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抗GFAP抗体,克隆2E1.E9

抗人、小鼠、大鼠胶质纤维酸性蛋白(GFAP)的小鼠单克隆IgG2b抗体

产品号 #(选择产品)

产品号 #60048_C

抗人、小鼠、大鼠胶质纤维酸性蛋白(GFAP)的小鼠单克隆IgG2b抗体

总览

2E1.E9抗体可与胶质纤维酸性蛋白 (GFAP) 发生反应。GFAP是一种约 49 kDa的 III 型中间丝 (IF) 蛋白,在中枢神经系统中主要由星形胶质细胞表达,但在某些胶质细胞衍生的肿瘤中也发现其含量较高。GFAP被认为有助于细胞骨架的结构和强度。2E1.E9抗体不会与其他 IF 蛋白发生交叉反应,可用于区分星形胶质细胞与其他胶质细胞。GFAP也已在间质细胞、角质形成细胞、软骨细胞和骨细胞中被发现。GFAP多肽包含一个 N 端头部、一个中央杆状结构和一个 C 端尾部结构域,并通过依赖于 N 端结构域磷酸化和去磷酸化的过程组装成二聚体。目前已鉴定出几种剪接变体,编码三种不同的亚型。 GFAP基因的多种突变(>50)与亚历山大症 (Alexander disease) 有关,这是一种进行性白质脑病,其特征是细胞质内含体和髓鞘形成功能障碍。该抗体克隆已验证可用于标记在NeuroCult™ NS-A扩增试剂盒(人;产品号 #05751)和NeuroCult™扩增试剂盒(小鼠;产品号 #05702)中生长的神经干细胞和祖细胞。

亚型
一抗
 
靶抗原
GFAP (Glial Fibrillary Acidic Protein)
 
别名
胶质纤维酸性蛋白(GFAP)
 
活性物种
人,小鼠,大鼠
 
偶联
未偶联的
 
宿主物种
小鼠
 
细胞类型
星形胶质细胞,神经细胞,PSC衍生
 
种属
人,小鼠,大鼠
 
应用
流式细胞术,免疫细胞化学,免疫荧光,免疫组化,Western印迹
 
研究领域
神经科学
 
克隆
2E1.E9
 
基因编号
2670
 
同种型
IgG2b
 

实验数据

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-GFAP Antibody, Clone 2E1.E9, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG2b, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram). (B) Western blot analysis of denatured/reduced Sprague-Dawley rat brain lysate (lane 1) or HT1080 fibrosarcoma cells (negative control, lane 2) with Anti-GFAP Antibody, Clone 2E1.E9. (C) Embryonic mouse cortical tissue was cultured using the NeuroCult™ Proliferation Kit (Mouse), then fixed and labeled with Anti-GFAP Antibody, Clone 2E1.E9, followed by goat anti-mouse IgG, FITC. Nuclei were counter-stained with DAPI. Inset shows cells labeled with a mouse IgG2b, kappa isotype control antibody, followed by goat anti-mouse IgG, FITC (without DAPI staining).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60048.1, 60048
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60048.1, 60048
Lot #
All
Language
English

相关材料与文献

技术资料 (2)

文献 (1)

Onset of rosette formation during spontaneous neural differentiation of hESC and hiPSC colonies Malchenko S et al. Gene 2014 JAN

Abstract

In vitro neural differentiation of human embryonic stem cells (hESCs) is an advantageous system for studying early neural development. The process of early neural differentiation in hESCs begins by initiation of primitive neuroectoderm, which is manifested by rosette formation, with consecutive differentiation into neural progenitors and early glial-like cells. In this study, we examined the involvement of early neural markers - OTX2, PAX6, Sox1, Nestin, NR2F1, NR2F2, and IRX2 - in the onset of rosette formation, during spontaneous neural differentiation of hESC and human induced pluripotent stem cell (hiPSC) colonies. This is in contrast to the conventional way of studying rosette formation, which involves induction of neuronal differentiation and the utilization of embryoid bodies. Here we show that OTX2 is highly expressed at the onset of rosette formation, when rosettes comprise no more than 3-5 cells, and that its expression precedes that of established markers of early neuronal differentiation. Importantly, the rise of OTX2 expression in these cells coincides with the down-regulation of the pluripotency marker OCT4. Lastly, we show that cells derived from rosettes that emerge during spontaneous differentiation of hESCs or hiPSCs are capable of differentiating into dopaminergic neurons in vitro, and into mature-appearing pyramidal and serotonergic neurons weeks after being injected into the motor cortex of NOD-SCID mice. ?? 2013 Elsevier B.V.

更多信息

更多信息
种属 Human, Mouse, Rat
克隆 2E1.E9
Gene Id 2670
Alternative Names Glial fibrillary acid protein (GFAP)
同种型/isotype IgG2b
质量保证:

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