搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'
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Don't Want to Use Serum? Alternatives for Cell Separation Buffers When isolating cells using EasySep™ or RoboSep™ cell separation systems, the recommended buffer is 2% FBS in Phosphate Buffered Saline w/ 1 mM EDTA
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How to Prepare and Plate Semi-Solid Medium Steps involved with preparing and plating methylcellulose-based semi-solid media (e.g. MethoCult™ and ClonaCell™)
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Identification of Colonies Derived from Mouse Hematopoietic Progenitors Representative colony images and tips for identifying progenitor subtypes in CFU assays
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Harvesting cells from CFU assays Cells from colonies grown in methylcellulose-based medium can be harvested for further analysis or culture by plucking or washing them out of the medium
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How to Optimize Your T Cell Therapy Workflow—Without the Use of Serum or Feeder Cells In this talk, Dr. Dominika Nackiewicz introduces how STEMCELL Technologies can help optimize your T cell cultures with robust, high-performance products and expert support. You will learn how to generate high yields of functional T cells for cell therapy development and manufacturing using the new GMP ImmunoCult™ medium and activators. She will also discuss efficient gene editing strategies using the new CellPore™ Transfection System and solutions for T cell generation from pluripotent stem cells or hematopoietic stem and progenitor cells.
This webinar is part of the “Translational T Cell Talks: Scaling for the Future” event hosted in collaboration with Scientist.com on June 11, 2024. View the rest of the talks and read the Q&A transcripts. -
Advances in Point-of-Care Cell Engineering—What Are You Feeding Your CAR T Cells? In this webinar, Dr. Julian J. Lum discusses key factors to consider when choosing metabolic assays and how they can be used to assess cell function. Additionally, he discusses the metabolic characteristics of different commercially available cell culture media formulations as well as the importance of assessing the array of media products and their impact on metabolism.
This webinar is part of the “Translational T Cell Talks: Scaling for the Future” event hosted in collaboration with Scientist.com on June 11, 2024. View the rest of the talks and read the Q&A transcripts. -
Serum-Free Expansion of CD34+ Cells with SFEM II and UM729 Explore the use of StemSpan™ SFEM II medium, StemSpan™ CD34+ Expansion Supplement and small molecules UM171 or UM729 to enhance expansion of HSPCs