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抗β-微管蛋白III抗体，克隆AA10

抗人、小鼠、大鼠β - 球蛋白III的小鼠单克隆IgG2a抗体
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产品号 #60100_C

抗人、小鼠、大鼠β - 球蛋白III的小鼠单克隆IgG2a抗体

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

AA10抗体可与β-微管蛋白 III 发生反应，β-微管蛋白 III 是一种分子量约为 50 - 55 kDa 的结构蛋白，是微管蛋白的组成部分。微管蛋白是细胞骨架内微管的主要成分，由 α 和 β 微管蛋白亚基的异二聚体组装而成。β-微管蛋白 III 亚型，也称为神经元特异性 III 类 β-微管蛋白，主要在神经元中表达，并被广泛用作区分神经元与其他细胞类型的标记物。β-微管蛋白 III 参与神经元胞体和轴突中微管的形成，该功能涉及GTP结合，并在轴突运输、神经元细胞增殖和分化中发挥作用。它在多种癌症中高表达，是多种肿瘤的预测和预后标记物，例如，它存在于肿瘤细胞中，但在正常的神经胶质细胞中不存在。AA10 抗体预计可以识别所有哺乳动物 β-微管蛋白 III 同源物。

亚型
一抗
 
靶抗原
Beta-Tubulin III
 
别名
类 3 β-微管蛋白；类 III β-微管蛋白；MC1R；神经特异性类 3 β-微管蛋白；神经特异性类 III β-微管蛋白；TUBB 3；TUBB3；微管蛋白 β 3；微管蛋白 β 4；微管蛋白 β III
 
活性物种
人，小鼠，大鼠
 
偶联
Alexa Fluor 488，未偶联的
 
宿主物种
小鼠
 
细胞类型
神经细胞，PSC衍生，神经元
 
种属
人，小鼠，大鼠
 
应用
流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
神经科学
 
克隆
AA10
 
基因编号
10381
 
同种型
IgG2a，kappa
 

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实验数据

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Beta-Tubulin III Antibody, Clone AA10, followed by donkey anti-mouse IgG, Alexa Fluor® 488, and counter-stained with DAPI. Inset shows cells labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by donkey anti-mouse IgG, Alexa Fluor® 488, and counter-stained with DAPI. (B) Western blot analysis of denatured/reduced cell lysates from adult rat brain cortical cells (lane 1) or mouse E13.5 neural progenitor cells cultured with NeuroCult™ Proliferation Kit (Mouse) (negative control, lane 2) with Anti-Beta-Tubulin III Antibody, Clone AA10.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Beta-Tubulin III Antibody, Clone AA10, Alexa Fluor® 488 (green), and counterstained with DAPI (blue). Inset shows cells incubated with a mouse IgG2a, kappa Alexa Fluor® 488 isotype control antibody, and counterstained with DAPI.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Beta-Tubulin III Antibody, Clone AA10, Alexa Fluor® 488
Catalog #
60100AD.1, 60100AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Beta-Tubulin III Antibody, Clone AA10
Catalog #
60100.1, 60100
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Beta-Tubulin III Antibody, Clone AA10, Alexa Fluor® 488
Catalog #
60100AD.1, 60100AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Beta-Tubulin III Antibody, Clone AA10
Catalog #
60100.1, 60100
Lot #
All
Language
English

相关材料与文献

技术资料 (1)

Reliable Antibodies for Your Research
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Reliable Antibodies for Your Research

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更多信息
种属 Human, Mouse, Rat
克隆 AA10
Gene Id 10381
Alternative Names Class 3 beta-tubulin, class III beta-tubulin, MC1R, neuron-specific class 3 beta-tubulin, neuron-specific class III beta-tubulin, TUBB 3, TUBB3, tubulin beta 3, tubulin beta 4, tubulin beta III
同种型/isotype IgG2a, kappa
法律声明:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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