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抗β-微管蛋白III抗体,克隆AA10

抗人、小鼠、大鼠β - 球蛋白III的小鼠单克隆IgG2a抗体

产品号 #(选择产品)

产品号 #60100_C

抗人、小鼠、大鼠β - 球蛋白III的小鼠单克隆IgG2a抗体

总览

AA10抗体可与β-微管蛋白 III 发生反应,β-微管蛋白 III 是一种分子量约为 50 - 55 kDa 的结构蛋白,是微管蛋白的组成部分。微管蛋白是细胞骨架内微管的主要成分,由 α 和 β 微管蛋白亚基的异二聚体组装而成。β-微管蛋白 III 亚型,也称为神经元特异性 III 类 β-微管蛋白,主要在神经元中表达,并被广泛用作区分神经元与其他细胞类型的标记物。β-微管蛋白 III 参与神经元胞体和轴突中微管的形成,该功能涉及GTP结合,并在轴突运输、神经元细胞增殖和分化中发挥作用。它在多种癌症中高表达,是多种肿瘤的预测和预后标记物,例如,它存在于肿瘤细胞中,但在正常的神经胶质细胞中不存在。AA10 抗体预计可以识别所有哺乳动物 β-微管蛋白 III 同源物。

亚型
一抗
 
靶抗原
Beta-Tubulin III
 
别名
类 3 β-微管蛋白;类 III β-微管蛋白;MC1R;神经特异性类 3 β-微管蛋白;神经特异性类 III β-微管蛋白;TUBB 3;TUBB3;微管蛋白 β 3;微管蛋白 β 4;微管蛋白 β III
 
活性物种
人,小鼠,大鼠
 
偶联
Alexa Fluor 488,未偶联的
 
宿主物种
小鼠
 
细胞类型
神经细胞,PSC衍生,神经元
 
种属
人,小鼠,大鼠
 
应用
流式细胞术,免疫细胞化学,免疫荧光,免疫组化,免疫沉淀,Western印迹
 
研究领域
神经科学
 
克隆
AA10
 
基因编号
10381
 
同种型
IgG2a,kappa
 

实验数据

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Beta-Tubulin III Antibody, Clone AA10, followed by donkey anti-mouse IgG, Alexa Fluor® 488, and counter-stained with DAPI. Inset shows cells labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by donkey anti-mouse IgG, Alexa Fluor® 488, and counter-stained with DAPI. (B) Western blot analysis of denatured/reduced cell lysates from adult rat brain cortical cells (lane 1) or mouse E13.5 neural progenitor cells cultured with NeuroCult™ Proliferation Kit (Mouse) (negative control, lane 2) with Anti-Beta-Tubulin III Antibody, Clone AA10.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Beta-Tubulin III Antibody, Clone AA10, Alexa Fluor® 488 (green), and counterstained with DAPI (blue). Inset shows cells incubated with a mouse IgG2a, kappa Alexa Fluor® 488 isotype control antibody, and counterstained with DAPI.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60100AD.1, 60100AD
Lot #
All
Language
English
Catalog #
60100.1, 60100
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60100AD.1, 60100AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60100.1, 60100
Lot #
All
Language
English

相关材料与文献

技术资料 (1)

更多信息

更多信息
种属 Human, Mouse, Rat
克隆 AA10
Gene Id 10381
Alternative Names Class 3 beta-tubulin, class III beta-tubulin, MC1R, neuron-specific class 3 beta-tubulin, neuron-specific class III beta-tubulin, TUBB 3, TUBB3, tubulin beta 3, tubulin beta 4, tubulin beta III
同种型/isotype IgG2a, kappa
法律声明:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息,请访问 STEMCELL.CN/COMPLIANCE.
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