ClonaCell™-CHO AOF 添加物

总览

通过在基础培养基中使用ClonaCell™-CHO AOF添加物，提高克隆效率，促进中国仓鼠卵巢（CHO）细胞的强劲生长，从而节省时间和保护宝贵的克隆细胞。配方不含酚红，与ClonaCell™-CHO ACF 添加物（产品号：#03820）相比，该添加物提供无沉淀培养，可提高成像清晰度和克隆性可视化。

这款无动物来源（AOF）添加物在二级生产过程中不含动物来源的原材料。它含有重组蛋白和化学成分明确，不含血清、水解物、L -谷氨酰胺或选择剂。此40X添加物可与多种适用于CHO 细胞培养的基础培养基一起使用，包括无蛋白ClonaCell™-CHO CD液体培养基（产品号：#03817）或半固体ClonaCell™-CHO CD培养基（产品号：#03815）。在ClonaCell™品牌页面中探索高效细胞克隆和细胞系生成的液体和半固体培养基。了解更多关于我们如何定义我们的培养基和添加物，包括AOF培养基。

亚型
添加剂

细胞类型
CHO细胞

种属
其它细胞系

应用
细胞培养

品牌
ClonaCell

研究领域
抗体制备，细胞系制备，药物发现和毒理检测

制剂类别
Animal Origin-Free，无血清

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实验数据

Comparison of the cloning efficiency (%) of CHO-S and CHO-K1 cells subcloned in DMEM + 10% FBS, or ClonaCell™-CHO CD Liquid Medium containing either ClonaCell™-CHO ACF Supplement or ClonaCell™-CHO AOF Supplement.

Figure 1. Cloning Efficiencies for Subcloned CHO-S and CHO-K1 Cells

CHO-S (A) and CHO-K1 (B) cells were subcloned by limiting dilution in DMEM + 10% FBS, or ClonaCell™-CHO CD Liquid Medium containing either ClonaCell™-CHO ACF Supplement or ClonaCell™-CHO AOF Supplement; all conditions contained 6 - 8 mM L-glutamine. Individual wells of 96-well plates were seeded with an average of 0.5 or 1 cell per well in 200 μL culture medium. After incubation for 14 days (37°C, 5% CO2) the plates were examined under a microscope and assessed for growth, with wells containing >100 cells considered positive for outgrowth. The cloning efficiency was estimated by Poisson statistics using the ELDA method described by Hu & Smith (2009). Data are shown as mean ± 1 SD for n = 4 independent experiments.