EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72372_C
前列腺素通路激活剂；抑制 15-羟基 PGDH
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总览
16,16-二甲基前列腺素E2（16,16-二甲基PGE2）是15-羟基前列腺素脱氢酶（PGDH）的竞争性抑制剂 (North et al.; Ohno et al)。16,16-二甲基PGE2可作为大多数前列腺素E （EP）受体亚型的激动剂 (Coleman et al.; Robert et al.)，其活化Kd值约为1 nM（Coleman et al.）。16,16-二甲基PGE2以10 mg/mL (26 mM)的乙酸甲酯溶液形式提供。
维持和自我更新
·增加斑马鱼主动脉-生殖腺-中肾 (AGM) 区域和小鼠骨髓中的造血干细胞和祖细胞 (HSPC) 数量（North et al.）。
·介导 WNT 对斑马鱼 HSPC 自我更新的影响（Goessling et al.）。
细胞类型
造血干/祖细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
扩增
研究领域
干细胞生物学
CAS 编号
39746-25-3
化学式
C₂₂H₃₆O₅
纯度
≥ 95 %
通路
前列腺素衍生物
靶点
15-hydroxy PGDH
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (1)
Publications (5)
Genetic interaction of PGE2 and Wnt signaling regulates developmental specification of stem cells and regeneration. Cell 2009 MAR
Abstract
Interactions between developmental signaling pathways govern the formation and function of stem cells. Prostaglandin (PG) E2 regulates vertebrate hematopoietic stem cells (HSC). Similarly, the Wnt signaling pathway controls HSC self-renewal and bone marrow repopulation. Here, we show that wnt reporter activity in zebrafish HSCs is responsive to PGE2 modulation, demonstrating a direct interaction in vivo. Inhibition of PGE2 synthesis blocked wnt-induced alterations in HSC formation. PGE2 modified the wnt signaling cascade at the level of beta-catenin degradation through cAMP/PKA-mediated stabilizing phosphorylation events. The PGE2/Wnt interaction regulated murine stem and progenitor populations in vitro in hematopoietic ES cell assays and in vivo following transplantation. The relationship between PGE2 and Wnt was also conserved during regeneration of other organ systems. Our work provides in vivo evidence that Wnt activation in stem cells requires PGE2, and suggests the PGE2/Wnt interaction is a master regulator of vertebrate regeneration and recovery.
Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis. Nature 2007 JUN
Abstract
Haematopoietic stem cell (HSC) homeostasis is tightly controlled by growth factors, signalling molecules and transcription factors. Definitive HSCs derived during embryogenesis in the aorta-gonad-mesonephros region subsequently colonize fetal and adult haematopoietic organs. To identify new modulators of HSC formation and homeostasis, a panel of biologically active compounds was screened for effects on stem cell induction in the zebrafish aorta-gonad-mesonephros region. Here, we show that chemicals that enhance prostaglandin (PG) E2 synthesis increased HSC numbers, and those that block prostaglandin synthesis decreased stem cell numbers. The cyclooxygenases responsible for PGE2 synthesis were required for HSC formation. A stable derivative of PGE2 improved kidney marrow recovery following irradiation injury in the adult zebrafish. In murine embryonic stem cell differentiation assays, PGE2 caused amplification of multipotent progenitors. Furthermore, ex vivo exposure to stabilized PGE2 enhanced spleen colony forming units at day 12 post transplant and increased the frequency of long-term repopulating HSCs present in murine bone marrow after limiting dilution competitive transplantation. The conserved role for PGE2 in the regulation of vertebrate HSC homeostasis indicates that modulation of the prostaglandin pathway may facilitate expansion of HSC number for therapeutic purposes.
International Union of Pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes. Pharmacological reviews 1994 JUN
Abstract
更多信息
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Cas Number | 39746-25-3 |
| Chemical Formula | C₂₂H₃₆O₅ |
| 纯度 | ≥ 95% |
| Target | 15-hydroxy PGDH |
| Pathway | Prostanoid |
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