85857

85870

85875

100-0483

100-0484

05914

100-0485

100-1077

产品名：

STEMdiff™神经前体细胞培养基

BrainPhys™神经元培养基

BrainPhys™神经元培养基和SM1试剂盒

BrainPhys™ 神经元培养基N2-A和SM1试剂盒

BrainPhys™原代神经元试剂盒

BrainPhys™ hPSC 神经元试剂盒

mTeSR™1

mTeSR™1

Hausser Scientificᵀᴹ 明线血球计数板

ReLeSR™

TeSR™-E7™重编程培养基（2组分）

温和细胞解离试剂

ReLeSR™
技术手册
Reversion and Maintenance of Human Naïve-Like Pluripotent Stem Cells with RSeT™ Medium
技术公告
Evaluation of Genome Editing

细胞类型：

T 细胞，多能干细胞，造血干/祖细胞
产品手册
检测人ES和IPS细胞中的核型异常

品牌：

TeSR
9:18

视频
How to Grow Cerebral Organoids from Human Pluripotent Stem Cells

发布日期: 07/19/2018
8:33

视频
How to Set Up an Assay with the hPSC Genetic Analysis Kit Experiment

发布日期: 07/18/2018
50:49

线上讲座
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling

发布日期: 07/13/2018
科学海报
A Feeder-Independent Culture System to Convert and Maintain Human Pluripotent Stem Cells in a Naïve-Like State

Conference：

ISSCR 2018
科学海报
Highly Efficient Differentiation of Human Pluripotent Stem Cells into Long-term Expandable “Mini-gut” Organoids
1:14:36

线上讲座
Re-Creating Disease with Kidney Organoids and CRISPR

发布日期: 07/12/2018
产品手册
qPCR Arrays for Cell Characterization

品牌：

MesenCult
挂图
Building Three-Dimensional Human Brain Organoids Overview of brain organogenesis and the applications of brain organoids in studying the development and maturation of the nervous system