抗人CD14抗体，克隆M5E2

抗人、恒河猴、食蟹猴CD14的小鼠单克隆IgG2a抗体

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产品号 #60004_C

抗人、恒河猴、食蟹猴CD14的小鼠单克隆IgG2a抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

M5E2抗体可识别CD14，这是一种分子量约53-55 kDa的GPI锚定跨膜糖蛋白，在外周血单核细胞和巨噬细胞表面高表达，在粒细胞中表达水平较低。单核细胞/巨噬细胞与粒细胞之间约10倍的表达差异使得CD14成为区分这两类细胞群的有效标志物。CD14也表达于组织的巨噬细胞、朗格汉斯细胞和树突状细胞。CD14作为脂多糖（LPS）与血清LPS结合蛋白复合物的高亲和力受体，通过与TLR4和MD-2形成共受体，来调节革兰氏阴性菌感染免疫应答中的LPS依赖性信号转导，进而触发NF-κB活化、细胞因子分泌及炎症反应诱导。此外，已报道的CD14可溶性形式包括约48 kDa和55 kDa两种。

该抗体克隆（M5E2）已通过验证，可用于EasySep™分选试剂盒分离细胞的纯度检测，包括 EasySep™ 人髓系正选试剂盒（产品号 #18653）和 EasySep™ 人CD14正选试剂盒（产品号 #18058）；可能观察到部分阻断，以及 EasySep™ HLA 全血 CD33 正选试剂盒（产品号 #18287HLA）、EasySep™ HLA 全血髓系正选试剂盒（产品号 #18683HLA）和 EasySep™ 人白膜层 CD14 正选试剂盒（产品号 #18088）。

亚型
一抗

靶抗原
CD14

别名
LPS 受体

活性物种
Capuchin Monkey，Chimpanzee，Common Marmoset，Cotton-Topped Tamarin，Cow，Cynomolgus，Dog，人，Macaque，Pig，Rhesus，Sheep，Squirrel Monkey

偶联
Alexa Fluor 488，APC，Biotin 或生物素，FITC，PE，未偶联的

宿主物种
小鼠

细胞类型
单核细胞

种属
人，非人灵长类，其它细胞系

应用
CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，中和及阻断

研究领域
免疫

克隆
M5E2

基因编号
929

同种型
IgG2a，kappa

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实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488 and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AD). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD14 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) is shown (solid line histogram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 is shown (solid line histogram).

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ; solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC is shown (solid line histogram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, APC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC is shown (solid line histogram).

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, Biotin followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC (solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin followed by SAV APC is shown (solid line histogram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin followed by SAV APC is shown (solid line histogram).

Figure 4. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC (Catalog #60071FI; solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC is shown (solid line historgram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC is shown (solid line histogram).

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, PE (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD14 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) is shown (solid line historgram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE is shown (solid line histogram).

Figure 6. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).