抗人 CD81 (TAPA-1) 抗体，克隆 5A6

小鼠单克隆抗体，抗人、恒河猴、食蟹猴CD81，未偶联

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产品号 #100-0209_C

小鼠单克隆抗体，抗人、恒河猴、食蟹猴CD81，未偶联

总览

实验数据

产品说明书及文档

应用领域

总览

5A6 抗体与位于人 CD81（TAPA-1）的大细胞外环（EC2）的表位发生反应。CD81 的表达范围很广，包括 B 细胞、T 细胞、NK 细胞、单核细胞、树突状细胞、胸腺细胞、内皮细胞和成纤维细胞的表面。它也是细胞外囊泡的特征标志物。

CD81 是一种 26 kDa 的非糖基化 III 型跨膜蛋白，属于四跨蛋白（Tetraspanin）家族。Tetraspanin包含四个跨膜结构域、两个胞外环以及短的胞质 N 端和 C 端。CD81 与许多整合素、共受体和其他蛋白结合，在质膜上形成多分子复合物，称为四跨蛋白富集微域。近膜半胱氨酸残基的棕榈酰化对其与其他蛋白和质膜的结合非常重要。

CD81 可调节多种生物功能，包括细胞粘附和融合、细胞迁移和增殖。例如，CD81 与 CD19 和 CD21 一起构成 B 细胞上 B 细胞受体的共受体。当 CD81 与 CD19 复合时，5A6 抗体的表位被隐藏起来，但当 B 细胞被激活后，5A6 抗体的表位就可以被暴露。CD81突变与普通变异性免疫缺陷症（CVID）有关。CD81 还与介导丙型肝炎病毒进入细胞有关。

分类
一抗

靶抗原
CD81

别名
CVID6

反应种属
非洲绿猴、狒狒、黑猩猩、食蟹猴、人、恒河猴

偶联
未偶联

宿主物种
小鼠

细胞类型
其它细胞系

应用
流式细胞术，Western印迹

研究领域
细胞外囊泡研究

克隆
5A6

Gene ID
975

Isotype
IgG1, κ轻链

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实验数据

Data showing western blot analysis of extracellular vesicles isolated from mesenchymal stromal cell-conditioned medium with Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6 and flow cytometry analysis of human peripheral blood mononuclear cells labeled with Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6.

Figure 1. Analysis of Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6 with Western Blot and Flow Cytometry

Extracellular vesicles (EVs) isolated from mesenchymal stromal cell (MSC)-conditioned medium using a 2 mL EV size exclusion chromatography column were analysed with (A) western blot analysis with Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6. Lanes (left to right) were loaded with isolated fractions 9, 10, and 11, respectively. Further validation was performed by flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; viable lymphocytes were gated for analysis) stimulated with phorbol myristate acetate (PMA) and ionomycin and then (B) labeled with Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6, followed by a goat anti-mouse IgG1 antibody, APC (filled histogram), or Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC (solid line histogram), or (C) labeled with Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC, or (D) labeled with Anti-Human CD81 (TAPA-1) Antibody, Clone 5A6, followed by a goat anti-mouse IgG1 antibody, APC.