Anti-Human CD63 Antibody, Clone H5C6

总览

克隆H5C6抗体可识别人类CD63分子的胞外表位。CD63是一种30-60 kDa的III型溶酶体糖蛋白，属于四跨膜蛋白超家族成员。该蛋白表达广泛，存在于单核细胞、巨噬细胞、激活的嗜碱性粒细胞、成纤维细胞、平滑肌细胞及激活的血小板表面。CD63主要定位于晚期内体、溶酶体和分泌囊泡中，并在这些区室间循环转运，同时也是细胞外囊泡的特征性标志物。

四跨膜蛋白（如CD63）具有四个跨膜结构域、两个胞外环及较短的胞内N端和C端。CD63能与多种整合素、共受体及其他蛋白结合，在质膜上形成称为"四跨膜蛋白富集微域"的多分子复合物。该蛋白参与细胞活化、粘附、分化及肿瘤侵袭等多种生理过程。研究表明，CD63与肿瘤进展密切相关，其蛋白缺陷可导致Hermansky-Pudlak综合征（一种以血小板功能障碍和溶酶体贮积缺陷为特征的罕见常染色体隐性遗传病）。

亚型
一抗

靶抗原
CD63

别名
gp55

活性物种
African Green Monkey，Baboon，Capuchin Monkey，Chimpanzee，Cynomolgus，人，Rhesus

偶联
未偶联的

宿主物种
小鼠

细胞类型
其它细胞系

应用
流式细胞术，Western印迹

研究领域
细胞外囊泡研究

克隆
H5C6

基因编号
967

同种型
IgG1，kappa

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实验数据

Data showing western blot analysis of extracellular vesicles isolated from mesenchymal stromal cell-conditioned medium with Anti-Human CD63 Antibody, Clone H5C6 and flow cytometry analysis of human peripheral blood mononuclear cells labeled with Anti-Human CD63 Antibody, Clone H5C6 .

Figure 1. Analysis of Anti-Human CD63 Antibody, Clone H5C6 with Western Blot and Flow Cytometry

Extracellular vesicles (EVs) were isolated from mesenchymal stromal cell (MSC)-conditioned medium using a 2 mL EV size exclusion chromatography column and analysed with (A) western blot analysis using Anti-Human CD63 Antibody, Clone H5C6. Lanes (left to right) were loaded with isolated fractions 9, 10, and 11, respectively. Further validation was performed by flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; viable lymphocytes gated for analysis) stimulated with phorbol myristate acetate (PMA) and ionomycin, then (B) labeled with Anti-Human CD63 Antibody, Clone H5C6, followed by a goat anti-mouse IgG1 antibody, APC (filled histogram), or Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC (solid line histogram), or (C) labeled with Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC, or (D) labeled with Anti-Human CD63 Antibody, Clone H5C6, followed by a goat anti-mouse IgG1 antibody, APC.