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抗小鼠CD24抗体，克隆M1/69

大鼠单克隆IgG2b抗体，抗小鼠CD24
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产品号 #（选择产品）

产品号 #60099_C

大鼠单克隆IgG2b抗体，抗小鼠CD24

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

M1/69抗体识别小鼠CD24，CD24是一种表达在淋巴细胞、粒细胞、胸腺细胞、红细胞、上皮细胞、神经元和树突状细胞上的~35 - 45 kDa糖蛋白。CD24在T细胞和B细胞发育过程中存在差异表达，在大多数成熟的外周T细胞或浆细胞中均未发现其表达。它在pro-B、pre-B和成熟B细胞上表达，但在B细胞活化后表达大幅下调。CD24参与调节B细胞凋亡，并参与阻止B细胞终末分化为分泌抗体的浆母细胞和浆细胞。CD24作为一种粘附或共刺激分子，通过嗜同相互作用或与P-选择素（CD62P）等配体结合，参与淋巴细胞的活化、增殖和分化。

亚型
一抗
 
靶抗原
CD24
 
别名
CD24a，热稳定抗原，HSA，Ly-52，Nectadrin，小细胞肺癌簇4抗原
 
活性物种
小鼠
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
大鼠
 
细胞类型
B 细胞，乳腺细胞
 
种属
小鼠
 
应用
CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，Western印迹
 
研究领域
上皮细胞研究，免疫
 
克隆
M1/69
 
基因编号
12484
 
同种型
IgG2b，kappa
 

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实验数据

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, Alexa Fluor® 488 (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC (solid line histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, PE (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (Catalog #60077PE) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Data for APC-Conjugated

Figure 6. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Data for FITC-Conjugated

Figure 7. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, Alexa Fluor® 488
Catalog #
60099AD, 60099AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, APC
Catalog #
60099AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, Biotin
Catalog #
60099BT.1, 60099BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, PE
Catalog #
60099PE, 60099PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, PerCP-Cy5.5
Catalog #
60099PS, 60099PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69
Catalog #
60099.1, 60099
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, Alexa Fluor® 488
Catalog #
60099AD, 60099AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, APC
Catalog #
60099AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, Biotin
Catalog #
60099BT.1, 60099BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, PE
Catalog #
60099PE, 60099PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69, PerCP-Cy5.5
Catalog #
60099PS, 60099PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD24 Antibody, Clone M1/69
Catalog #
60099.1, 60099
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

文献 (1)

Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function Pond AC et al. Stem cells (Dayton, Ohio) 2013

Abstract

Fibroblast growth factor (FGF) signaling plays an important role in embryonic stem cells and adult tissue homeostasis, but the function of FGFs in mammary gland stem cells is less well defined. Both FGFR1 and FGFR2 are expressed in basal and luminal mammary epithelial cells (MECs), suggesting that together they might play a role in mammary gland development and stem cell dynamics. Previous studies have demonstrated that the deletion of FGFR2 resulted only in transient developmental defects in branching morphogenesis. Using a conditional deletion strategy, we investigated the consequences of FGFR1 deletion alone and then the simultaneous deletion of both FGFR1 and FGFR2 in the mammary epithelium. FGFR1 deletion using a keratin 14 promoter-driven Cre-recombinase resulted in an early, yet transient delay in development. However, no reduction in functional outgrowth potential was observed following limiting dilution transplantation analysis. In contrast, a significant reduction in outgrowth potential was observed upon the deletion of both FGFR1 and FGFR2 in MECs using adenovirus-Cre. Additionally, using a fluorescent reporter mouse model to monitor Cre-mediated recombination, we observed a competitive disadvantage following transplantation of both FGFR1/R2-null MECs, most prominently in the basal epithelial cells. This correlated with the complete loss of the mammary stem cell repopulating population in the FGFR1/R2-attenuated epithelium. FGFR1/R2-null MECs were partially rescued in chimeric outgrowths containing wild-type MECs, suggesting the potential importance of paracrine mechanisms involved in the maintenance of the basal epithelial stem cells. These studies document the requirement for functional FGFR signaling in mammary stem cells during development.
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更多信息

更多信息
种属 Mouse
克隆 M1/69
Gene Id 12484
Alternative Names CD24a, Heat stable antigen, HSA, Ly-52, Nectadrin, Small cell lung carcinoma cluster 4 antigen
同种型/isotype IgG2b, kappa
法律声明:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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