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ClonaCell™-HY AOF膨胀培养基

含有次黄嘌呤和胸腺嘧啶的杂交瘤扩增培养基（无动物源性）
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产品号 #03835_C

含有次黄嘌呤和胸腺嘧啶的杂交瘤扩增培养基（无动物源性）

产品优势

  • 动物origin-free
  • 血清的缺失减少了培养基中表现的可变性
  • 简化下游克隆筛选和抗体纯化（无血清源性IgG）
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

ClonaCell™-HY AOF扩增培养基是一种无血清、无动物源成分的液体培养基，专为杂交瘤在次黄嘌呤-氨基蝶呤-胸苷（HAT）筛选后的扩增而优化。该培养基含有次黄嘌呤和胸苷（HT），用于使杂交瘤脱离筛选过程中使用的氨基蝶呤。ClonaCell™-HY AOF扩增培养基能促进杂交瘤高效扩增，实现从含血清到无血清培养基的顺利过渡，并能使杂交瘤细胞系的活性和抗体产量稳定。大多数杂交瘤细胞可直接从含血清培养基切换至ClonaCell™-HY AOF扩增培养基，无需适应步骤。无血清的液体培养基有助于检测和纯化来自目的杂交瘤的抗体，避免血清来源免疫球蛋白的干扰。

该培养基已验证适用于小鼠和大鼠杂交瘤，并支持小鼠骨髓瘤（如Sp2/0）、大鼠骨髓瘤（如YB2/0）及人骨髓瘤（如U266）的扩增。在大多数情况下，冻存的杂交瘤可直接解冻至ClonaCell™-HY AOF扩增培养基，同时保持高存活率。本培养基可作为ClonaCell™-HY Medium A（产品号 #03801）或ClonaCell™-HY Medium E（产品号 #03805）的无血清替代方案。该培养基及其成分的生产中，至少在次级原材料水平不含任何动物源或人源成分。

包含
• 次黄嘌呤和胸苷 (HT) • 庆大霉素 • 酚红 • L-谷氨酰胺及其他添加物 • 其他成分，包括重组蛋白
 
分类
专用培养基
 
细胞类型
杂交瘤细胞
 
种属
人，小鼠，大鼠
 
应用
细胞培养
 
品牌
ClonaCell
 
研究领域
抗体​制备，细胞系制备，杂交瘤制备
 
制剂类别
无血清
 

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实验数据

Cloning Efficiencies for Three Hybridoma Cell Lines Subcloned in Serum-containing and Serun-free Cell Culture Media

Figure 1. Cloning Efficiencies for Three Hybridoma Cell Lines Subcloned in Serum-containing and Serum-free Cell Culture Media

The hybridoma lines were adapted to growth in each medium and subcloned by limiting dilution (n = 1 - 5). After incubation for 10 days (37°C, 5% CO2), the plates were analyzed with a Cell Metric™ instrument (Solentim). The plating efficiency was estimated by Poisson statistics using the ELDA method described by Hu & Smith, 2009 (J Immunol Meth, 347: 70-78). ACF = animal component-free. Data is expressed as mean + 1 SD.

Graph comparing growth of hybridoma cell line expanded in commercially available serum-containing and serum-free hybridoma cell culture media.

Figure 2. Expansion of an Established Hybridoma Cell Line in Several Commercially-Available Serum-Containing and Serum-Free Hybridoma Cell Culture Media

The hybridoma line was adapted to growth in 13 different cell culture media: ClonaCellTM-HY AOF Expansion Medium (animal origin-free), DMEM + 10% FBS or ClonaCellTM-HY Medium E (serum-containing), Medium 1 (serum-free with serum-derived proteins), Medium 2 – 4 (serum-free), Medium 5 (animal component-free), Medium 7, 9 (protein-free), and Medium 11 – 13 (chemically-defined). They were then seeded in triplicate in 24-well tissue culture plates at 5 x 103 cells/mL. The cultures were incubated at 37°C (5% CO2 atmosphere) and the viable cell density was measured on days 3 – 7. Data is expressed as mean ± 1SD.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ClonaCell™HY AOF Expansion Medium
Catalog #
03835
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
ClonaCell™-HY AOF Expansion Medium
Catalog #
03835
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ClonaCell™HY AOF Expansion Medium
Catalog #
03835
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
种属 Human, Mouse, Rat
Contains • Hypoxanthine and thymidine (HT) • Gentamicin • Phenol red • L-Glutamine and other supplements • Other ingredients, including recombinant proteins
配方类别 Serum-Free
质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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