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Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types CRISPR-Cas genome editing in cell culture systems is a powerful technique for disease modeling and the development of cellular therapies. Compared to work with immortalized cell lines, genome editing of stem and primary cells presents unique challenges, including issues related to efficient delivery and expression of CRISPR machinery, clonogenicity, and cytotoxicity. In this webinar, our in-house expert, Dr. Ashley Watson, discusses optimized workflows for CRISPR-Cas9 genome editing in human pluripotent stem cells and primary T cells.

ClonaCell™-HY Procedure Selection and growth of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY including preparation of myeloma cells and splenocytes, fusion, selection and cloning, and harvesting

Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells

Mammalian Cell Cloning Procedure: Customizable Semi-Solid Cloning with ClonaCell™ FLEX Medium This video outlines the ClonaCell™ FLEX procedure for semi-solid cloning, including preparation of the cells, plating, colony picking, and expansion

David Knapp, PhD Dr. David Knapp shares his work examining how growth factors affect the survival, proliferation, and maintenance of cells of the cord blood HSC compartment

Isolating Cells from Atypical Samples Technical tip from our dedicated team of Product and Scientific Support specialists

Isolation of Neutrophils from Mouse Bone Marrow and Blood Using a Rapid .

Enhance Sensitivity of Multiple Myeloma Testing with Purified CD138+ Plasma Cells .

Procedure for Negative Enrichment of Monocytes from Mouse Blood and Bone Marrow .

Method for Negative Enrichment of Monocytes from Mouse Blood and Bone Marrow .

How to Generate Neural Progenitor Cells from hPSCs Using STEMdiff™ Neural Induction Medium STEMdiff™ Neural Induction Medium is a defined, serum-free and highly efficient medium for the differentiation of hESCs & iPSCs to NPCs

STEMdiff™ Neural Induction Medium .