抗人CD117（c-Kit）抗体，克隆104D2

抗人、食蟹猴、牛CD117（c-Kit）的小鼠单克隆IgG1抗体

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产品号 #60087_C

抗人、食蟹猴、牛CD117（c-Kit）的小鼠单克隆IgG1抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

104D2抗体与CD117（c-Kit）反应。CD117是c-Kit配体（干细胞因子/钢因子）的1型跨膜受体，分子量约为145 kDa，广泛表达于骨髓造血干细胞，包括多能祖细胞、红系祖细胞、B淋巴细胞和T淋巴细胞前体细胞，以及肥大细胞和黑素细胞。CD117在约1 - 4%的骨髓细胞上表达，其中大多数（约50 - 70%）共表达CD34，包括祖细胞及其前体。CD117属于酪氨酸激酶受体家族，具有5个免疫球蛋白样C2型结构域和1个胞质蛋白激酶结构域。c-Kit配体与CD117结合可诱导二聚化和自磷酸化，从而激活几种对造血干细胞增殖和分化至关重要的细胞内信号通路。CD117信号传导通过磷酸酶和受体的快速内吞和降解来调控。104D2抗体的结合不会阻断c-Kit配体或抗体克隆SR1的结合。

亚型
一抗

靶抗原
CD117 (c-Kit)

别名
c-KIT，cKIT，干细胞因子受体（SCFR）

活性物种
Cow，Cynomolgus，人

偶联
APC，Biotin 或生物素，PE，未偶联的

宿主物种
小鼠

细胞类型
内胚层，PSC衍生，造血干/祖细胞

种属
人，非人灵长类，其它细胞系

应用
CyTOF，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀

研究领域
干细胞生物学

克隆
104D2

基因编号
3815

同种型
IgG1，kappa

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实验数据

Figure 1. Data for Unconjugated

Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human induced pluripotent stem (iPS) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (C) Flow cytometry analysis of human embryonic stem (ES) cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC and Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE (Catalog #60089PE). The emergence of definitive endoderm can be monitored by co-expression of CD117 and CD184. (D) Flow cytometry analysis of human ES cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with a mouse IgG1, kappa APC isotype control antibody and Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE).

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC (solid line histogram).

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).