M. S. Fernandopulle et al. (JUN 2018)
Current protocols in cell biology 79 1 e51
Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons.
Accurate modeling of human neuronal cell biology has been a long-standing challenge. However,methods to differentiate human induced pluripotent stem cells (iPSCs) to neurons have recently provided experimentally tractable cell models. Numerous methods that use small molecules to direct iPSCs into neuronal lineages have arisen in recent years. Unfortunately,these methods entail numerous challenges,including poor efficiency,variable cell type heterogeneity,and lengthy,expensive differentiation procedures. We recently developed a new method to generate stable transgenic lines of human iPSCs with doxycycline-inducible transcription factors at safe-harbor loci. Using a simple two-step protocol,these lines can be inducibly differentiated into either cortical (i3 Neurons) or lower motor neurons (i3 LMN) in a rapid,efficient,and scalable manner (Wang et al.,2017). In this manuscript,we describe a set of protocols to assist investigators in the culture and genetic engineering of iPSC lines to enable transcription factor-mediated differentiation of iPSCs into i3 Neurons or i3 LMNs,and we present neuronal culture conditions for various experimental applications. {\textcopyright} 2018 by John Wiley & Sons,Inc.
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Andrade LNdS et al. (SEP 2012)
Human Molecular Genetics 21 17 3825--3834
Evidence for premature aging due to oxidative stress in iPSCs from Cockayne syndrome
Cockayne syndrome (CS) is a human premature aging disorder associated with neurological and developmental abnormalities,caused by mutations mainly in the CS group B gene (ERCC6). At the molecular level,CS is characterized by a deficiency in the transcription-couple DNA repair pathway. To understand the role of this molecular pathway in a pluripotent cell and the impact of CSB mutation during human cellular development,we generated induced pluripotent stem cells (iPSCs) from CSB skin fibroblasts (CSB-iPSC). Here,we showed that the lack of functional CSB does not represent a barrier to genetic reprogramming. However,iPSCs derived from CSB patient's fibroblasts exhibited elevated cell death rate and higher reactive oxygen species (ROS) production. Moreover,these cellular phenotypes were accompanied by an up-regulation of TXNIP and TP53 transcriptional expression. Our findings suggest that CSB modulates cell viability in pluripotent stem cells,regulating the expression of TP53 and TXNIP and ROS production.
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产品类型：
产品号#：
05850
05857
05870
05875
85850
85857
85870
85875
产品名：
mTeSR™1
mTeSR™1
Reference
Belle K et al. (JAN 2017)
Neuroscience letters 637 201--206
Generation of disease-specific autopsy-confirmed iPSCs lines from postmortem isolated Peripheral Blood Mononuclear Cells
Understanding the molecular mechanisms that underlie neurodegenerative disorders has been hampered by a lack of readily available model systems that replicate the complexity of the human disease. Recent advances in stem cell technology have facilitated the derivation of patient-specific stem cells from a variety of differentiated cell types. These induced pluripotent stem cells (iPSCs) are attractive disease models since they can be grown and differentiated to produce large numbers of disease-relevant cell types. However,most iPSC lines are derived in advance of,and without the benefit of,neuropathological confirmation of the donor - the gold standard for many disease classifications and measurement of disease severity. While others have reported the generation of autopsy-confirmed iPSC lines from patient explants,these methods require outgrowth of cadaver tissue,which require additional time and is often only successul 50% of the time. Here we report the rapid generation of autopsy-confirmed iPSC lines from peripheral blood mononuclear cells (PBMCs) drawn postmortem. Since this approach doesn't require the propagation of previously frozen cadaver tissue,iPSC can be rapidly and efficiently produced from patients with autopsy-confirmed pathology. These matched iPSC-derived patient-specific neurons and postmortem brain tissue will support studies of specific mechanisms that drive the pathogenesis of neurodegenerative diseases.
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产品类型：
产品号#：
05833
05850
05857
05870
05875
07801
07811
07851
07861
85450
85460
85850
85857
85870
85875
86450
86460
05835
05839
产品名：
STEMdiff™神经祖细胞培养基
Lymphoprep, 250mL
Lymphoprep™
Lymphoprep, 500mL
Lymphoprep™
SepMate™-50(试管)
SepMate™-50(试管)
mTeSR™1
mTeSR™1
SepMate™-50 (RUO)
SepMate™-50 (RUO)
STEMdiff™神经诱导培养基
STEMdiff™神经诱导培养基
Reference
E. Gabriel et al. (JAN 2016)
Stem cell reports 7 4 678--692
Development and Dynamic Regulation of Mitochondrial Network in Human Midbrain Dopaminergic Neurons Differentiated from iPSCs.
Mitochondria are critical to neurogenesis,but the mechanisms of mitochondria in neurogenesis have not been well explored. We fully characterized mitochondrial alterations and function in relation to the development of human induced pluripotent stem cell (hiPSC)-derived dopaminergic (DA) neurons. Following directed differentiation of hiPSCs to DA neurons,mitochondria in these neurons exhibit pronounced changes during differentiation,including mature neurophysiology characterization and functional synaptic network formation. Inhibition of mitochondrial respiratory chains via application of complex IV inhibitor KCN (potassium cyanide) or complex I inhibitor rotenone restricted neurogenesis of DA neurons. These results demonstrated the direct importance of mitochondrial development and bioenergetics in DA neuronal differentiation. Our study also provides a neurophysiologic model of mitochondrial involvement in neurogenesis,which will enhance our understanding of the role of mitochondrial dysfunctions in neurodegenerative diseases.
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EasySep™小鼠TIL（CD45）正选试剂盒
人iPSC衍生的前脑神经元前体细胞 人诱导多能干细胞系SCTi003-A分化的冻存的前脑神经元前体细胞
人iPSC衍生的神经祖细胞 冻存的人神经祖细胞由人诱导多能干细胞系SCTi003-A分化而来
健康对照人 iPSC 系，女性，SCTi003-A 人多能干细胞系，冷冻
健康对照人 iPSC 系，男性，SCTi004-A 人多能干细胞系，冷冻
Reference
30:53
WebinarHighly Characterized Human iPSCs and NPCs for Downstream Differentiation Applications发布日期: 7/19/23
iPSCdirect™ 一次性使用、免维持的人诱导多能干细胞，冷冻
