抗人 CD9 抗体，克隆 HI9a

总览

HI9a 抗体与人 CD9（一种 24 kDa 单链 III 型跨膜糖蛋白）上的细胞外表位发生反应。CD9 广泛表达于单核细胞、发育中的 B 细胞、活化的 T 细胞、粒细胞、上皮细胞、内皮细胞和血小板（主要是 α 颗粒）的表面，也是细胞外囊泡的特征的标志物。

CD9 属于四跨蛋白（tetraspanin）家族，包含四个跨膜结构域、两个细胞外环以及短的细胞质 N 端和 C 端。它通过促进细胞粘附、迁移和信号转导，参与介导包括血管生成在内的多个细胞过程，并参与与受精、破骨细胞生成和肌生成有关的细胞融合过程。

亚型
一抗

靶抗原
CD9

别名
DRAP-24

活性物种
African Green Monkey，Baboon，Capuchin Monkey，Chimpanzee，Cynomolgus，人，Rhesus

偶联
未偶联的

宿主物种
小鼠

细胞类型
其它细胞系

应用
流式细胞术，Western印迹

研究领域
细胞外囊泡研究

克隆
HI9a

基因编号
928

同种型
IgG1，kappa

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实验数据

Data showing western blot analysis of extracellular vesicles isolated from mesenchymal stromal cell-conditioned medium with Anti-Human CD9 Antibody, Clone HI9a and flow cytometry analysis of human peripheral blood mononuclear cells labeled with Anti-Human CD9 Antibody, Clone HI9a.

Figure 1. Analysis of Anti-Human CD9 Antibody, Clone HI9a with Western Blot and Flow Cytometry

Extracellular vesicles (EVs) were isolated from mesenchymal stromal cell (MSC)-conditioned medium using a 2 mL EV size exclusion chromatography column and analyzed by (A) western blot with Anti-Human CD9 Antibody, Clone HI9a. Lanes (left to right) were loaded with isolated fractions 9, 10, and 11, respectively. Further validation was performed by flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; viable lymphocytes gated for analysis) stimulated with phorbol myristate acetate (PMA) and ionomycin and then (B) labeled with Anti-Human CD9 Antibody, Clone HI9a, followed by a goat anti-mouse IgG1 antibody, APC (filled histogram), or Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC (solid line histogram), or (C) labeled with Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC, or (D) labeled with Anti-Human CD9 Antibody, Clone HI9a, followed by a goat anti-mouse IgG1 antibody, APC.