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抗小鼠CD11b抗体，克隆M1/70

抗人、小鼠、恒河猴CD11b的大鼠单克隆IgG2b抗体
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产品号 #60001_C

抗人、小鼠、恒河猴CD11b的大鼠单克隆IgG2b抗体

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

M1/70抗体可特异性识别CD11b，CD11b是一种分子量约170 kDa的1型跨膜糖蛋白，与CD18非共价结合形成异二聚体Mac-1受体。Mac-1通过结合ICAM-1（CD54）、ICAM-2（CD102）、ICAM-4（CD242）、iC3b和纤维蛋白原等配体，在多个过程中发挥作用，包括介导中性粒细胞/单核细胞与活化内皮细胞的粘附以及促进补体包被颗粒的吞噬作用。CD11b表达于粒细胞、单核细胞、NK细胞、树突状细胞、组织巨噬细胞以及T细胞和B细胞亚群的表面，并被用作区分naïve CD8+ T细胞和记忆CD8+ T细胞的标志物。CD11b是一个相对较晚的髓系分化标志物，在大多数髓系单核细胞造血祖细胞和更初始的细胞中检测不到。研究发现，M1/70抗体可抑制iC3b与Mac-1的结合。该抗体克隆已验证可用于通过EasySep™试剂盒（包括EasySep™小鼠单核细胞分选试剂盒，产品号 #19861）分离的细胞的纯度检测。

亚型
一抗
 
靶抗原
CD11b
 
别名
alphaM整合素，C3biR，CR3，Ly-40，Mac-1，Mo1
 
活性物种
Baboon，Chimpanzee，人，兔，Rhesus
 
偶联
Biotin 或 生物素，FITC，PE，未偶联的
 
宿主物种
大鼠
 
细胞类型
粒细胞及其亚群，单核细胞
 
种属
人，小鼠，非人灵长类，其它细胞系
 
应用
细胞分选，CyTOF，FACS，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，放射免疫分析，Western印迹
 
研究领域
免疫
 
克隆
M1/70
 
基因编号
16409
 
同种型
IgG2b，kappa
 

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实验数据

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488 and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody and anti-mouse CD45 APC.
(C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).
(D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488. Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa PE isotype control antibody and anti-mouse CD45 APC.
(C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).
(D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE. Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).

Data for unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD11b Antibody, Clone M1/70, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa APC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa biotin isotype control antibody followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa FITC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 8. Data for PB-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488
Catalog #
60001AD, 60001AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, APC
Catalog #
60001AZ, 60001AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Biotin
Catalog #
100-0439, 100-0438, 60001BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, FITC
Catalog #
100-0437, 100-0436, 60001FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™
Catalog #
60001PB, 60001PB.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PE
Catalog #
100-0435, 100-0434
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5
Catalog #
60001PS, 60001PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70
Catalog #
100-0433
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488
Catalog #
60001AD, 60001AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, APC
Catalog #
60001AZ, 60001AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Biotin
Catalog #
100-0439, 100-0438, 60001BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, FITC
Catalog #
100-0437, 100-0436, 60001FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™
Catalog #
60001PB, 60001PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PE
Catalog #
100-0435, 100-0434
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5
Catalog #
60001PS, 60001PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70
Catalog #
100-0433
Lot #
All
Language
English

更多信息

更多信息
种属 Human, Mouse, Non-Human Primate, Other
克隆 M1/70
Gene Id 16409
Alternative Names alphaM integrin, C3biR, CR3, Ly-40, Mac-1, Mo1
同种型/isotype IgG2b, kappa
法律声明:

Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. 质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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