The Nuts and Bolts of Lineage Chimerism Testing

Chimerism analysis is used to monitor the outcome of allogeneic hematopoietic stem cell transplantation. By looking at the presence of donor leukocytes within specific cell subsets of the host’s peripheral blood, one can assess the success of donor cell engraftment or detect early graft failure, rejection, or relapse. Prior to performing chimerism analysis, different cell subsets must be isolated from the same sample to perform lineage-specific analysis. In this webinar, Dr. Cathi Murphey (Immunogenetics Lab Director; Southwest Immunodiagnostics, Texas) will discuss available technologies for isolating different cell subsets from the same sample and improving cell purity prior to chimerism analysis.