抗人p63（deltaN）抗体，克隆Poly6190

抗人p63（deltaN）的兔多克隆抗体

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产品号 #（选择产品）

产品号 #60154_C

抗人p63（deltaN）的兔多克隆抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

Poly6190抗体可特异性识别人源p63蛋白的deltaN亚型。p63作为p53转录因子家族成员，通过可变剪接产生两类功能迥异的亚型。TA亚型（TA p63-α、-β、-γ）具有N端转录激活域，而deltaN亚型（deltaN p63-α、-β、-γ）缺失转录激活域。TA亚型能够激活p53靶基因转录，同时拮抗p53蛋白功能。deltaN亚型通常对TA亚型具有显性负调控作用，抑制其功能。p63蛋白位于细胞核内，在上皮干细胞再生、表皮形态发生、肢体发育和上皮分层中发挥重要作用。

亚型
一抗

靶抗原
p63 (deltaN)

别名
TP63；肿瘤蛋白 P63

活性物种
人

偶联
未偶联的

宿主物种
兔

细胞类型
气道细胞，真皮细胞

应用
免疫细胞化学，免疫荧光，免疫组化，Western印迹

研究领域
上皮细胞研究，干细胞生物学

克隆
Not applicable (poly6190)

基因编号
8626

同种型
IgG

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实验数据

(A) Primary human airway epithelial cells were expanded in a submerged culture with PneumaCult™-Ex Medium (Catalog #05008) and labeled with Anti- Human p63 (deltaN) Antibody, Clone Poly6190, followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594. (B) DAPI counterstaining of the cells shown in figure (A). (C) Primary human airway epithelial cells cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface were cryo-sectioned and labeled with Anti-Human p63 (deltaN) Antibody, Clone Poly6190 (red), followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594 (red), and antihuman NGF Receptor/p75NTR (CD271) antibody, followed by a donkey anti-mouse IgG antibody, Alexa Fluor® 488 (green). (D) Western blot analysis of non-transfected HEK293 cell lysates (negative control; lane 1) and p63 (deltaN)-transfected HEK293 cell lysates (lane 2). Lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with Anti-Human p63 (deltaN) Antibody, Clone Poly6190. Proteins were visualized using an HRP-conjugated donkey anti-rabbit IgG secondary antibody and chemiluminescence detection.