抗小鼠MHC II类（I-A/I-E）抗体，克隆M5/114.15.2，APC

抗小鼠MHC II类（I-A/I-E）的大鼠单克隆IgG2b抗体

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产品号 #100-0290_C

抗小鼠MHC II类（I-A/I-E）的大鼠单克隆IgG2b抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

M5/114.15.2（M5/114）抗体可特异性识别小鼠主要组织相容性复合物II类受体（MHC-II）上的胞外表位。MHC-II是一种异二聚跨膜糖蛋白，由一个α亚基与一个β亚基非共价结合而成。在小鼠中，MHC-II复合物被命名为I-A和I-E，分别包含Aα/Aβ和Eα/Eβ异源二聚体。MHC-II与CD3/T细胞受体（TCR）复合物以及CD4分子一起，介导抗原递呈细胞将外源抗原水解产生的肽递呈给CD4+ T（辅助性）细胞的关键功能，从而抑制或诱导对肽的免疫反应。M5/114.15.2抗体可识别I-Ab、I-Ad、I-Aq、I-Ed和I-Ek（但不包括I-Af、I-Ak或I-As） MHC II类同种抗原所共有的多态性决定因素，这些抗原在携带H-2b、d、p、q、r和u单倍型（但不包括H-2f或H-2s单倍型）的小鼠B细胞、树突状细胞、巨噬细胞、单核细胞和激活的T细胞上表达。研究发现，M5/114抗体抑制H-2b、d、q和u单倍型T细胞的I-A限制性反应（但不抑制H-2f、k或s单倍型），与NOD （H-2g7）小鼠细胞不发生反应。

亚型
一抗

靶抗原
MHC Class II (I-A/I-E)

别名
IA，I-A，IA/IE，I-A/E，I-E，MHC class II，MHC-II

活性物种
小鼠

偶联
APC，FITC，PE，未偶联的

宿主物种
大鼠

细胞类型
B 细胞，巨噬细胞，单核细胞，T 细胞

应用
CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，放射免疫分析，Western印迹

研究领域
免疫

克隆
M5/114.15.2

基因编号
14961/14969

同种型
IgG2b，kappa

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实验数据

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC or a rat IgG2b, kappa APC isotype control antibody.

Figure 1. Data for Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC (filled histogram) or a rat IgG2b, kappa APC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, FITC or a rat IgG2b, kappa FITC isotype control antibody.

Figure 2. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2, FITC

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, FITC (filled histogram) or a rat IgG2b, kappa FITC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, PE or a rat IgG2b, kappa PE isotype control antibody.

Figure 3. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2, PE

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, followed by a mouse anti-rat IgG2b antibody, PE or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, PE.

Figure 4. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2,Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, followed by a mouse anti-rat IgG2b antibody, PE (filled histogram) or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, PE (solid line histogram).