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抗人p63(deltaN)抗体,克隆Poly6190

抗人p63(deltaN)的兔多克隆抗体

产品号 #(选择产品)

产品号 #60154_C

抗人p63(deltaN)的兔多克隆抗体

总览

Poly6190抗体可特异性识别人源p63蛋白的deltaN亚型。p63作为p53转录因子家族成员,通过可变剪接产生两类功能迥异的亚型。TA亚型(TA p63-α、-β、-γ)具有N端转录激活域,而deltaN亚型(deltaN p63-α、-β、-γ)缺失转录激活域。TA亚型能够激活p53靶基因转录,同时拮抗p53蛋白功能。deltaN亚型通常对TA亚型具有显性负调控作用,抑制其功能。p63蛋白位于细胞核内,在上皮干细胞再生、表皮形态发生、肢体发育和上皮分层中发挥重要作用。

亚型
一抗
 
靶抗原
p63 (deltaN)
 
别名
TP63;肿瘤蛋白 P63
 
活性物种

 
偶联
未偶联的
 
宿主物种

 
细胞类型
气道细胞,真皮细胞
 
应用
免疫细胞化学,免疫荧光,免疫组化,Western印迹
 
研究领域
上皮细胞研究,干细胞生物学
 
克隆
Not applicable (poly6190)
 
基因编号
8626
 
同种型
IgG
 

实验数据

(A) Primary human airway epithelial cells were expanded in a submerged culture with PneumaCult™-Ex Medium (Catalog #05008) and labeled with Anti- Human p63 (deltaN) Antibody, Clone Poly6190, followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594. (B) DAPI counterstaining of the cells shown in figure (A). (C) Primary human airway epithelial cells cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface were cryo-sectioned and labeled with Anti-Human p63 (deltaN) Antibody, Clone Poly6190 (red), followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594 (red), and antihuman NGF Receptor/p75NTR (CD271) antibody, followed by a donkey anti-mouse IgG antibody, Alexa Fluor® 488 (green). (D) Western blot analysis of non-transfected HEK293 cell lysates (negative control; lane 1) and p63 (deltaN)-transfected HEK293 cell lysates (lane 2). Lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with Anti-Human p63 (deltaN) Antibody, Clone Poly6190. Proteins were visualized using an HRP-conjugated donkey anti-rabbit IgG secondary antibody and chemiluminescence detection.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60154
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60154
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (2)

更多信息

更多信息
克隆 Not applicable (poly6190)
Gene Id 8626
Alternative Names TP63, Tumor protein P63
同种型/isotype IgG
法律声明:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息,请访问 STEMCELL.CN/COMPLIANCE.
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