产品号 #60154_C
抗人p63(deltaN)的兔多克隆抗体
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Poly6190抗体可特异性识别人源p63蛋白的deltaN亚型。p63作为p53转录因子家族成员,通过可变剪接产生两类功能迥异的亚型。TA亚型(TA p63-α、-β、-γ)具有N端转录激活域,而deltaN亚型(deltaN p63-α、-β、-γ)缺失转录激活域。TA亚型能够激活p53靶基因转录,同时拮抗p53蛋白功能。deltaN亚型通常对TA亚型具有显性负调控作用,抑制其功能。p63蛋白位于细胞核内,在上皮干细胞再生、表皮形态发生、肢体发育和上皮分层中发挥重要作用。
亚型
一抗
靶抗原
p63 (deltaN)
别名
TP63;肿瘤蛋白 P63
活性物种
人
偶联
未偶联的
宿主物种
兔
细胞类型
气道细胞,真皮细胞
应用
免疫细胞化学,免疫荧光,免疫组化,Western印迹
研究领域
上皮细胞研究,干细胞生物学
克隆
Not applicable (poly6190)
基因编号
8626
同种型
IgG
(A) Primary human airway epithelial cells were expanded in a submerged culture with PneumaCult™-Ex Medium (Catalog #05008) and labeled with Anti- Human p63 (deltaN) Antibody, Clone Poly6190, followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594. (B) DAPI counterstaining of the cells shown in figure (A). (C) Primary human airway epithelial cells cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface were cryo-sectioned and labeled with Anti-Human p63 (deltaN) Antibody, Clone Poly6190 (red), followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594 (red), and antihuman NGF Receptor/p75NTR (CD271) antibody, followed by a donkey anti-mouse IgG antibody, Alexa Fluor® 488 (green). (D) Western blot analysis of non-transfected HEK293 cell lysates (negative control; lane 1) and p63 (deltaN)-transfected HEK293 cell lysates (lane 2). Lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with Anti-Human p63 (deltaN) Antibody, Clone Poly6190. Proteins were visualized using an HRP-conjugated donkey anti-rabbit IgG secondary antibody and chemiluminescence detection.
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克隆 | Not applicable (poly6190) |
---|---|
Gene Id | 8626 |
Alternative Names | TP63, Tumor protein P63 |
同种型/isotype | IgG |
在气液界面培养的人气道上皮细胞的无血清和无bpe培养基
无血清和无bpe培养基用于原代人气道上皮细胞的扩增
小鼠单克隆IgG1抗体,抗大鼠NGFR/p75NTR (CD271)
抗人MUC1(CD227)的小鼠单克隆IgG1抗体
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