抗人CD20抗体，克隆2H7

小鼠单克隆IgG2b抗体，抗人、恒河猴、食蟹猴CD20

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产品号 #60008_C

小鼠单克隆IgG2b抗体，抗人、恒河猴、食蟹猴CD20

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总览

实验数据

产品说明书及文档

应用领域

总览

2H7抗体识别CD20，CD20是MS4A蛋白家族中一个~35 kDa的非糖基化1型跨膜蛋白。CD20多肽穿过细胞膜四次，只有一小部分蛋白外露在细胞表面。抗体2H7识别的表位已被定位到位于CD20胞外大环的氨基酸序列YNCEPANPSEKNSPST。CD20在pre-B细胞、静息和活化的B细胞、一些滤泡树突状细胞和一些T细胞亚群上表达。B细胞在分化为浆细胞时会失去CD20表达。通过与包括B细胞受体（CD79）、MHC I类和II类、CD53、CD81和CD82在内的多种蛋白结合，CD20参与启动调控B细胞激活、增殖和分化的细胞内信号通路。CD20被认为形成多亚基离子通道，调节钙离子在质膜上的通量。CD20的激活伴随着~33 kDa载脂蛋白胞质域的明显磷酸化，出现35 - 37 kDa异构体，与Src家族激酶如Fyn、Lck和Lyn相关。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™人全血CD20正选试剂盒（产品号 #18685），EasySep™人CD19正选试剂盒II（产品号 #17854）和EasySep™HLA全血淋巴细胞正选试剂盒（产品号 #18684HLA））分选的细胞纯度。

亚型
一抗

靶抗原
CD20

别名
B细胞抗原，B1，Bp35，Leu-16，MS4A1

活性物种
Baboon，Capuchin Monkey，Chimpanzee，Cynomolgus，人，Nancy Ma's Night Monkey，Pigtailed Macaque，Rhesus，Squirrel Monkey

偶联
Alexa Fluor 488，APC，Biotin 或生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的

宿主物种
小鼠

细胞类型
B 细胞

种属
人，非人灵长类

应用
细胞分选，CyTOF，ELISAs，流式细胞术，免疫组化，免疫沉淀

研究领域
嵌合体，HLA，免疫，移植研究

克隆
2H7

基因编号
931

同种型
IgG2b，kappa

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实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488 and anti-human CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488. Histograms show labeling of the PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human buffy coat cells labeled with Anti-Human CD20 Antibody, Clone 2H7, APC (filled histogram) or a mouse IgG2b, kappa APC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD20 Antibody, Clone 2H7, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG2b, kappa APC isotype control antibody is shown (open histogram).

Figure 3. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, FITC (filled histogram) or a mouse IgG2b, kappa FITC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD20 Antibody, Clone 2H7, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG2b, kappa FITC isotype control antibody is shown (open histogram).

Figure 4. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, PE and antihuman CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD20 Antibody, Clone 2H7, PE. Histograms show labeling of the PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).

Figure 5. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11 (Catalog #60072), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Figure 6. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin (Catalog #60072BT), followed by SAV APC (solid line histogram).

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, PerCP-Cy5.5 (filled histogram) or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PerCP-Cy5.5 (Catalog #60072PS; solid line histogram).