Anti-Mouse TER119 Antibody, Clone TER-119

总览

TER-119 单克隆抗体可识别小鼠 TER119（又称 Ly-76），这是一种分子量约为 52 kDa 的蛋白质，通常与糖蛋白 A（glycophorin A）相关联，存在于胚胎卵黄囊、胎儿和新生小鼠肝脏、成年骨髓、外周血及淋巴器官中的红系细胞表面。

TER119 是一种红系特异性标志物，在红系细胞从早期红系祖细胞（proerythroblasts）到成熟红细胞的整个分化阶段中均有表达，但不在红系集落形成单位（BFU-E，即爆裂形成单位红系细胞，或 CFU-E，即集落形成单位红系细胞）中表达。

TER-119 抗体是“lineage抗体混合物”中的组分，常用于检测或去除分化为造血系的细胞。在成年小鼠中，TER119 表达于约 20–25% 的骨髓细胞和约 2–3% 的脾细胞（splenocytes）中。

该抗体克隆已被验证可用于评估使用 EasySep™ 试剂盒分选获得的细胞的纯度，包括 EasySep™ 小鼠 CD4⁺ T 细胞分离试剂盒（产品号 #19852）。

分类
一抗

靶抗原
TER119

别名
Ly-76，TER-119

反应种属
小鼠

偶联
生物素，FITC，PE，PerCP-Cyanine5.5

宿主物种
大鼠

细胞类型
红系细胞，红细胞

种属
小鼠

应用
细胞分选，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
免疫

克隆
TER-119

Gene ID
104231

Isotype
IgG2b, κ轻链

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实验数据

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, followed by goat antimouse IgG, FITC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Image showing flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER-119 Antibody, Clone TER-119, Biotin followed by streptavidin (SAV) PE or a rat IgG2b, kappa isotype control antibody, Biotin followed by streptavidin (SAV) PE.

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER-119 Antibody, Clone TER-119, Biotin followed by streptavidin (SAV) PE (filled histogram) or a rat IgG2b, kappa isotype control antibody, Biotin followed by streptavidin (SAV) PE (solid line histogram).

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Figure 5. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).