搜索结果: 'easysep'

Long T et al. (MAR 2014) Biomaterials 35 9 2752--9

The effect of mesenchymal stem cell sheets on structural allograft healing of critical sized femoral defects in mice.

Structural bone allografts are widely used in the clinic to treat critical sized bone defects,despite lacking the osteoinductive characteristics of live autografts. To address this,we generated revitalized structural allografts wrapped with mesenchymal stem/progenitor cell (MSC) sheets,which were produced by expanding primary syngenic bone marrow derived cells on temperature-responsive plates,as a tissue-engineered periosteum. In vitro assays demonstrated maintenance of the MSC phenotype in the sheets,suggesting that short-term culturing of MSC sheets is not detrimental. To test their efficacy in vivo,allografts wrapped with MSC sheets were transplanted into 4-mm murine femoral defects and compared to allografts with direct seeding of MSCs and allografts without cells. Evaluations consisted of X-ray plain radiography,3D microCT,histology,and biomechanical testing at 4- and 6-weeks post-surgery. Our findings demonstrate that MSC sheets induce prolonged cartilage formation at the graft-host junction and enhanced bone callus formation,as well as graft-host osteointegration. Moreover,a large periosteal callus was observed spanning the allografts with MSC sheets,which partially mimics live autograft healing. Finally,biomechanical testing showed a significant increase in the structural and functional properties of MSC sheet grafted femurs. Taken together,MSC sheets exhibit enhanced osteogenicity during critical sized bone defect repair,demonstrating the feasibility of this tissue engineering solution for massive allograft healing. View Publication

产品类型：

产品号#：

19771

产品名：

EasySep™ 小鼠间充质干/祖细胞富集试剂盒
Hou TZ et al. ( 2015) The Journal of Immunology 194 5 2148--2159

A Transendocytosis Model of CTLA-4 Function Predicts Its Suppressive Behavior on Regulatory T Cells

Manipulation of the CD28/CTLA-4 pathway is at the heart of a number of immunomodulatory approaches used in both autoimmunity and cancer. Although it is clear that CTLA-4 is a critical regulator of T cell responses,the immunological contexts in which CTLA-4 controls immune responses are not well defined. In this study,we show that whereas CD80/CD86-dependent activation of resting human T cells caused extensive T cell proliferation and robust CTLA-4 expression,in this context CTLA-4 blocking Abs had no impact on the response. In contrast,in settings where CTLA-4(+) cells were present as regulators� View Publication

产品类型：

产品号#：

18062

18062RF

17951

17951RF

19155

19155RF

19232

19232RF

19052

19052RF

19059

19059RF

100-0695

产品名：

EasySep™人T细胞分选试剂盒

RoboSep™ 人T细胞分选试剂盒

EasySep™ 人CD4+CD127lowCD49d-调节性T细胞富集试剂盒

RoboSep™ CD4+ CD127low CD49d-调节性T细胞富集试剂盒

EasySep™人CD4+ T细胞富集试剂盒

RoboSep™ 人CD4+ T细胞富集试剂盒含滤芯吸头

EasySep™人单核细胞富集试剂盒

RoboSep™ 人单核细胞富集试剂盒含滤芯吸头

EasySep™人T细胞分选试剂盒
Gomez AM et al. (MAR 2015) The Journal of Immunology 194 5 2300--8

HIV-1-triggered release of type I IFN by plasmacytoid dendritic cells induces BAFF production in monocytes.

HIV-1 infection leads to numerous B cell abnormalities,including hypergammaglobulinemia,nonspecific B cell activation,nonspecific class switching,increased cell turnover,breakage of tolerance,increased immature/transitional B cells,B cell malignancies,as well as a loss of capacity to generate and maintain memory,all of which contribute to a global impairment of the immune humoral compartment. Several cytokines and soluble factors,which are increased in sera of HIV-1-infected individuals,have been suggested to directly or indirectly contribute to these B cell dysfunctions,and one of these is the B cell-activating factor (BAFF). We report in this study that HIV-1 (X4- and R5-tropic) upregulates BAFF expression and secretion by human monocytes. Moreover,we show that the virus-mediated production of BAFF by monocytes relies on a type I IFN response by a small percentage of plasmacytoid dendritic cells (pDCs) present in the monocyte cultures. HIV-1-induced type I IFN by pDCs triggers BAFF production in both classical and intermediate monocytes,but not in nonclassical monocytes,which nonetheless display a very strong basal BAFF production. We report also that basal BAFF secretion was higher in monocytes obtained from females compared with those from male donors. This study provides a novel mechanistic explanation for the increased BAFF levels observed during HIV-1 infection and highlights the importance of pDC/monocyte crosstalk to drive BAFF secretion. View Publication

产品类型：

产品号#：

19062

19062RF

19058

19058RF

100-1525

产品名：

EasySep™人浆细胞样DC富集试剂盒

RoboSep™ 人浆细胞样DC富集试剂盒含滤芯吸头

EasySep™人单核细胞富集试剂盒（不去除CD16）

RoboSep™ 人单核细胞富集试剂盒（不去除CD16）含滤芯吸头

EasySep™人单核细胞富集试剂盒（不去除CD16）
24:24

线上讲座
Streamline Your B Cell Research from Isolation to Expansion

发布日期: 07/24/2023
S. Balu et al. ( 2011) The Journal of Immunology 186 3113-3119

A novel human IgA monoclonal antibody protects against tuberculosis

Abs have been shown to be protective in passive immunotherapy of tuberculous infection using mouse experimental models. In this study,we report on the properties of a novel human IgA1,constructed using a single-chain variable fragment clone (2E9),selected from an Ab phage library. The purified Ab monomer revealed high binding affinities for the mycobacterial ?-crystallin Ag and for the human Fc?RI (CD89) IgA receptor. Intranasal inoculations with 2E9IgA1 and recombinant mouse IFN-? significantly inhibited pulmonary H37Rv infection in mice transgenic for human CD89 but not in CD89-negative littermate controls,suggesting that binding to CD89 was necessary for the IgA-imparted passive protection. 2E9IgA1 added to human whole-blood or monocyte cultures inhibited luciferase-tagged H37Rv infection although not for all tested blood donors. Inhibition by 2E9IgA1 was synergistic with human rIFN-? in cultures of purified human monocytes but not in whole-blood cultures. The demonstration of the mandatory role of Fc?RI (CD89) for human IgA-mediated protection is important for understanding of the mechanisms involved and also for translation of this approach toward development of passive immunotherapy of tuberculosis. View Publication

产品类型：

产品号#：

07801

19058

18060

18061

07861

07811

19058RF

100-1525

产品名：

EasySep™人单核细胞富集试剂盒（不去除CD16）

Lymphoprep™

Lymphoprep™

Lymphoprep™

Lymphoprep™

RoboSep™ 人单核细胞富集试剂盒（不去除CD16）含滤芯吸头

EasySep™人单核细胞富集试剂盒（不去除CD16）
科学海报
Isolation of Tumor-Infiltrating Leukocytes from Mouse Tumors

产品类型：

Conference：

AAI 2020

产品号#：

100-0350

产品名：

EasySep™小鼠TIL（CD45）正选试剂盒

发布日期: 10/22/2020
44:55:00

视频
Systemic Dysfunction and Plasticity of the Immune Macroenvironment in Cancer Models

发布日期: 07/15/2020
科学海报
Positive Selection of Hematopoietic Stem and Progenitor Cells from Adult Mouse Bone Marrow

产品类型：

Conference：

ASH 2003

产品号#：

产品名：
科学海报
Isolation of Highly Purified Mouse CD4+CD25+Foxp3+ Regulatory T Cells in Less

产品类型：

Conference：

AAI 2014

产品号#：

18782

18782RF

产品名：

EasySep™ 小鼠CD25调节性T细胞正选试剂盒

RoboSep™ 小鼠CD25调节性T细胞正选试剂盒
科学海报
Isolation of Eosinophils Without Ficoll or RBC Lysis

产品类型：

Conference：

AAI 2007

产品号#：

19256

19256RF

21000

20155

20119

产品名：

RoboSep™- S

RoboSep™分选管套装（9个塑料管）

RoboSep™ 吸头组件抛光剂
产品手册
Reliable Antibodies for Your Research

产品类型：

品牌：

EasySep

产品号#：

60100

60100.1

60100AD

60100AD.1

60102

60102FI

60104

60104AD

60104AD.1

60104AZ

60104AZ.1

60104BT

60104BT.1

60104BT.2

60104FI

60104FI.1

60104PE

60104PE.1

60106

60106.1

60106AZ

60106AZ.1

60106BT

60106BT.1

60106FI

60106FI.1

60106PE

60106PE.1

60107

60107.1

601

产品名：

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

抗β-微管蛋白III抗体，clone AA10，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3

抗小鼠TCR Gamma/Delta抗体，clone GL3，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3，APC

抗小鼠TCR Gamma/Delta抗体，clone GL3，APC

抗小鼠TCR Gamma/Delta抗体，clone GL3，Biotin

抗小鼠TCR Gamma/Delta抗体，clone GL3，Biotin

抗小鼠TCR Gamma/Delta抗体，clone GL3，FITC

抗小鼠TCR Gamma/Delta抗体，clone GL3，PE

抗小鼠TCR Gamma/Delta抗体，clone GL3，PE

抗人CD71（转铁蛋白受体）抗体，clone OKT9

抗人CD71（转铁蛋白受体）抗体，clone OKT9

抗人CD71（转铁蛋白受体）抗体，clone OKT9，APC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，APC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，Biotin

抗人CD71（转铁蛋白受体）抗体，clone OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，PE

抗人CD71（转铁蛋白受体）抗体，clone OKT9，PE
产品手册
使用RoboSep™ 进行全自动细胞分选

产品类型：

品牌：

EasySep

产品号#：

23000

23101

23102

23103

20104

20124

21000

20119

20155

19641

19641RF

19642

19642RF

19643

19643RF

19644

19644RF

20164

18644

18644RF

产品名：

RoboSep™-16

RoboSep™-16导电滤芯吸头, 无菌

RoboSep™-16导电滤芯吸头

RoboSep™-16垃圾袋, 每包100个

RoboSep™ 缓冲液

RoboSep™ 缓冲液（5X浓缩液）

RoboSep™- S

RoboSep™ 吸头组件抛光剂

RoboSep™分选管套装（9个塑料管）

EasySep™大鼠T细胞分选试剂盒

RoboSep™ 大鼠T细胞分选试剂盒

EasySep™大鼠CD4+ T细胞分选试剂盒

RoboSep™ 大鼠CD4+ T细胞分选试剂盒

EasySep™大鼠CD8+ T细胞分选试剂盒

RoboSep™ 大鼠CD8+ T细胞分选试剂盒

EasySep™大鼠B细胞分选试剂盒

RoboSep™ 大鼠B细胞分选试剂盒

RoboSep™ 缓冲液 2

EasySep™大鼠IgM去除试剂盒

RoboSep™ 大鼠IgM去除试剂盒